Tg(-2.7shh:GFP)

About

The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish  two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.

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All images of shh:GFP were produced by Monica Folgueira.

4dpf lateral shh:GFP with acetylated tubulin(cyan) and sytox(grey)

pHb, habenula; lfb, lateral forebrain bundle; OB, olfactory bulb; OT, optic tectum; ot, optic tract; Pal, pallium; PT, posterior tuberculum; PTh, prethalamus; Th, thalamus; ZLI, zona-limitans intrathalamica.

shh:GFP, tubulin and sytox 4dpf lateral forebrain

shh:GFP and tubulin. 4 dpf lateral forebrain

shh:GFP and tubulin. 4dpf dorsal view

single z-slice through telencephalon

shh:GFP, tublin and sytox at 4 dpf showing GFP + processes innervating the telencephalon.

shh:GFP dorsal view of 4dpf larvae

Frontal transverse z-slice through the telencephalon of shh:GFP

shh:GFP, tublin and sytox at 4.5dpf showing GFP + processes innervating the telencephalon.

shh:GFP and tubulin, 4dpf lateral view

shh:GFP lateral view 4dpf

shh:GFP, tubulin 4dpf ventral view

Shows expression in floor plate, posterior tuberculum and hypothalamus

shh:GFP and tubulin 24hpf lateral view

shh:GFP lateral forebrain at 24hpf

Shows expression in floor plate and zona limitans intrathalamica.

shh:GFP and tubulin lateral view of 24hpf

External Links:

 ZFIN

Lab or Origin: Nüsslein-Volhard, Christiane

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Expressed in: 

basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.

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Key Publications

 Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.

Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176