gad1a- FISH

About gad1a (glutamate decarboxylase 1a)

gad1a is orthologous to human GAD1 /GAD67(glutamate decarboxylase 1) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad1a is expressed in the nervous system in neurons that use GABA as a neurotransmitter.

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Expression pattern

gad1a is expressed in GABAergic neurons throughout the nervous system.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probes against gad1a. This probe labels GABA-ergic neurons.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against gad1a.

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6dpf brain of a larval zebrafish labeled with a probe against gad1a FISH. this stack was morphed to ZBB. This screenshot shows the gad1a stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this gad1a data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi.

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External links

ZFIN

Addgene probe link

Download the gad1a .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Martin, S.C., Heinrich, G., and Sandell, J.H. (1998)
Sequence and expression of glutamic acid decarboxylase isoforms in the developing zebrafish.
The Journal of comparative neurology. 396:253-266.

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.

gad1b- FISH

About gad1B (glutamate decarboxylase 1b)

gad1b is orthologous to human GAD1 /GAD67(glutamate decarboxylase 1) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad1b is expressed in the nervous system in neurons that use GABA as a neurotransmitter.

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Expression pattern

gad1b is expressed in GABAergic neurons throughout the nervous system.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probes against gad1b. This probe labels GABA-ergic neurons.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against gad1b.

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Z projection of gad1bexpression in a 6dpf zebrafish brain labelled with fluorescent in-situ hybridisation.

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6dpf brain of a larval zebrafish labeled with a probe against gad1b FISH. this stack was morphed to ZBB. This screenshot shows the gad1b stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this gad1b data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi.

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External links

ZFIN

Addgene probe link

Download the gad1b .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Martin, S.C., Heinrich, G., and Sandell, J.H. (1998) 
Sequence and expression of glutamic acid decarboxylase isoforms in the developing zebrafish. 
The Journal of comparative neurology. 396:253-266.

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.

gad2- FISH

About gad2 (glutamate decarboxylase 2)

gad1b is orthologous to human GAD2/ GAD65(glutamate decarboxylase 2) this enzyme catalyses the decarboxylation of L-glutamic acid to make the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). gad2 is expressed in the nervous system in neurons that use GABA as a neurotransmitter.

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Expression pattern

gad2 is expressed in GABAergic neurons throughout the nervous system.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probes against gad2. This probe labels GABA-ergic neurons.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against gad2.

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Selected images

All imaging by Chintan Trivedi.

Z projection of gad2 expression in a 6dpf zebrafish brain labelled with fluorescent in-situ hybridisation.

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6dpf brain of a larval zebrafish labeled with a probe against gad2 FISH. this stack was morphed to ZBB. This screenshot shows the gad2 stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this gad2 data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

External links

ZFIN

Addgene probe link

Download the gad2 .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Martin, S.C., Heinrich, G., and Sandell, J.H. (1998) 
Sequence and expression of glutamic acid decarboxylase isoforms in the developing zebrafish. 
The Journal of comparative neurology. 396:253-266.

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.

glyt2a- FISH

About glyt2a (synonyms: slc6a5)

glycine transporter 2/ Solute Carrier Family 6 (Neurotransmitter Transporter), Member 5

glyt2a is orthologous to human GLYT2/ slc6a5 . This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This protein is found in glycinergic axons and glycinergic synapses. Glycine is a major inhibitory neurotransmitter in the central nervous system of vertebrates.

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Expression pattern

glyt2a is expressed in glycinergic neurons throughout the nervous system.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against glyt2a. This probe labels glycinergic neurons.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against glyt2a.

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Z projection of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against glyt2a. This probe labels glycinergic neurons.

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6dpf brain of a larval zebrafish labeled with a probe against glyt2a FISH. this stack was morphed to ZBB. This screenshot shows the glyt2a stack and a HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this glyt2a data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi on a lightsheet microscope.

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External links

ZFIN

Addgene probe link

Download the glyt2a .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.

