MÜLLER GLIA
Müller cells are radial glia that perform a wide variety of support functions for retinal neurons. Immunolabeling for carbonic anhydrase or glutamine synthetase shows that the Müller cell bodies are positioned in a row in the middle of the inner nuclear layer. Müller glial processes, which are positive for the glial fibrillary acidic protein (GFAP), extend radially across the thickness of the retina and compose parts of the outer and inner limiting membranes. The outer limiting membrane is formed by the tight junctions that connect Müller glia (MG) apical processes and photoreceptors cell bodies while the inner limiting membrane contains the MG end feet.
Schematic depicting the different cell types and layers that make up the neural retina in an adult zebrafish adapted from Baden et al., 2019. Müller glia have their cell bodies in the inner nuclear layer and span the retina with their end feet forming the inner and outer limiting membranes. They elaborate processes within both the inner and outer plexiform layers.