Viewing entries tagged
GABA

gad1a- FISH

August 8, 2020

About gad1a (glutamate decarboxylase 1a)

gad1a is orthologous to human GAD1 /GAD67(glutamate decarboxylase 1) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad1a is expressed in the nervous system in neurons that use GABA as a neurotransmitter.

Expression pattern

gad1a is expressed in GABAergic neurons throughout the nervous system.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probes against gad1a. This probe labels GABA-ergic neurons. — Flythrough movie of a 6dpf larvae labelled using fluorescent *in-situ hybridisation* using a probes against *gad1a*. This probe labels GABA-ergic neurons.

Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against gad1a. — Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent *in-situ hybridisation* using a probe against *gad1a*.

6dpf brain of a larval zebrafish labeled with a probe against gad1a FISH. this stack was morphed to ZBB. This screenshot shows the gad1a stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to … — 6dpf brain of a larval zebrafish labeled with a probe against *gad1a* FISH. this stack was morphed to ZBB. This screenshot shows the *gad1a* stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this *gad1a* data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi.

External links

ZFIN

Addgene probe link

Download the gad1a .png file to view the data using Zebrafish Brain Browser

FISH Protocol

Key Publications

Martin, S.C., Heinrich, G., and Sandell, J.H. (1998)
Sequence and expression of glutamic acid decarboxylase isoforms in the developing zebrafish.
The Journal of comparative neurology. 396:253-266.

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.

gad1b- FISH

July 7, 2020

About gad1B (glutamate decarboxylase 1b)

gad1b is orthologous to human GAD1 /GAD67(glutamate decarboxylase 1) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad1b is expressed in the nervous system in neurons that use GABA as a neurotransmitter.

Expression pattern

gad1b is expressed in GABAergic neurons throughout the nervous system.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probes against gad1b. This probe labels GABA-ergic neurons. — Flythrough movie of a 6dpf larvae labelled using fluorescent *in-situ hybridisation* using a probes against *gad1b*. This probe labels GABA-ergic neurons.

Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against gad1b. — Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent *in-situ hybridisation* using a probe against *gad1b.*

Z projection of gad1bexpression in a 6dpf zebrafish brain labelled with fluorescent in-situ hybridisation. — Z projection of *gad1b*expression in a 6dpf zebrafish brain labelled with fluorescent in-situ hybridisation.

6dpf brain of a larval zebrafish labeled with a probe against gad1b FISH. this stack was morphed to ZBB. This screenshot shows the gad1b stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to … — 6dpf brain of a larval zebrafish labeled with a probe against *gad1b* FISH. this stack was morphed to ZBB. This screenshot shows the *gad1b* stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this *gad1b* data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi.

External links

ZFIN

Addgene probe link

Download the gad1b .png file to view the data using Zebrafish Brain Browser

FISH Protocol

Key Publications

gad2- FISH

June 6, 2020

About gad2 (glutamate decarboxylase 2)

gad1b is orthologous to human GAD2/ GAD65(glutamate decarboxylase 2) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad2 is expressed in the nervous system in neurons that use GABA as a neurotransmitter.