Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

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Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 

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More images

Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green), SV2(cyan) and tubulin(red) antibodies.

by Monica Folgueira

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP and anti-tubulin(cyan).

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP.

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green).

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and anti-tubulin antibodies.

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and tubulin(cyan) antibodies.

by Kate Turner

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

by KateTurner

Olfactory glomeruli in Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

Frontal view showing the GFP +processes of olfactory bulb interneurons innervating the glomeruli.

Monica Folgueira

24hpf dorsal view of dlx:GFP with tubulin

Tubulin shows the longitudinal axon scaffold formed by this early stage. Several clusters of dlx:GFP + cells are present in the telencephalon, diencephalon, midbrain and hindbrain.

Monica Folgueira

Olfactory bulb Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

single section through a 4dpf olfactory bulb.

Monica Folgueira

3dpf frontal view of dlx:GFP larvae

Monica Folgueira

3dpf dorsal view dlx:GFP

Showing many GFP+ neurons in the optic tectum and cerebellum.

Monica Folgueira

2dpf dlx:GFP ventral view

Shows cells throughout the subpallium and preoptic area and hypothalamic lobes.

Monica Folgueira

3dpf lateral view of dlx:GFP in forebrain

Monica Folgueira

3dpf late view of dlx:GFP hindbrain

showing dense cluster of GFP+ cells in cerebellum and scattered cells in the spinal cord.

Monica Folgueira

3dpf dorsal view of dlx:GFP hindbrain

Monica Folgueira

3dpf frontal section of dlx:GFP subpallium

Monica Folgueira

3dpf thick section of dlx:GFP through midbrain/diencephalon

Shows tectal and pretectal expression.

Monica Folgueira

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab

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Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

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Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.

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Et(fos:Gal4-VP16)s1026t 4dpf dorsal

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker TOTO-3(grey).

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

Single z-slice through the eye.

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf ventral

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

single z-slice at level of subpallium

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at level of preglomerular complex and hypothlamus.

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice through the posterior tuberculum

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the prethalmus and thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the ventral habenulae

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the dorsal habenulae and pineal organ.

External Links:

 ZFIN

Lab or Origin:

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Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.

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Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Tg(lhx5:GFP)b1205

About

 This transgenic line recapitulates the expression of lhx5 transcription factor. Generated by the Westerfield lab using BAC transgenesis. A kaede version of this line is also available. This transgenic has been used to study pathfinding in early telencephalic development (Guo et al., 2012; Zhang et al., 2012, Turner et al., 2019) and also to look at areas afferent to the habenula at later stages of development (Turner et al., 2016).

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Tg(lhx5:GFP) 4dpf lateral view

GFP+ populations in the forebrain innervate all habeunula subnuclei.

Abbreviations: Dp; posterior area of the pallium; Hb, habenula; OB, olfactory bulb; PO, preoptic area; PTh, prethalamus; tHC, tract of the habenula commissure; StMed, stria medularis; vENT, ventral entopeduncular nucleus.

Figure taken from Turner et al., 2016.

Tg(lhx5:GFP) lateral view 48hpf.

GFP+ populations in the forebrain innervate all habeunula subnuclei.

Abbreviations: Dp; posterior area of the pallium; Hb, habenula; OB, olfactory bulb; PO, preoptic area; PTh, prethalamus; tHC, tract of the habenula commissure; StMed, stria medularis; vENT, ventral entopeduncular nucleus.

Figure taken from Turner et al., 2016.

Dorsal view of the habenula in a 4dpf Tg(lhx5:GFP) larva.

All habenula subnuclei are innervated by GFP+ processes in the Tg(lhx5:GFP) line.

Abbreviations: dHbl, lateral subnucleus of the dorsal habeula; dHbm, medial subnucleus of the dorsal habenula; vHb, ventral subnucleus of the habenula.

lateral view of an adult Tg(lhx5:GFP) zebrafish

the ventral entopeduncular nucleus(vENT) is strongly labelled in this transgenic line.

Abbreviations: ac, anterior commissure; Pal, pallium; OB, olfactory bulb; vENT, ventral entopeduncular nucleus.

Image from Turner et al., 2016.

transverse section through the telencephalon of a Tg(lhx5:GFP) zebrafish.

the ventral entopeduncular nucleus(vENT) is strongly labelled in this transgenic line.

Abbreviations: ac, anterior commissure; dENT, dorsal entopeduncular nucleus; Dp, posterior area of the pallium; vENT, ventral entopeduncular nucleus.

Image from Turner et al., 2016.

transverse section through the habenula of an adult Tg(lhx5:GFP) zebrafish

All habenula subnuclei are innervated by GFP+ processes in the Tg(lhx5:GFP) line.

