About tac3a
tachykinin precursor 3a
synonyms: Neurokinin B
tac3a is orthologous to human TAC3 and in rodents to tac2. TAC3/Neurokinin B may participate in neuroendocrine control of zebrafish reproduction by stimulating gonadotropin-releasing hormone (GnRH) neurons, mainly via kisspeptin. In mammals, NeurokininB is coexpressed with kisspeptin (Kiss) and dynorphin in neurons of the arcuate nucleus of the hypothalamus, which are therefore called KNDy neurons
Expression pattern
At embryonic and larval stages tac3a is expressed asymmetrically in the dorsal habenula. tac3a mRNA-expressing cells detected in the subpallium (SubP), posterior tuberculum(PT) , rostral and intermediate hypothalamus, posterior tuberal nucleus (PTN), tegmentum(Teg), superior raphe (SR) and the medulla oblongata.
Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against tac3a.
Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against tac3a.
Z projection of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against tac3a.
6dpf brain of a larval zebrafish labeled with a probe against tac3a . this stack was morphed to ZBB. This screenshot shows the tac3a stack and a HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.
Selected images
All imaging by Chintan Trivedi on a lightsheet microscope.
External links
Addgene probe link
Download the tac3a .png file to view the data using Zebrafish Brain Browser
FISH Protocol
Key Publications
Zhou, W., Li, S., Liu, Y., Qi, X., Chen, H., Cheng, C.H., Liu, X., Zhang, Y., and Lin, H. (2012)
The evolution of tachykinin/tachykinin receptor (TAC/TACR) in vertebrates and molecular identification of the TAC3/TACR3 system in zebrafish (Danio rerio).
Molecular and Cellular Endocrinology. 361(1-2):202-212.
deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E. (2014) Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish.
Genesis (New York, N.Y. : 2000). 52(6):636-55.
Pandey, S., Shekhar, K., Regev, A., Schier, A.F. (2018)
Comprehensive Identification and Spatial Mapping of Habenular Neuronal Types Using Single-Cell RNA-Seq. Current biology : CB. 28(7):1052-1065.e7.
Ogawa, S., Ramadasan, P.N., Goschorska, M., Anantharajah, A., Ng, K.W., and Parhar, I.S. (2012)
Cloning and expression of tachykinins and their association with kisspeptins in the brains of zebrafish.
The Journal of comparative neurology. 520(13):2991-3012.
Biran, J., Palevitch, O., Ben-Dor, S., and Levavi-Sivan, B. (2012)
Neurokinin Bs and neurokinin B receptors in zebrafish-potential role in controlling fish reproduction. Proceedings of the National Academy of Sciences of the United States of America. 109(26):10269-10274.