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transgenic line

Et(fos:Gal4-VP16)s1026t

Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.


External Links:

 ZFIN

Lab or Origin:


Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.


Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Tg(atoh7 :gapRFP)cu2Tg

Tg(atoh7 :gapRFP)cu2Tg

About

 atonal bHLH transcription factor 7 is turned on in retinal ganglion cells as they transition from proliferating neuroblast to differentiated neuron. The atoh7 promoter in this transgenic line drives the expression of a membrane tagged version of RFP so the axons of the RGCs are also labelled so the optic nerve and tract projecting from the retina to the optic tectum can be distinguished.


External Links:

 ZFIN

Lab or Origin: Bill Harris Lab


Expressed in: 

Retinal ganglion cells, cilliary marginal zone, retina, optic nerve. optic tract, optic tectum.  


Key Publications

 Zolessi, F.R., Poggi, L., Wilkinson, C.J., Chien, C.B., and Harris, W.A. (2006)
Polarization and orientation of retinal ganglion cells in vivo.
Neural Development. 1:2.

Tg(slc17a6b: DsRed)nns9Tg

Tg(slc17a6b: DsRed)nns9Tg

About

 Slc17a6b is a vesicular glutamate transporter that that mediates the uptake of the excitatory neurotransmitter glutamate into vesicles in the presynaptic terminals of excitatory neurons. This BAC transgenic line from the Yoshihara lab drives the expression of DSRed in glutamatergic neurons. Expression can be seen in many neurons throughout the brain. Strong expression in the dorsal and ventral habenular subnuclei, olfactory bulbs, the pallium and optic tectum


External Links:

 ZFIN

Lab or Origin: Yoshihara Lab


Expressed in: 

 glutamatergic neurons, olfactory epithelium, olfactory bulb, pallium, subpallium, dorsal habenula, ventral habenula, preoptic area, prethalamus, pretectum (AF9), optic tectum, trigeminal sensory ganglion.


Key Publications

 
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009)
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish.
The Journal of neuroscience. 29(15):4756-4767.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum.
Developmental Biology. 343(1-2):1-17.

gSA2AZGFF 398A

gSA2AZGFF 398A

About

 a Gal4 enhancer-trap line form the lab of Koichi Kawakami that labels olfactory sensory neurons, hypothalamus and pituitary.


External Links:

 ZFIN

Lab or Origin: Kawakami Lab


Expressed in: 

olfactory sensory neurons, olfactory bulb glomeruli, hypothalamus and pituitary.  


Key Publications

 Asakawa, K., and Kawakami, K. (2009)
The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.
Methods (San Diego, Calif.). 49(3):275-281.

gSAIZGFFD812A

gSAIZGFFD812A

About

 GFP enhancer-trap line from the Kawakami lab that labels the olfactory bulb, pallium, habenula, optic tectum and cerebellum and hindbrain.


External Links:

 ZFIN

Lab or Origin: Kawakami Lab


Expressed in: 

 olfactory bulb, pallium, habenula, optic tectum and cerebellum and hindbrain.


Key Publications

 Asakawa, K., and Kawakami, K. (2009)
The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.
Methods (San Diego, Calif.). 49(3):275-281.

SAGFF(LF)223A

SAGFF(LF)223A

About

 A GFP enhancer trap line from the Kawakami lab that labels the olfactory bulb, pallium, pineal, habenula, pretectum, optic tectum, torus longitudinalis, hypothalamus, pituitary.


External Links:

 ZFIN

Lab or Origin: Kawakami Lab


Expressed in: 

  olfactory bulb, pallium, pineal, habenula, pretectum, optic tectum, torus longitudinalis, hypothalamus, pituitary.


Key Publications

 Asakawa, K., and Kawakami, K. (2009)
The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.
Methods (San Diego, Calif.). 49(3):275-281.

Tg(glyt2:GFP)/Tg(slc6a5:GFP)

Tg(glyt2:GFP)/Tg(slc6a5:GFP)

About

 Synonyms: Tg(slc6a5:GFP)

Previously called glycine transporter 2 now named solute carrier family 6 member 5(slc6a5), this trangemic line labels glycinergic neruons.


External Links:

 ZFIN

Lab or Origin: Joe Fetcho Lab


Expressed in: 

 glycinergic neurons, spinal cord, pineal, hindbrain.


Key Publications

 

Tg(isl1:GFP)rw0

Tg(isl1:GFP)rw0

About

 This transgenic line expresses GFP in cranial motor neurons. It was made using a construct that fuses Islet-1 promotor/enhancer sequences to GFP.

isl1 cranial nerves expanded-01.png

isl1 cranial nerves expanded-02.png

More images of this transgenic

External Links:

 ZFIN

Lab or Origin: Okomoto Lab


Expressed in: 

cranial motor neurons, hindbrain, subpallium, preoptic area. 


Key Publications

Higashijima, S., Hotta, Y., and Okamoto, H. (2000)
Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer.
The Journal of neuroscience. 20(1):206-218

Suli, A., Mortimer, N., Shepherd, I., and Chien, C.B. (2006)
Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons.
The Journal of neuroscience. 26(51):13328-13337.

Schoppik, D., Bianco, I.H., Prober, D.A., Douglass, A.D., Robson, D.N., Li, J.M.B., Greenwood, J.S.F., Soucy, E., Engert, F., Schier, A.F. (2017)
Gaze-stabilizing central vestibular neurons project asymmetrically to extraocular motoneuron pools. The Journal of neuroscience. 37(47):11353-11365.

Rebman, J.K., Kirchoff, K.E., Walsh, G.S. (2016)
Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons.
PLoS One. 11:e0164433.

