by Tom Hawkins & Kate Turner

Forebrain (purple), mid-brain (blue) and hindbrain (green).

by Gaia Gestri

by Tom Hawkins

Sloth mutant and wildtype embryos arranged so to resemble a muscle sarcomere

by Kate Turner

Lateral view of a Brn3a:GFP embryo labelled wth anti-GFP and anti-tubulin antibodies. This transgenic line labels the sensory neuromasts, retina , optic tectum and the habenula. 

by Mate Varga

by Kate Turner

Ventral view of a Brn3a:GFP embryo labelled wth anti-GFP and anti-tubulin antibodies. This transgenic line labels the sensory neuromasts, the habenula, retina and optic nerves decussating at the optic chiasm on their way to the optic tectum.  

by Kate Turner

Calretinin positive nuclei (green) and their processes in the olfactory bulbs, epithalamus, optic tectum and cerebellum. Dorsal 3dpf embryo.

by Kate Turner

Ventral view of a 4dpf larvae labelled with anti-5HT antibody to label serotinergic neurons in the preoptic, posterior tuberculum, hypothalamus and raphe.

by Kate Turner

Calretinin positive nuclei and their processes in the optic tectum and optic nerve (green). Frontal view of 3dpf dissected embryo.

by Kate Turner

GFP expressed in many dopaminergic and GABAergic cells within the brain, shown here in the optic tectum, cerebellum, epithalamus and telencephalon of Tg(Dlx4/6:GFP) embryo. Dorsal view, 3dpf.

by Kate Turner

Pineal organ and the pineal processes that form the dorso-ventral diencephalic tract (green) descending ventrally in 60hpf Tg(FoxD3:GFP) embryo.

by Kate Turner

Enhancer-trap transgenic expressing GFP in the tela choroidia, pineal organ and olfactory bulbs. Dorsal view, 60 hpf.

by Jay Patel

Dorsal view of a 4dpf larva labelled with anti-tubulin(green) and anti-SV2(red) antibodies.

by Kate Turner

Dorsal view of a 4dpf Tg(Brn3a:GFP)embryo.

by Kate Turner

Lateral view of a 4dpf Tg(Brn3a:GFP) embryo.

by Dave Lyons

by Monica Folgueira

by Kate Turner

Dorsal view of a 4dpf Tg(ET11:GFP) embryo.

Kate Turner

by Kate Turner

3D rendering of a 4dpf Tg(ET16:GFP) embryo.

by Kate Turner

Ventral view of a 4dpf Tg(vmat2:GFP) embryo. This enhancer-trap transgenic labels a subset of neurons in the olfactory bulbs and telencephalon, hypothalamus and posterior tuberculum and the raphe. 

by Isaac Bianco

by Kate Turner

by Kate Turner

This transgenic shows habenula terminals spiralling in the IPN.

by Isaac Bianco

by Florencia Cavodeassi

Section through an eye of a 3 days old embryo immunostained to highlight a subset of neurons in the retina (green, Tg{HuC:GFP}) and their axonal projections (red, Zn5).

by Florencia Cavodeassi

Coronal section through the head of a 2.5 days old embryo at the level of the eyes, stained with toloudine blue to reveal the highly organised architecture of the differentiating retina

by Florencia Cavodeassi

Dorsal view of the anterior neural plate, immunostained to reveal the eye field, the primordium of the eyes (red, Tg{rx3:GFP}) and the distribution of filamentus actin (green, phalloidin).

by Florencia Cavodeassi

Frontal view of the anterior neural plate at the onset of evagination of the optic vesicles (red, Tg{rx3:GFP}; green, F-actin).

by Isaac Bianco

By Kara Cerveny

This photomicrograph shows the retina from the eye of a three-day-old zebrafish (Danio rerio).

The retina is viewed here from the front, as if the viewer is looking directly into the eye of the fish. This image is of the whole eye, created by reflecting half the image to represent the naturally occurring symmetry. It was created using a double in situ hybridisation - a staining technique that identifies spatial expression of gene products. Using this technique, different structures can be identified by staining for genes known to be found in specific tissues. 

