Glutamine Synthetase (GS)

Glutamine Synthetase (GS)

ABOUT THIS ANTIBODY

Anti-GS labels Müller glia

Glutamine synthetase (GS) protein is expressed in Müller glia. The GS antibody has been found to label Müller glia in zebrafish (Gramage et al., 2015). In the killifish, it labelled Müller glia, however not the whole cell.

Mouse monoclonal anti-GS (Millipore, Cat#MAB302, dilution 1:150)

image

by Eva-Maria Breitenbach

Section of 2 mpf female killifish sodium citrate antigen retrieval anti-GS (magenta) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

labels these retinal cell types

Müller glia

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key publications

Gramage E, D’Cruz T, Taylor S, Thummel R and Hitchcock PF. 2015, Midkine-a protein localization in the developing and adult retina of the zebrafish and its function during photoreceptor regeneration. PLoS One .10:1-17.

Glutamine Synthetase (GS)

Glutamine Synthetase (GS)

ABOUT THIS ANTIBODY

Mouse monoclonal anti-GS (Proteintech, Cat#66323-1-Ig, dilution 1:100)

imageS

by Ryan MacDonald

Section of killifish retina anti-GS

Section of killifish retina anti-GS in magenta and DAPI in blue

Section of killifish retina anti-GS in magenta, anti-Ribeye-A in green and DAPI in blue

labels these retinal cell types

Müller glia

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Retinaldehyde binding protein 1 (Rblp1)

Retinaldehyde binding protein 1 (Rblp1) [CRALBP]

ABOUT THIS ANTIBODY

Also known as CRALBP

Rabbit Polyclonal anti-Rblp1 (Proteintech, Cat#15356-1-AP, dilution 1:100)

imageS

by Ryan MacDonald

Section of killifish retina anti-Rblp1 in green and DAPI in blue

Section of killifish retina anti-Rblp1

Section of killifish retina anti-Rblp1 in green and DAPI in blue

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labels these retinal cell types

Müller glia

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Vimentin

Vimentin

ABOUT THIS ANTIBODY

Mouse monoclonal anti-Vimentin (Proteintech, Cat#60330-1-IG, dilution 1:100)

image

by Ryan MacDonald

Section of young killifish retina anti-Vimentin in magenta and DAPI in blue

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labels these retinal cell types

Müller glia

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Yes associated protein (YAP)

Yes associated protein (YAP)

ABOUT THIS ANTIBODY

Anti-YAP labels Müller glia proliferation

Yes associated protein (YAP) is part of the hippo pathway and its antibody has been found to mark proliferating Müller glia in rats (Zhang and Kong, 2020). In the killifish it stained some cells in the ganglion cell layer, possibly Müller glia cells.

Mouse Monoclonal anti-YAP (Santa Cruz, Cat#sc-101199, dilution 1:200)

imageS

Section of killifish retina anti-YAP

Section of killifish retina anti-YAP in magenta and DAPI in blue

Image by Ryan MacDonald

Section of the ciliary marginal zone of the killifish retina anti-YAP in magenta and DAPI in blue

Image by Ryan MacDonald

Section of 4 mpf female killifish sodium citrate antigen retrieval anti-YAP (magenta) and DAPI (blue)

Image by Eva-Maria Breitenbach

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

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labels these retinal cell types

Müller glia proliferation

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key publications

Zhang W and Kong Y. 2020. YAP is essential for TGF‐β‐induced retinal fibrosis in diabetic rats via promoting the fibrogenic activity of Müller cells. Journal of Cellular and Molecular Medicine. 24:12390-12400.