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transgenic line

HuC/D

HuC/D

HuC/D

ABOUT THIS ANTIBODY

HuC/D is a pan-neuronal nuclear label and as such a very good counterstain for showing the structure of the brain.


anti-HuC/D (Manufacturer/cat#/dilution)

Select images by Chintan Trivedi imaged using a light-sheet microscope.

Flythrough movie of a 6dpf larval zebrafish brain labelled with anti-HuC and anti-HuD antibodies. Anti-HuC/D is a pan-neuronal nuclear label that labels all neurons in the nervous system.

Flythrough movie of a 6dpf larval zebrafish brain labelled with anti-HuC and anti-HuD antibodies. Anti-HuC/D is a pan-neuronal nuclear label that labels all neurons in the nervous system.

Montage of slices through the brain of a 6dpf zebrafish larvae labelled using anti-HuC/D antibody.

Montage of slices through the brain of a 6dpf zebrafish larvae labelled using anti-HuC/D antibody.

6dpf brain of a larval zebrafish labeled with anti-HuC/D. This stack was morphed to ZBB. This screenshot shows the HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this data using ZBB pleas…

6dpf brain of a larval zebrafish labeled with anti-HuC/D. This stack was morphed to ZBB. This screenshot shows the HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

External Links:

 ZFIN:

Download the HuC/D .png file to view the data using Zebrafish Brain Browser

LABELS THESE BRAIN STRUCTURES:

 pan-neuronal marker.

KEY PUBLICATIONS

NPY (neuropeptide Y)

NPY (neuropeptide Y)

NPY

ABOUT THIS ANTIBODY

NPY labels neurons that use neuropeptide Y as a neurotransmitter. This antibody labels the dorsal entopeduncular nucleus.

Rabbit anti-NPY (Sigma, Cat#N9528, dilution 1:1000)

Select images

External Links:

 ZFIN:

LABELS THESE BRAIN STRUCTURES:

 dorsal entopeduncular nucleus,

KEY PUBLICATIONS

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30

Singh, C., Rihel, J., Prober, D.A. (2017)
Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling.
Current biology : CB. 27(24):3796-3811.e5.

synaptic vesicle 2 (SV2)

synaptic vesicle 2 (SV2)

Synaptic Vesicle 2 (SV2)

ABOUT THIS ANTIBODY

Synaptic Vesicle 2 labels synaptic neuropil.

We frequently use this antibody in conjunction with  anti-acetylated tubulin (IgG2b, Sigma), which labels beautifully the axonal connections, to aid anatomical orientation in transgenic specimens. In addition to these antibodies being informative from a neuroanatomical perspective, they are also invaluable as a tool for anatomical localisation. These antibodies can be used as a framework to easily compare the expression patterns of different transgenic lines and locate GFP-positive structure in the context of the brain. Both antibodies are mouse monoclonals but can be detected in the same specimen using subtype-specific secondary antibodies.

Mouse monoclonal anti-SV2 (IgG1) (DSHB, Cat#AB 2315387, dilution 1:500)

Select images

External Links:

 ZFIN:https://zfin.org/ZDB-ATB-081201-1

LABELS THESE BRAIN STRUCTURES:

Synaptic neuropil

KEY PUBLICATIONS

Buckley K, Kelly RB (1985)
Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells. 
J Cell Biol 100:1284–1294

Hendricks M, Jesuthasan S (2007)
Asymmetric innervation of the habenula in zebrafish. 
J Comp Neurol 502:611–619

Turner KJ1, Bracewell TG, Hawkins TA.
Anatomical dissection of zebrafish brain development.
Methods Mol Biol. 2014;1082:197-214. doi: 10.1007/978-1-62703-655-9_14.