Retinal stem cells start to differentiate to become functional retinal neurons that are responsible for sending visual signals to the brain. Undifferentiated stem cells are shown in red, whereas the cells that have started to differentiate are shown in purple and are located at the periphery of the retina. The central yellow region is the lens.
 

by Kate Turner

by Kate Turner

by Kate Turner

Lateral view of a Tg(tbr:GFP) and tubulin. GFP labelling cells in the telencephalon, midbrain and hindbrain, tubulin (red) labelling the axonal tracts in the brain.

by Lukas Roth

by Lukas Roth

by Lukas Roth

by Lukas Roth

Dorsal views of diI and diO labelled retinal projections in a wingnut mutant

by Miguel Concha

Dorsal view of the pineal organ and left-sided parapineal nucleus in a fish embryo1

by Miguel Concha

by Miguel Concha

by Miguel Concha

The larger pineal organ in green (centre) with the left sided smaller parapineal nucleus. The bilateral asymetric (larger in the left) habenulae.

by Miguel Concha

By Monica Folgueira

Confocal micrograph of a transgenic zebrafish embryo at 24 hours post-fertilization, showing expression of the fusion protein PARD3_GFP. This means that whenever the pard3 gene is expressed green fluorescent protein (GFP) will be produced, allowing the researcher to visualise the location of this gene in development. 

In this image the embryo is viewed from the back (dorsally) sitting on top of a big yolk sac that provides nutrients for its development. GFP expression is seen in the surface of the neuroepithelium and thus highlights the ventricle of the brain, as well as in epithelial cells of the skin recovering the yolk.

By Monica Folgueira

Confocal micrograph of a transverse section of the brain of a zebrafish embryo, at the level of the hindbrain. The brain section was stained using an antibody that marks a calcium binding protein (calretinin). The image shows a big reticulospinal neuron and its processes, surrounded by other smaller neurons. Reticulospinal neurons are specific neurons found in the brains of fish that are thought to be involved in coordinating swimming.

by Monica Folgueira

by Monica Folgueira

by Marina Mione

by Marina Mione

by Masaya Take-Uchi

by Masaya Take-Uchi

by Anon

by Jenny Regan

By Steve Wilson and Rachel MacDonald

This differential interference contrast image
shows a section through the eye of a three-day old
zebrafish embryo. At this stage of development,
the nerve cells in the retina have begun to
separate into different layers. The outer layer of
cells are stained black with a marker for
photoreceptor cells, the neurons that respond to
light. (credit: R. MacDonald & S. Wilson)

By Rachel MacDonald and Steve Wilson

Light microscope image of a WT(left) and mutant(right) two-day-old zebrafish embryo which has been stained to show
all of the nerve cell processes in the eyes and
brain. This embryo has a mutation in the Noi gene
causing many of the processes (axons) to get lost
as they cross the midline from the eye to their
target brain region.

By Rachel MacDonald and Steve Wilson

This differential interference contrast image
shows a section through the eye of a three-day old
zebrafish embryo. At this stage of development,
the nerve cells in the retina have begun to
separate into different layers. The outer layer of
cells are stained black with a marker for
photoreceptor cells, the neurons that respond to
light.

by Silvia Castro

by Gaia Gestri

by Leonardo Valdivia

by Kate Turner

by Jen Cook

by Jen Cook

by Jen Cook

by Keith Wilson

by Steve Wilson

By Steve Wilson

Lateral view of the head region in a zebrafish
embryo which shows neuronal structure in the
epiphysial region of the diencephalon, part of
the developing forebrain. This is the region where
where neurons first develop in the forebrain.
Here, axons labelled with diI can be seen
extending from epiphysial projection neurons.

By Steve Wilson

This zebrafish embryo neuron is extending a long
process or axon through the brain, whose leading
edge is called the growth cone. The growth cone
explores the environment in order to navigate
towards the correct target nerve cells where it
will eventually form a synapse, a connection
through which nerve impulses pass from one neuron
to the next.

By Steve Wilson

Neurons extending numerous axons in the developing
forebrain of a zebrafish embryo.

by Zsolt Lele

by Aisling O'Sullivan & Marcus Ghosh

The expression pattern of Galanin mRNA (by fluorescent in-situ hybridisation), at 6dpf, in 7 different WT fish. To make the samples comparable they have been registered using tERK.

by Gaia Gestri

Sagittal section across an 8 days post-fertilization zebrafish eye with the outer photoreceptor segments stained by anti-zpr1 (blue), UV cones expressing GFP (Tg(opn1sw1:GFP), magenta) and nuclei counterstained with DAPI (grey).

by Gaia Gestri

Sagittal section across an 8 days post-fertilization zebrafish eye with the outer photoreceptor segments stained by anti-zpr1 (blue), UV cones expressing GFP (Tg(opn1sw1:GFP), green) and nuclei counterstained with DAPI (magenta).

by Gaia Gestri

Pseudocoloured composite image of a zebrafish eye expressing the UV cones transgenic line Tg(opn1sw1:GFP) and counterstained with anti-zpr1 (outer photoreceptor segments) and DAPI (cell nuclei).

by Gaia Gestri

Sagittal section across a zebrafish eye with the outer photoreceptor segments stained by anti-zpr1 (magenta), amacrine and retinal ganglion cells expressing GFP (Tg(islt1:GFP)), and counterstained with DAPI (blue).