Barsh, G.R., Isabella, A.J., Moens, C.B. (2017)
Vagus Motor Neuron Topographic Map Determined by Parallel Mechanisms of hox5 Expression and Time of Axon Initiation.
Current biology : CB. 27(24):3812-3825.e3.

Cox, J.A., Lamora, A., Johnson, S.L., and Voigt, M.M. (2011)
Diverse mechanisms for assembly of branchiomeric nerves.
Developmental Biology. 357(2):305-17.

Tg(oxt:EGFP)

Tg(oxt:EGFP)

About

 This transgenic construct drives GFP expression in oxytocin expressing neurons in the neuro-secretary pre-optic area(NPO) part of the neuroendocrine system.


External Links:

 ZFIN

Lab or Origin: Gil Levkowitz Lab


Expressed in: 

preoptic area, neurosecretory preoptic area (NPO).  


Key Publications

Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G. (2011)
The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary.
Developmental Cell. 21(4):642-654.


Machluf, Y., Gutnick, A., and Levkowitz, G. (2011)
Development of the zebrafish hypothalamus.
Annals of the New York Academy of Sciences. 1220(1):93-105.

Et(krt4:EGFP)sqet11

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.


External Links:

 ZFIN

Lab or Origin: Korzh Lab


Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary


Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.


External Links:

 ZFIN

Lab or Origin: Skarmeta Lab


Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum


Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Et(krt4:EGFP)sqet33

Et(krt4:EGFP)sqet33

About

 Enhancer trap line from Vladimir Korzh lab that has EGFP expression in the roof plate.


External Links:

 ZFIN

Lab or Origin: Korzh Lab


Expressed in: 

 roof plate


Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Kondrychyn, I., Teh, C., Sin, M., and Korzh, V. (2013) Stretching morphogenesis of the roof plate and formation of the central canal. PLoS One. 8(2):e56219.

sox3h10:EGFP

sox3h10:EGFP

About

 


External Links:

 ZFIN

Lab or Origin: Tom Becker Lab


Expressed in: 

 ciliary marginal zone of retina,pallium, pineal, habenula, hindbrain


Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

About

 


The construct used to create this enhancer trap line contains the highly conserved noncoding element  "hs7" from the regulatory region of the human SOX3 gene.


External Links:

 ZFIN

Lab or Origin: Tom Becker Lab


Expressed in: 

 olfactory bulb, subpallium, habenula, AF9 pretectum, optic tectum, cerebellum.


Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.


 Tg(3Hsa.SOX3-gata2a:EGFP)bi81Tg

Tg(3Hsa.SOX3-gata2a:EGFP)bi81Tg

About

 This construct contains the highly conserved noncoding element  "hs5" from the  regulatory region of the human SOX3 gene.



External Links:

 ZFIN

Lab or Origin: Tom Becker Lab


Expressed in: 

pineal, parapineal, pretectum, thalamus

 


Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.

Tg(-2.7shh:GFP)

Tg(-2.7shh:GFP)

About

The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish  two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.


All images of shh:GFP were produced by Monica Folgueira.

External Links:

 ZFIN

Lab or Origin: Nüsslein-Volhard, Christiane


Expressed in: 

basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.

 


Key Publications

 Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.

Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176

Et(fos:Gal4-VP16)s1168t

Et(fos:Gal4-VP16)s1168t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. Driving Kaede expression in the posterior tuberculum,hypothalamus and cerebellum. There is a very interesting tract labelled that projects between the midbrain tegmentum and posterior tuberculum. The tract skirts the tectal neuropil. The insertion of this transgene is currently unmapped.


External Links:

 ZFIN

Lab or Origin: Baier Lab


Expressed in: 

tectum, posterior tuberculum, tegmentum, hypothalamus, cerebellum. 


Key Publications

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Heap, L.A., Goh, C.C., Kassahn, K.S., and Scott, E.K. (2013) Cerebellar output in zebrafish: an analysis of spatial patterns and topography in eurydendroid cell projections. Frontiers in neural circuits. 7:53.

Heap, L.A., Vanwalleghem, G.C., Thompson, A.W., Favre-Bulle, I., Rubinsztein-Dunlop, H., Scott, E.K. (2018) Hypothalamic Projections to the Optic Tectum in Larval Zebrafish. Frontiers in Neuroanatomy. 11:135.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum. Developmental Biology. 343(1-2):1-17. 

Et(fos:Gal4-VP16)s1137t

Et(fos:Gal4-VP16)s1137t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It shows dense expression of kaede in the telencephalon and throughout all the habenula subnuclei. The insertion of this transgene is currently unmapped.


External Links:

 ZFIN

Lab or Origin: Baier Laboratory


Expressed in: 

 telencephalon, posterior tuberculum, habenula, hypothalamus


Key Publications

Mason, L., Scott, E.K., Staub, W., Finger-Baier, K., and Baier, H. (2009) Expression patterns from GAL4 enhancer trap screen. ZFIN Direct Data Submission. . (http://zfin.org).

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13. 

Tg(1.4dlx5a-dlx6a:GFP)ot1

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

 


Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 


More images

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab


Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

 


Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Tg(slc6a3:EGFP)ot80

Tg(slc6a3:EGFP)ot80

About

The Tg(slc6a3:EGFP)ot80 transgenic line expresses GFP under the control of
cis-regulatory elements of the dopamine transporter (DAT) gene slc6a3.

 


External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab



Transgene expressed in: 

olfactory bulb, subpallium, preoptic region, pretectum, posterior tuberculum, hypothalamus, optic tectum, cerebellum

Key Publications

 

Xi Y1, Yu M, Godoy R, Hatch G, Poitras L, Ekker M.
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19.