Moly, P.K., Ikenaga, T., Kamihagi, C., Tariqul Islam, A.F., and Hatta, K. (2014)
Identification of initially appearing glycine-immunoreactive neurons in the embryonic zebrafish brain. Developmental Neurobiology. 74(6):616-32.

Higashijima, S.I., Schaefer, M., and Fetcho, J.R. (2004)
Neurotransmitter properties of spinal interneurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):19-37.

glyt2b- FISH

About glyt2b (synonyms: slc6a5)

glycine transporter 2/ Solute Carrier Family 6 (Neurotransmitter Transporter), Member 5

glyt2b is orthologous to human GLYT2/ slc6a5 . This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This protein is found in glycinergic axons and glycinergic synapses. Glycine is a major inhibitory neurotransmitter in the central nervous system of vertebrates.

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Expression pattern

glyt2b is expressed in glycinergic neurons in the hindbrain.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against glyt2b. This probe labels glycinergic neurons.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against glyt2b.

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Z projection of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against glyt2b. This probe labels glycinergic neurons.

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6dpf brain of a larval zebrafish labeled with a probe against glyt2b FISH. this stack was morphed to ZBB. This screenshot shows the glyt2b stack and a HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this glyt2b data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi on a lightsheet microscope.

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External links

ZFIN

Addgene probe link

Download the glyt2b .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.

Moly, P.K., Ikenaga, T., Kamihagi, C., Tariqul Islam, A.F., and Hatta, K. (2014)
Identification of initially appearing glycine-immunoreactive neurons in the embryonic zebrafish brain. Developmental Neurobiology. 74(6):616-32.

Higashijima, S.I., Schaefer, M., and Fetcho, J.R. (2004)
Neurotransmitter properties of spinal interneurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):19-37.

sst1.1- FISH

About sst1.1

somatostatin 1

sst1 is orthologous to human SST, somatostatin . This gene encodes a peptide hormone that modulates neurotransmission. Zebrafish have 6 different somatostatin genes.

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Expression pattern

sst1.1 is expressed in the habenula, neurosecretary pre-optic area NPO, subpallium, preoptic area, posterior tuberculum, ventral thalamus, hypothalamus, interpeduncular nucleus, medulla oblongata.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against sst1.1. This probe labels neurons that secrete the peptide hormone somatostatin.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against sst1.1.

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Z projection of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against sst1.1.

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6dpf brain of a larval zebrafish labeled with a probe against sst1.1 . this stack was morphed to ZBB. This screenshot shows the sst1.1 stack and a HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi on a lightsheet microscope.

dHbM: medial subnucleus of the dorsal habenular.

NPO: neurosecretary preoptic area

SubP; subpallium: PO; preoptic area: IPN; interpeduncular nucleus.

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External links

ZFIN

Addgene probe link

Download the sst1.1 .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Devos, N., Deflorian, G., Biemar, F., Bortolussi, M., Martial, J.A., Peers, B., and Argenton, F. (2002)
Differential expression of two somatostatin genes during zebrafish embryonic development.
Mechanisms of Development. 115(1-2):133-137.

deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E. (2014) Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish.
Genesis (New York, N.Y. : 2000). 52(6):636-55.

Liu, Y., Lu, D., Zhang, Y., Li, S., Liu, X., and Lin, H. (2010)
The evolution of somatostatin in vertebrates.
Gene. 463(1-2):21-28.

Herget, U., Wolf, A., Wullimann, M.F., and Ryu, S. (2014)
Molecular neuroanatomy and chemoarchitecture of the neurosecretory preoptic-hypothalamic area in zebrafish larvae.
The Journal of comparative neurology. 522(7):1542-64.

