Proliferating cell nuclear antigen (PCNA)

Proliferating cell nuclear antigen (PCNA)

ABOUT THIS ANTIBODY

Anti-PCNA labels proliferating cells

Proliferating cell nuclear antigen (PCNA) antibody which binds to the PCNA p36 protein highly expressed in proliferating cells and found to stain proliferating cells in the zebrafish (Mazzitelli-Fuentes et al., 2022). In the killifish it stained many cells in the ganglion cell and outer nuclear layer. Additionally, it labels cell walls in the inner nuclear layer.

Mouse monoclonal anti-PCNA (Santa Cruz, Cat#sc-56, dilution 1:250)

image

by Eva-Maria Breitenbach

Section of 2 mpf female killifish sodium citrate antigen retrieval anti-PCNA (magenta) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

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labels these retinal cell types

Proliferating cells

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key publications

Mazzitelli-Fuentes LS, Román FR, Elías JRC, Deleglise EB and Mongiat LA. 2022. Spatial Learning Promotes Adult Neurogenesis in Specific Regions of the Zebrafish Pallium. Frontiers in Cell and Developmental Biology. 10:1-11.