Viewing entries in
transgenic line

NPY (neuropeptide Y)

NPY (neuropeptide Y)

antibody name

ABOUT THIS ANTIBODY

NPY labels neurons that use neuropeptide Y as a neurotransmitter. This antibody labels the dorsal entopeduncular nucleus.

blahblahbla

Rabbit anti-NPY (Sigma, Cat#N9528, dilution 1:1000)

Select images

External Links:

 ZFIN:


LABELS THESE BRAIN STRUCTURES:

 dorsal entopeduncular nucleus,


KEY PUBLICATIONS

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30

Singh, C., Rihel, J., Prober, D.A. (2017)
Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling.
Current biology : CB. 27(24):3796-3811.e5.

synaptic vesicle 2 (SV2)

synaptic vesicle 2 (SV2)

Synaptic Vesicle 2 (SV2)

ABOUT THIS ANTIBODY

Synaptic Vesicle 2 labels synaptic neuropil.

We frequently use this antibody in conjunction with  anti-acetylated tubulin (IgG2b, Sigma), which labels beautifully the axonal connections, to aid anatomical orientation in transgenic specimens. In addition to these antibodies being informative from a neuroanatomical perspective, they are also invaluable as a tool for anatomical localisation. These antibodies can be used as a framework to easily compare the expression patterns of different transgenic lines and locate GFP-positive structure in the context of the brain. Both antibodies are mouse monoclonals but can be detected in the same specimen using subtype-specific secondary antibodies.

Mouse monoclonal anti-SV2 (IgG1) (DSHB, Cat#AB 2315387, dilution 1:500)

Select images

External Links:

 ZFIN:https://zfin.org/ZDB-ATB-081201-1


LABELS THESE BRAIN STRUCTURES:

Synaptic neuropil


KEY PUBLICATIONS

Buckley K, Kelly RB (1985)
Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells. 
J Cell Biol 100:1284–1294

Hendricks M, Jesuthasan S (2007)
Asymmetric innervation of the habenula in zebrafish. 
J Comp Neurol 502:611–619

Turner KJ1, Bracewell TG, Hawkins TA.
Anatomical dissection of zebrafish brain development.
Methods Mol Biol. 2014;1082:197-214. doi: 10.1007/978-1-62703-655-9_14.