About vglut2
Synonyms: slc17A6 (solute carrier family 17 member 6), VGLUT2.2. vglut2a, vglut2b.
vglut2 encodes a vesicular glutamate transporter. Due to genome duplication in zebrafish two copies of the vglut2 or slc17A6 exist in zebrafish. The probe used for this FISH is a cocktail that contains probes specific to both the vglut2a and vglut2b genes.
Expression pattern
vglut2 is expressed in excitatory glutamatergic neurons. The expression pattern of this gene is very widespread, neurons in all brain regions express vglut2.
Selected images
All FISH and imaging done by Chintan Trivedi using a lightsheet microscope.
Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a cocktail of probes against vglut2. This probe labels glutamatergic neurons.
Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a cocktail of probes against vglut2.
6dpf brain of a larval zebrafish labeled with a cocktail probe against vglut2 FISH. this stack was morphed to ZBB. This screenshot shows the vglut2 stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this vglut data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.
External links
Addgene probe link
Download the vglut2 .png file to view the data using Zebrafish Brain Browser
FISH Protocol
Publications
Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.