How can you tell if a 2-photon microscope is performing at its best? Do the optics need aligning or should you install a new PMT? Our new paper in Nature Protocols provides several step-by-step procedures (no special equipment necessary) to test different aspects of microscope performance that can help troubleshoot issues and ensure consistent results over time (and across different labs).
Check out the paper here: https://www.nature.com/articles/s41596-024-01120-w
Working on this paper was a really fun group-effort. Congratulations to all the co-authors!