ZF Green Lab have just published on protocols.io our “Green” WHOLE-MOUNT IN SITU HYBRIDIZATION/ Immunostaining/TUNEL on zebrafish embryos protocol.
https://dx.doi.org/10.17504/protocols.io.81wgboxp3lpk/v1
We want to encourage other scientists to reduce and reuse lab plastic as much as possible in their experiments. Here we present our standard in-situ/immuno/TUNEL protocols with specific advice on how we minimise our use of plastic tips, pipettes and tubes-with no negative repercussions for our experiments.
We include lots of images for how we organise and store our various lab plastics so we can use them again and again. You will be amazed at how few fresh tips you need for your experiments. If everyone followed this guidance the reduction in plastic waste generated in the life sciences would be huge!
Please note the reason we made this protocol is to encourage you to use less plastic so it is by no means the shortest version of these protocols. Once you are set up with your reusable pipettes and tips you can switch to using the normal protocols.
For more tips on increasing the sustainability of your laboratory visit our website https://zebrafishucl.org/#zebrafish-green-lab
and check out our other green protocols on protocol.io
Reusing tips for gel loading
https://dx.doi.org/10.17504/protocols.io.e6nvw495dlmk/v1
Washing petri dishes for reuse in the lab and Fish Facility