Image from Turner et al., 2016

Tg(lhx5:GFP)(cyan) 4dpf dorsal view with SV2(magenta)

Tg(lhx5:GFP)(cyan) 4dpf ventral view with SV2(magenta)

Tg(lhx5:GFP)(cyan) 4dpf lateral view

Tg(lhx5:GFP)(cyan) 4dpf lateral view with SV2(magenta)

External Links:

 ZFIN

Lab or Origin: Westerfield Lab

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Expressed in: 

 olfactory bulb, pallium, ventral entopeduncular nucleus, preoptic area, prethalamus, stria medularis, tract of the habenula commissure, habenula commisure, habenula neuropil.

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Key Publications

Gao, J., Zhang, C., Yang, B., Sun, L., Zhang, C., Westerfield, M., and Peng, G. (2012)
Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish.
PLoS One. 7(5):e36516.

Zhang, C., Gao, J., Zhang, H., Sun, L., and Peng, G. (2012)
Robo2-Slit and Dcc-Netrin1 Coordinate Neuron Axonal Pathfinding within the Embryonic Axon Tracts.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(36):12589-12602.

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30.

Turner, K.J., Hoyle, J., Valdivia, L.E., Cerveny, K.L., Hart, W., Mangoli, M., Geisler, R., Rees, M., Houart, C., Poole, R.J., Wilson, S.W., Gestri, G. (2019)
Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits.
PLoS One. 14:e0211073.

Tg(-2.7shh:GFP)

About

The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish  two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.

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All images of shh:GFP were produced by Monica Folgueira.

4dpf lateral shh:GFP with acetylated tubulin(cyan) and sytox(grey)

pHb, habenula; lfb, lateral forebrain bundle; OB, olfactory bulb; OT, optic tectum; ot, optic tract; Pal, pallium; PT, posterior tuberculum; PTh, prethalamus; Th, thalamus; ZLI, zona-limitans intrathalamica.

shh:GFP, tubulin and sytox 4dpf lateral forebrain

shh:GFP and tubulin. 4 dpf lateral forebrain

shh:GFP and tubulin. 4dpf dorsal view

single z-slice through telencephalon

shh:GFP, tublin and sytox at 4 dpf showing GFP + processes innervating the telencephalon.

shh:GFP dorsal view of 4dpf larvae

Frontal transverse z-slice through the telencephalon of shh:GFP

shh:GFP, tublin and sytox at 4.5dpf showing GFP + processes innervating the telencephalon.

shh:GFP and tubulin, 4dpf lateral view

shh:GFP lateral view 4dpf

shh:GFP, tubulin 4dpf ventral view

Shows expression in floor plate, posterior tuberculum and hypothalamus

shh:GFP and tubulin 24hpf lateral view

shh:GFP lateral forebrain at 24hpf

Shows expression in floor plate and zona limitans intrathalamica.

shh:GFP and tubulin lateral view of 24hpf

External Links:

 ZFIN

Lab or Origin: Nüsslein-Volhard, Christiane

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Expressed in: 

basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.

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Key Publications

 Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.

Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176

Tg(slc17a6b: DsRed)nns9Tg

About

 Slc17a6b is a vesicular glutamate transporter that that mediates the uptake of the excitatory neurotransmitter glutamate into vesicles in the presynaptic terminals of excitatory neurons. This BAC transgenic line from the Yoshihara lab drives the expression of DSRed in glutamatergic neurons. Expression can be seen in many neurons throughout the brain. Strong expression in the dorsal and ventral habenular subnuclei, olfactory bulbs, the pallium and optic tectum

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Tg(slc17a6b:DsRed), tubulin and SV2 24hpf frontal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin and SV2 hpf dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin and SV2 48hpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 48hpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green

Tg(slc17a6b:DsRed) 48hpf Lateral

Tg(slc17a6b:DsRed), tubulin, SV2 48hpf Dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed) 48hpf Dorsal

Tg(slc17a6b:DsRed), tubulin 4dpf Dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green;

Tg(slc17a6b:DsRed) 4dpf Dorsal

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed) 4dpf Lateral

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed) and tubulin 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed) 4dpf Lateral

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Ventral

g(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 4dpf Ventral

g(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Ventral

External Links:

 ZFIN

Lab or Origin: Yoshihara Lab

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Expressed in: 

 glutamatergic neurons, olfactory epithelium, olfactory bulb, pallium, subpallium, dorsal habenula, ventral habenula, preoptic area, prethalamus, pretectum (AF9), optic tectum, trigeminal sensory ganglion.

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Key Publications

 
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009)
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish.
The Journal of neuroscience. 29(15):4756-4767.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum.
Developmental Biology. 343(1-2):1-17.