Tg(lhx5:GFP)b1205

About

 This transgenic line recapitulates the expression of lhx5 transcription factor. Generated by the Westerfield lab using BAC transgenesis. A kaede version of this line is also available. This transgenic has been used to study pathfinding in early telencephalic development (Guo et al., 2012; Zhang et al., 2012, Turner et al., 2019) and also to look at areas afferent to the habenula at later stages of development (Turner et al., 2016).

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Tg(lhx5:GFP) 4dpf lateral view

GFP+ populations in the forebrain innervate all habeunula subnuclei.

Abbreviations: Dp; posterior area of the pallium; Hb, habenula; OB, olfactory bulb; PO, preoptic area; PTh, prethalamus; tHC, tract of the habenula commissure; StMed, stria medularis; vENT, ventral entopeduncular nucleus.

Figure taken from Turner et al., 2016.

Tg(lhx5:GFP) lateral view 48hpf.

GFP+ populations in the forebrain innervate all habeunula subnuclei.

Abbreviations: Dp; posterior area of the pallium; Hb, habenula; OB, olfactory bulb; PO, preoptic area; PTh, prethalamus; tHC, tract of the habenula commissure; StMed, stria medularis; vENT, ventral entopeduncular nucleus.

Figure taken from Turner et al., 2016.

Dorsal view of the habenula in a 4dpf Tg(lhx5:GFP) larva.

All habenula subnuclei are innervated by GFP+ processes in the Tg(lhx5:GFP) line.

Abbreviations: dHbl, lateral subnucleus of the dorsal habeula; dHbm, medial subnucleus of the dorsal habenula; vHb, ventral subnucleus of the habenula.

lateral view of an adult Tg(lhx5:GFP) zebrafish

the ventral entopeduncular nucleus(vENT) is strongly labelled in this transgenic line.

Abbreviations: ac, anterior commissure; Pal, pallium; OB, olfactory bulb; vENT, ventral entopeduncular nucleus.

Image from Turner et al., 2016.

transverse section through the telencephalon of a Tg(lhx5:GFP) zebrafish.

the ventral entopeduncular nucleus(vENT) is strongly labelled in this transgenic line.

Abbreviations: ac, anterior commissure; dENT, dorsal entopeduncular nucleus; Dp, posterior area of the pallium; vENT, ventral entopeduncular nucleus.

Image from Turner et al., 2016.

transverse section through the habenula of an adult Tg(lhx5:GFP) zebrafish

All habenula subnuclei are innervated by GFP+ processes in the Tg(lhx5:GFP) line.

Image from Turner et al., 2016

Tg(lhx5:GFP)(cyan) 4dpf dorsal view with SV2(magenta)

Tg(lhx5:GFP)(cyan) 4dpf ventral view with SV2(magenta)

Tg(lhx5:GFP)(cyan) 4dpf lateral view

Tg(lhx5:GFP)(cyan) 4dpf lateral view with SV2(magenta)

External Links:

 ZFIN

Lab or Origin: Westerfield Lab

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Expressed in: 

 olfactory bulb, pallium, ventral entopeduncular nucleus, preoptic area, prethalamus, stria medularis, tract of the habenula commissure, habenula commisure, habenula neuropil.

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Key Publications

Gao, J., Zhang, C., Yang, B., Sun, L., Zhang, C., Westerfield, M., and Peng, G. (2012)
Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish.
PLoS One. 7(5):e36516.

Zhang, C., Gao, J., Zhang, H., Sun, L., and Peng, G. (2012)
Robo2-Slit and Dcc-Netrin1 Coordinate Neuron Axonal Pathfinding within the Embryonic Axon Tracts.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(36):12589-12602.

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30.

Turner, K.J., Hoyle, J., Valdivia, L.E., Cerveny, K.L., Hart, W., Mangoli, M., Geisler, R., Rees, M., Houart, C., Poole, R.J., Wilson, S.W., Gestri, G. (2019)
Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits.
PLoS One. 14:e0211073.