anti-acetylated tubulin

ABOUT THIS ANTIBODY

anti-acetylated tubulin labels axonal tracts. Please see our separate white matter section for more information about individual tracts and commissures. 

This is a beautiful antibody and like anti-SV2 makes a nice general counterstain when looking at neuroanatomy in transgenic larvae. We also use this antibody extensively to look for any defects in brain development in mutant larvae. 

Mouse monoclonal anti-acetylated tubulin (IgG2b) (alpha-tubulin, Sigma, Cat# T7451, dilution 1:250)

Select images

woodshole_tubulin_labelled.gif
woodshole_tubulin2_labelled.gif

External Links:

 ZFIN:https://zfin.org/ZDB-ATB-081003-6


LABELS THESE BRAIN STRUCTURES:

Axonal tracts. 


KEY PUBLICATIONS

Wilson, S.W., Ross, L.S., Parrett, T., and Easter, S.S. Jr. (1990)
The development of a simple scaffold of axon tracts in the brain of the embryonic zebrafish, Brachydanio rerio. 
Development (Cambridge, England). 108:121-145

Chitnis, A.B. and Kuwada, J.Y. (1990) 
Axonogenesis in the brain of zebrafish embryos. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 10:1892-1905

Karlstrom, R.O., Trowe, T., Klostermann, S., Baier, H., Brand, M., Crawford, A.D., Grunewald, B., Haffter, P., Hoffman, H., Meyer, S.U., Muller, B.K., Richter, S., van Eeden, F.J., Nüsslein-Volhard, C., and Bonhoeffer, F. (1996) 
Zebrafish mutations affecting retinotectal axon pathfinding. 
Development (Cambridge, England). 123:427-438