About gad1a (glutamate decarboxylase 1a)
gad1a is orthologous to human GAD1 /GAD67(glutamate decarboxylase 1) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad1a is expressed in the nervous system in neurons that use GABA as a neurotransmitter.
Expression pattern
gad1a is expressed in GABAergic neurons throughout the nervous system.
Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probes against gad1a. This probe labels GABA-ergic neurons.
Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against gad1a.
6dpf brain of a larval zebrafish labeled with a probe against gad1a FISH. this stack was morphed to ZBB. This screenshot shows the gad1a stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this gad1a data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.
Selected images
All imaging by Chintan Trivedi.
External links
Addgene probe link
Download the gad1a .png file to view the data using Zebrafish Brain Browser
FISH Protocol
Key Publications
Martin, S.C., Heinrich, G., and Sandell, J.H. (1998)
Sequence and expression of glutamic acid decarboxylase isoforms in the developing zebrafish.
The Journal of comparative neurology. 396:253-266.
Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.