Herget, U., Ryu, S. (2015)
Coexpression analysis of nine neuropeptides in the neurosecretory preoptic area of larval zebrafish.
Frontiers in Neuroanatomy. 9:2.

tac3a- FISH

About tac3a

tachykinin precursor 3a
synonyms: Neurokinin B

tac3a is orthologous to human TAC3 and in rodents to tac2. TAC3/Neurokinin B may participate in neuroendocrine control of zebrafish reproduction by stimulating gonadotropin-releasing hormone (GnRH) neurons, mainly via kisspeptin. In mammals, NeurokininB is coexpressed with kisspeptin (Kiss) and dynorphin in neurons of the arcuate nucleus of the hypothalamus, which are therefore called KNDy neurons

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Expression pattern

At embryonic and larval stages tac3a is expressed asymmetrically in the dorsal habenula. tac3a mRNA-expressing cells detected in the subpallium (SubP), posterior tuberculum(PT) , rostral and intermediate hypothalamus, posterior tuberal nucleus (PTN), tegmentum(Teg), superior raphe (SR) and the medulla oblongata.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against tac3a.

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Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a probe against tac3a.

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Z projection of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a probe against tac3a.

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6dpf brain of a larval zebrafish labeled with a probe against tac3a . this stack was morphed to ZBB. This screenshot shows the tac3a stack and a HuC/D antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

Selected images

All imaging by Chintan Trivedi on a lightsheet microscope.

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External links

ZFIN

Addgene probe link

Download the tac3a .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Key Publications

Zhou, W., Li, S., Liu, Y., Qi, X., Chen, H., Cheng, C.H., Liu, X., Zhang, Y., and Lin, H. (2012)
The evolution of tachykinin/tachykinin receptor (TAC/TACR) in vertebrates and molecular identification of the TAC3/TACR3 system in zebrafish (Danio rerio).
Molecular and Cellular Endocrinology. 361(1-2):202-212.

deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E. (2014) Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish.
Genesis (New York, N.Y. : 2000). 52(6):636-55.

Pandey, S., Shekhar, K., Regev, A., Schier, A.F. (2018)
Comprehensive Identification and Spatial Mapping of Habenular Neuronal Types Using Single-Cell RNA-Seq. Current biology : CB. 28(7):1052-1065.e7.

Ogawa, S., Ramadasan, P.N., Goschorska, M., Anantharajah, A., Ng, K.W., and Parhar, I.S. (2012)
Cloning and expression of tachykinins and their association with kisspeptins in the brains of zebrafish.
The Journal of comparative neurology. 520(13):2991-3012.

Biran, J., Palevitch, O., Ben-Dor, S., and Levavi-Sivan, B. (2012)
Neurokinin Bs and neurokinin B receptors in zebrafish-potential role in controlling fish reproduction. Proceedings of the National Academy of Sciences of the United States of America. 109(26):10269-10274.

vglut2/slc17A6 - FISH

About vglut2

Synonyms: slc17A6 (solute carrier family 17 member 6), VGLUT2.2. vglut2a, vglut2b.

vglut2 encodes a vesicular glutamate transporter. Due to genome duplication in zebrafish two copies of the vglut2 or slc17A6 exist in zebrafish. The probe used for this FISH is a cocktail that contains probes specific to both the vglut2a and vglut2b genes.

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Expression pattern

vglut2 is expressed in excitatory glutamatergic neurons. The expression pattern of this gene is very widespread, neurons in all brain regions express vglut2.

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Selected images

All FISH and imaging done by Chintan Trivedi using a lightsheet microscope.

Flythrough movie of a 6dpf larvae labelled using fluorescent in-situ hybridisation using a cocktail of probes against vglut2. This probe labels glutamatergic neurons.

View fullsize

Montage of slices through the brain of a 6dpf zebrafish larvae labelled using fluorescent in-situ hybridisation using a cocktail of probes against vglut2.

6dpf brain of a larval zebrafish labeled with a cocktail probe against vglut2 FISH. this stack was morphed to ZBB. This screenshot shows the vglut2 stack and a HuCD antibody labelling uploaded to ZBB and viewed using their online browser. If you would like to look at this vglut data using ZBB please download the .png file using the link below and upload it to ZBB using the Lines> Custom>Load option.

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External links

ZFIN vglut2a
ZFIN vglut2b

Addgene probe link

Download the vglut2 .png file to view the data using Zebrafish Brain Browser

FISH Protocol

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Publications

Higashijima, S.I., Mandel, G., and Fetcho, J.R. (2004)
Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
The Journal of comparative neurology. 480(1):1-18.