ABOUT THIS ANTIBODY
anti-Parvalbumin labels .
Parvalbumin is a calcium-binding albumin protein found in brain and muscle. This antibody has relatively sparse labelling in the larval zebrafish brain. It nicely labels the olfactory epithelium, olfactory sensory nerve processes and the olfactory nerves, neuromasts of the lateral line system, amacrine cells in the retina and a subset of purkinje cells in the cerebellum and the pituitary. Parvalbumin also labels neurons in the optic tectum at adult stages (Robra et al., 2016).
Mouse monoclonal anti-Parvalbumin (IgG1) (Chemicon/Millipore, Cat#MAB1572, dilution 1:200)
All images by Monica Folgueira
LABELS THESE BRAIN STRUCTURES:
olfactory epithelium, olfactory nerve, olfctory bulb, amacrine cell in retina, pituitary, cerebellum, purkinje cell, optic tectum.
Yeo, J.Y., Lee, E.S., and Jeon, C.J. (2009)
Parvalbumin-immunoreactive neurons in the inner nuclear layer of zebrafish retina.
Experimental Eye Research. 88(3):553-560.
Kaslin, J., Kroehne, V., Benato, F., Argenton, F., and Brand, M. (2013)
Development and specification of cerebellar stem and progenitor cells in zebrafish: from embryo to adult. Neural Development. 8(1):9.
Kress, S., Biechl, D., Wullimann, M.F. (2015)
Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Brain structure & function. 220(4):1951-70.