Yes associated protein (YAP)

ABOUT THIS ANTIBODY

Anti-YAP labels Müller glia proliferation

Yes associated protein (YAP) is part of the hippo pathway and its antibody has been found to mark proliferating Müller glia in rats (Zhang and Kong, 2020). In the killifish it stained some cells in the ganglion cell layer, possibly Müller glia cells.

Mouse Monoclonal anti-YAP (Santa Cruz, Cat#sc-101199, dilution 1:200)

imageS

Section of killifish retina anti-YAP

Section of killifish retina anti-YAP in magenta and DAPI in blue

Image by Ryan MacDonald

Section of the ciliary marginal zone of the killifish retina anti-YAP in magenta and DAPI in blue

Image by Ryan MacDonald

Section of 4 mpf female killifish sodium citrate antigen retrieval anti-YAP (magenta) and DAPI (blue)

Image by Eva-Maria Breitenbach

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

labels these retinal cell types

Müller glia proliferation

key publications

Zhang W and Kong Y. 2020. YAP is essential for TGF‐β‐induced retinal fibrosis in diabetic rats via promoting the fibrogenic activity of Müller cells. Journal of Cellular and Molecular Medicine. 24:12390-12400.