About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.

Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area.

More images

Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green), SV2(cyan) and tubulin(red) antibodies.

by Monica Folgueira

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP and anti-tubulin(cyan).

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP.

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green).

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and anti-tubulin antibodies.

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and tubulin(cyan) antibodies.

by Kate Turner

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

by KateTurner

Olfactory glomeruli in Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

Frontal view showing the GFP +processes of olfactory bulb interneurons innervating the glomeruli.

Monica Folgueira

24hpf dorsal view of dlx:GFP with tubulin

Tubulin shows the longitudinal axon scaffold formed by this early stage. Several clusters of dlx:GFP + cells are present in the telencephalon, diencephalon, midbrain and hindbrain.

Monica Folgueira

Olfactory bulb Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

single section through a 4dpf olfactory bulb.

Monica Folgueira

3dpf frontal view of dlx:GFP larvae

Monica Folgueira

3dpf dorsal view dlx:GFP

Showing many GFP+ neurons in the optic tectum and cerebellum.

Monica Folgueira

2dpf dlx:GFP ventral view

Shows cells throughout the subpallium and preoptic area and hypothalamic lobes.

Monica Folgueira

3dpf lateral view of dlx:GFP in forebrain

Monica Folgueira

3dpf late view of dlx:GFP hindbrain

showing dense cluster of GFP+ cells in cerebellum and scattered cells in the spinal cord.

Monica Folgueira

3dpf dorsal view of dlx:GFP hindbrain

Monica Folgueira

3dpf frontal section of dlx:GFP subpallium

Monica Folgueira

3dpf thick section of dlx:GFP through midbrain/diencephalon

Shows tectal and pretectal expression.

Monica Folgueira

External Links:

ZFIN

Lab of Origin: Mark Ekker Lab

Expressed in:

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000)
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011)
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development.
Int. J. Dev. Neurosci.. 29(7):681-91.