Viewing entries tagged
RGC

Tg(atoh7 :gapRFP)cu2Tg

Tg(atoh7 :gapRFP)cu2Tg

About

 atonal bHLH transcription factor 7 is turned on in retinal ganglion cells as they transition from proliferating neuroblast to differentiated neuron. The atoh7 promoter in this transgenic line drives the expression of a membrane tagged version of RFP so the axons of the RGCs are also labelled so the optic nerve and tract projecting from the retina to the optic tectum can be distinguished.


External Links:

 ZFIN

Lab or Origin: Bill Harris Lab


Expressed in: 

Retinal ganglion cells, cilliary marginal zone, retina, optic nerve. optic tract, optic tectum.  


Key Publications

 Zolessi, F.R., Poggi, L., Wilkinson, C.J., Chien, C.B., and Harris, W.A. (2006)
Polarization and orientation of retinal ganglion cells in vivo.
Neural Development. 1:2.

 Tg(pou4f1-hsp70l:GFP)

Tg(pou4f1-hsp70l:GFP)

About

Tg(pou4f1-hsp70l:GFP) formally Tg(brn3a-hsp70:GFP) expresses green fluorescent protein (GFP) under the control of enhancer elements of brn3a, a POU-domain transcription-factor-encoding gene expressed in the habenula, retina, optic tectum, torus semicircularis and cranial sensory ganglia including the neuromasts of the lateral line and inner ear.

GFP is expressed in retinal ganglion cells and amacrine cells. Projections from RGCs can be seen traversing the optic nerve and innervating the superficial neuropil layer of the optic tectum. In addition, there were neurons expressing GFP in the deeper layers of the tectum. Given that the tectum sends motor outputs from the deeper layers it is likely that these neurons were involved in sending motor outputs to the hindbrain.

The tectobulbar tract descends from the deeper layers of the optic tectum ipsilaterally to connect with the reticulospinal neurons in the hindbrain (Sato et al., 2007).

This transgenic expresses GFP in the dorsal medial habenula subnuclei. Projections from dHbM neurons to the IPN can be seen traversing the fasiculus retroflexus and terminating in the Interpeduncular nuclei. Habenula axons exhibit a spiralling terminal morphology when innervating the IPN (Aizawa et al., 2005).

 


External Links:

 ZFIN

Lab of Origin: Hitoshi Okamoto Lab



Transgene expressed in: 

dorsal habenula, retina, optic tectum, tecto-bulbar tract, fasiculus retroflexus, neuromasts of lateral line, cranial nerve ganglia, hair cells of inner ear.

Key Publications

Sato, T., Hamaoka, T., Aizawa, H., Hosoya, T., and Okamoto, H. (2007)
Genetic single-cell mosaic analysis implicates ephrinB2 reverse signaling in projections from the posterior tectum to the hindbrain in zebrafish.
The Journal of Neuroscience 27(20):5271-5279.

Aizawa, H., Bianco, I.H., Hamaoka, T., Miyashita, T., Uemura, O., Concha, M.L., Russell, C., Wilson, S.W., and Okamoto, H. (2005)
Laterotopic Representation of Left-Right Information onto the Dorso-Ventral Axis of a Zebrafish Midbrain Target Nucleus.
Current biology. 15(3):238-243.

deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E. (2014) Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish.
Genesis (New York, N.Y. : 2000). 52(6):636-55.

 

Tg(-2.7shh:GFP)

Tg(-2.7shh:GFP)

About

The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish  two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.


All images of shh:GFP were produced by Monica Folgueira.

External Links:

 ZFIN

Lab or Origin: Nüsslein-Volhard, Christiane


Expressed in: 

basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.

 


Key Publications

 Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.

Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176