Image by Xhuljana Durmishi

Image by Aanandita Korthurkar

By Xhuljana Durmishi

Image by Gregory Patient

A three round IBEX on a five days post fertilisation zebrafish retinal section, showing nuclei, different photoreceptor components, Müller glia, microglia, amacrine and retinal ganglion cells. The markers are: green – Glutamate Synthetase, red – GNAT2, cyan – M/L opsin, white – HuC/D, yellow – PNA lectin, magenta – LCP1, blue – DAPI.

Image by Manuela Lahne

Image by Nicole Noel

Section of cell body of killifish retina

Image by Elisabeth Kugler

Müller glia express specific proteins that allow them to carry out their support functions in the retina. Cryosection of the zebrafish retina and immunohistochemistry for glutamine synthetase (magenta).

Image by Ryan MacDonald

By Xhuljana Durmishi

Image by Elisabeth Kugler

False-colour illustration of zebrafish retina neurons (blue) and glia progenitors (yellow) at 48 hours post fertilization, acquired with Zeiss AiryScan microscopy. Image processing was conducted with Fiji software.

Image by Elisabeth Kugler

Müller glia are specifically labelled in the transgenic background Tg(gfap:gfp). We can use transgenic zebrafish or antibodies to specifically label and identify the glia or neurons in the retina. Cryosection of the zebrafish retina and immunohistochemistry for GFP.

Image by Natalia Jaroszynska

Müller glia (green) interact with all of the surrounding retinal cells in different retinal layers, including horizontal and amacrine cells (magenta). Zebrafish retina at 3 days post fertilisation.

By Elisabeth Kugler

As the zebrafish retina matures glial cells must support the tissue to maintain healthy neurons and their connections or synapses. Cryosection of the adult zebrafish retina and staining with DAPI (blue), phalloidin (red), zpr3 (photoreceptors; magenta) and ribeye (synapses; green).

Image by Elisabeth Kugler

The retina connects to the visual centres in the brain. Cryosection of the zebrafish brain and staining with DAPI (blue), phalloidin (red), HuC/D (neurons; magenta) and GFP (green). Genotype is Tg(NBT-GCaMP3) which labels neurons in the retina and tectum.

Image by Nicole Noel

Immunohistochemistry on 12 wpf killifish retina section. Magenta is zpr-1 and DAPI is in cyan.

Image by Elisabeth Kugler

We are trying to push the limits of our imaging to understand glial cells. This is a semi-super resolution reconstruction of Müller glia cells (green) and two different classes of amacrine cells (magenta and red) that will come together to make distinct synapses in the inner plexiform layer. Cryosection of the zebrafish retina taken on a Leica Sp8 and processed with Lightning software.

Image by Natalia Jaroszynska

Image by Ryan MacDonald

False-colour illustration of zebrafish retina Muller glia cells at 4dpf; same image reproduced with different look up tables. Image processing was conducted with Fiji software.

Image by Xhuljana Durmishi

Image by Nicole Noel

Section of killifish retina zpr-3 in magenta

Image by Elisabeth Kugler

Image shows multi-colour false segmentation of the zebrafish retina at 3dpf. Image processing was conducted with Fiji software.

Image by Elisabeth Kugler

False-colour illustration of zebrafish retina neurons (blue) and glia progenitors (yellow) at 48 hours post fertilization, acquired with Zeiss AiryScan microscopy. Image processing was conducted with Fiji software.

Image by Natalia Jaroszynska

Immunostaining on 5 day old coronal zebrafish cryosections. Amacrine and retinal ganglion cells labelled with HuC/D (magenta) and bipolar cells labelled with PKC (red). Nuclei labelled with DAPI.

Image by Gregory Patient

A multiplex of four rabbit antibodies on the same five days post fertilisation zebrafish retinal section using micro-conjugation kits. Photoreceptors shown in green by GNAT2, Müller glia cells in magenta with RLBP1 and synaptic terminals shown in yellow by PKCβ and in cyan with Ribeye.

Image by Elisabeth Kugler

Zebrafish retinal synapses (magenta) and Muller glia (green) at 120 hours post fertilisation, acquired with Zeiss AiryScan microscopy. Image processing was conducted with Fiji software.

Image by Manuela Lahne

Image by Elisabeth Kugler

Image by Elisabeth Kugler

Image by Elisabeth Kugler

Image by Elisabeth Kugler

Image by Aanandita Korthurkar

Image by Eva-Maria Breitenbach

It shows the the image of a 2 mpf female killifish with anti-Gs staining which labels Müller glia cells.

Image by Isabel Bravo

Image by Nicole Noel

Section of killifish retina zpr-1 in magenta and DAPI in cyan

By Xhuljana Durmishi

By Xhuljana Durmishi

Image by Natalia Jaroszynska

Image by Natalia Jaroszynska

Image by Natalia Jaroszynska

Image by Natalia Jaroszynska

Image by Gregory Patient

Immunohistochemistry on a five days post fertilisation zebrafish retinal section, showing Müller glia, amacrine, retinal ganglion cells and F-Actin. The markers are: green – Glutamate Synthetase, cyan – HuC/D, magenta – Phalloidin.

Image by Natalia Jaroszynska

Developing Müller glia (white) and their actin cytoskeleton (magenta) in a 2.5day old zebrafish retina.

Image by Elisabeth Kugler

The inner plexiform layer is the major synaptic neurpil of the retina. In this layer Müller glia cell (multi-coloured) sends elaborate projections to contact and support neuronal synapses. Genotype Tg(Tp1:Venus) and false coloured in FIJI.

Image by Manuela Lahne

Image by Elisabeth Kugler

In the mature retina Müller glia cells “tile” to make an elaborate non-overlapping support network for neurons. Each Müller glia cell is morphologically specialised at each layer of the retina and will establish its’ own unique spatial domain during development. Optical section through the intact zebrafish retina with a Zeiss Airyscan confocal. Genotype Tg(Tp1:Venus) and false coloured in FIJI.

Image by Aanandita Korthurkar

Image by Ryan MacDonald

The star of the show: In the retina the principal glial type are the Müller glia (blue). These cells are elaborately shaped to contact neurons (yellow) and provide them with support so they function properly. I want to know how these glial cells get their shape in development. Optical section through the intact zebrafish retina with a Zeiss airyscan confocal. Genotype Tg(Tp1:Venus;ptf1a:dsRED).

Image by Nicole Noel

In situ hybridization chain reaction on killifish retina. Showing photoreceptors.

Image by Aanandita Korthurkar