In the retina the principal glial type are the Müller glia (blue). These cells are elaborately shaped to contact neurons (yellow) and provide them with support so they function properly. I want to know how these glial cells get their shape in development. Optical section through the intact zebrafish retina with a Zeiss airyscan confocal. Genotype Tg(Tp1:Venus;ptf1a:dsRED).
Glial cells support healthy neurons
As the zebrafish retina matures glial cells must support the tissue to maintain healthy neurons and their connections or synapses.Cryosection of the adult zebrafish retina and staining with DAPI (blue), phalloidin (red), zpr3 (photoreceptors; magenta) and ribeye (synapses; green).
Müller glia cells
In the mature retinaMüller glia cells “tile” to make an elaborate non-overlapping support network for neurons. Each Müller glia cell is morphologically specialised at each layer of the retina and will establish its’ own unique spatial domain during development. Optical section through the intact zebrafish retina with a Zeiss Airyscan confocal. Genotype Tg(Tp1:Venus) and false coloured in FIJI.
The inner plexiform layer
The inner plexiform layer is the major synaptic neurpil of the retina. In this layer Müller glia cell (multi-coloured) sends elaborate projections to contact and support neuronal synapses. Genotype Tg(Tp1:Venus) and false coloured in FIJI.
The retina connects to the visual centres in the brain
The retina connects to the visual centres in the brain. Cryosection of the zebrafish brain and staining with DAPI (blue), phalloidin (red), HuC/D (neurons; magenta) and GFP (green). Genotype is Tg(NBT-GCaMP3) whichlabels neurons in the retina and tectum.
Müller glia express specific proteins that allow them to carry out their support functions in the retina. Cryosection of the zebrafish retina and immunohistochemistry for glutamine synthetase (magenta).
semi-super resolution reconstruction of Müller glia cells
We are trying to push the limits of our imaging to understand glial cells. This is a semi-super resolution reconstruction of Müller glia cells (green) and two different classes of amacrine cells (magenta and red) that will come together to make distinct synapses in the inner plexiform layer. Cryosection of the zebrafish
Müller glia are specifically labelled in the transgenic background Tg(gfap:gfp). We can use transgenic zebrafish or antibodies to specifically label and identify the glia or neurons in the retina. Cryosection of the zebrafish retina and immunohistochemistry for GFP.