Viewing entries tagged
telencephalon

Calretinin

Calretinin

Rabbit anti-calretinin

ABOUT THIS ANTIBODY

anti-calretinin labels neurons expressing the calcium binding protein calretinin.

Calcium-binding preoteins contribute to calcium homeostasis by buffering intercellular calcium. This antibody labels distinct groups of neurons in the central and peripheral nervous system.
 

Rabbit Polyclonal anti-calretinin (Swant, Cat#CR7697, dilution 1:1000)

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EXTERNAL LINKS:

 ZFIN:https://zfin.org/ZDB-ATB-160726-1
Swant: https://www.swant.com/pdfs/Rabbit_anti_calretinin_7697.pdf

LABELS THESE BRAIN STRUCTURES:

olfactory epithelium, olfactory glomeruli, dorsal telencephalon, ventral telencephalon, entopeduncular nucleus, preoptic area, preglomerular complex, mammillary body, hypothalamus (inferior lobe), torus lateralis, posterior tuberculum, thalamus(anterior thalamic nucleus), pretectum (parvocellular superficial pretectal nucleus), nucleus of the medial longitudinal fascicle. 

KEY PUBLICATIONS

Castro A1, Becerra M, Manso MJ, Anadón R.
Calretinin immunoreactivity in the brain of the zebrafish, Danio rerio: distribution and comparison with some neuropeptides and neurotransmitter-synthesizing enzymes. I. Olfactory organ and forebrain.
J Comp Neurol. 2006 Jan 20;494(3):435-59.

Beretta, C.A., Dross, N., Guglielmi, L., Bankhead, P., Soulika, M., Gutierrez-Triana, J.A., Paolini, A., Poggi, L., Falk, J., Ryu, S., Kapsimali, M., Engel, U., Carl, M. (2017)
Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting.
Current biology : CB. 27(2):270–278.

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits 10:30. https://doi.org/10.3389/fncir.2016.00030

Parvalbumin

Parvalbumin

Parvalbumin

ABOUT THIS ANTIBODY

anti-Parvalbumin labels .

Parvalbumin is a calcium-binding albumin protein found in brain and muscle. This antibody has relatively sparse labelling in the larval zebrafish brain. It nicely labels the olfactory epithelium, olfactory sensory nerve processes and the olfactory nerves, neuromasts of the lateral line system, amacrine cells in the retina and a subset of purkinje cells in the cerebellum and the pituitary. Parvalbumin also labels neurons in the optic tectum at adult stages (Robra et al., 2016).

Mouse monoclonal anti-Parvalbumin (IgG1) (Chemicon/Millipore, Cat#MAB1572, dilution 1:200)

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All images by Monica Folgueira

External Links:

 ZFIN

LABELS THESE BRAIN STRUCTURES:

olfactory epithelium, olfactory nerve, olfctory bulb, amacrine cell in retina, pituitary, cerebellum, purkinje cell, optic tectum.  

KEY PUBLICATIONS

 
Yeo, J.Y., Lee, E.S., and Jeon, C.J. (2009)
Parvalbumin-immunoreactive neurons in the inner nuclear layer of zebrafish retina.
Experimental Eye Research. 88(3):553-560.

Kaslin, J., Kroehne, V., Benato, F., Argenton, F., and Brand, M. (2013)
Development and specification of cerebellar stem and progenitor cells in zebrafish: from embryo to adult. Neural Development. 8(1):9.

Kress, S., Biechl, D., Wullimann, M.F. (2015)
Combinatorial analysis of calcium-binding proteins in larval and adult zebrafish primary olfactory system identifies differential olfactory bulb glomerular projection fields.
Brain structure & function. 220(4):1951-70.

Tyrosine Hydroxylase

Tyrosine Hydroxylase

Tyrosine Hydroxylase

ABOUT THIS ANTIBODY

Tyrosine Hydroxylase is an enzyme that catalyses the convertion of L-tyrosine to L-3,4-dihydroxyphenylalanine(L-DOPA) which is the precursor for the neurotransmitter dopamine. anti-TH labels dopaminergic neurons in the olfactory bulb, subpallium, preotic area, posterior tuberculum and locus coeruleus.

Rabbit polyclonal anti-TH (Chemicon/Millipore , Cat#AB152, dilution 1:1000)

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External Links:

 ZFIN

LABELS THESE BRAIN STRUCTURES:

 olfactory bulb, preoptic area, posterior tuberculum.

KEY PUBLICATIONS

 Yamamoto, K., Ruuskanen, J.O., Wullimann, M.F., and Vernier, P. (2010)
Two tyrosine hydroxylase genes in vertebrates: New dopaminergic territories revealed in the zebrafish brain. Molecular and Cellular Neuroscience. 43(4):394-402.

Yamamoto, K., Ruuskanen, J.O., Wullimann, M.F., and Vernier, P. (2011)
Differential expression of dopaminergic cell markers in the adult zebrafish forebrain.
The Journal of comparative neurology. 519(3):576-598.

Xi, Y., Yu, M., Godoy, R., Hatch, G., Poitras, L., and Ekker, M. (2011)
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Developmental dynamics. 240(11):2539-47.