Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.

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Et(fos:Gal4-VP16)s1026t 4dpf dorsal

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker TOTO-3(grey).

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

Single z-slice through the eye.

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf ventral

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

single z-slice at level of subpallium

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at level of preglomerular complex and hypothlamus.

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice through the posterior tuberculum

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the prethalmus and thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the ventral habenulae

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the dorsal habenulae and pineal organ.

External Links:

 ZFIN

Lab or Origin:

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Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.

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Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Et(fos:Gal4-VP16)s1137t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It shows dense expression of kaede in the telencephalon and throughout all the habenula subnuclei. The insertion of this transgene is currently unmapped.

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Et(fos:Gal4-VP16)s1137t lateral 4dpf with SV2 and tubulin

Showing broad telencephalic expression. Also expresses GFP in the habenula, posterior tuberculum and hypothalamus.

Et(fos:Gal4-VP16)s1137t lateral 4dpf with SV2

Showing broad telencephalic expression. Also expresses GFP in the habenula, posterior tuberculum and hypothalamus.

Et(fos:Gal4-VP16)s1137t lateral 4dpf

ventral habenula expression inEt(fos:Gal4-VP16)s1137t

dHbm expression in Et(fos:Gal4-VP16)s1137t

dHbl expression in Et(fos:Gal4-VP16)s1137t

External Links:

 ZFIN

Lab or Origin: Baier Laboratory

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Expressed in: 

 telencephalon, posterior tuberculum, habenula, hypothalamus

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Key Publications

Mason, L., Scott, E.K., Staub, W., Finger-Baier, K., and Baier, H. (2009) Expression patterns from GAL4 enhancer trap screen. ZFIN Direct Data Submission. . (http://zfin.org).

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13. 

Tg(slc17a6b: DsRed)nns9Tg

About

 Slc17a6b is a vesicular glutamate transporter that that mediates the uptake of the excitatory neurotransmitter glutamate into vesicles in the presynaptic terminals of excitatory neurons. This BAC transgenic line from the Yoshihara lab drives the expression of DSRed in glutamatergic neurons. Expression can be seen in many neurons throughout the brain. Strong expression in the dorsal and ventral habenular subnuclei, olfactory bulbs, the pallium and optic tectum

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Tg(slc17a6b:DsRed), tubulin and SV2 24hpf frontal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin and SV2 hpf dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin and SV2 48hpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 48hpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green

Tg(slc17a6b:DsRed) 48hpf Lateral

Tg(slc17a6b:DsRed), tubulin, SV2 48hpf Dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed) 48hpf Dorsal

Tg(slc17a6b:DsRed), tubulin 4dpf Dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green;

Tg(slc17a6b:DsRed) 4dpf Dorsal

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed) 4dpf Lateral

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed) and tubulin 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed) 4dpf Lateral

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Ventral

g(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 4dpf Ventral

g(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Ventral

External Links:

 ZFIN

Lab or Origin: Yoshihara Lab

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Expressed in: 

 glutamatergic neurons, olfactory epithelium, olfactory bulb, pallium, subpallium, dorsal habenula, ventral habenula, preoptic area, prethalamus, pretectum (AF9), optic tectum, trigeminal sensory ganglion.

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Key Publications

 
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009)
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish.
The Journal of neuroscience. 29(15):4756-4767.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum.
Developmental Biology. 343(1-2):1-17.

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

About

The construct used to create this enhancer trap line contains the highly conserved noncoding element  "hs7" from the regulatory region of the human SOX3 gene.

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sox3h7:EGFP with SV2 lateral view 4dpf

sox3h7:EGFP lateral view 4dpf

External Links:

 ZFIN

Lab or Origin: Tom Becker Lab

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Expressed in: 

 olfactory bulb, subpallium, habenula, AF9 pretectum, optic tectum, cerebellum.

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Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.

sox3h10:EGFP

About

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sox3h10:EGFP 5dpf lateral

pallial neuron in sox3h10 lateral view at 5dpf

Cilliary marginal zone expression in sox3h10:EGFP

lateral view of retina at 5dpf

Cilliary marginal zone expression in sox3h10:EGFP

lateral view of retina at 5dpf

sox3h10:EGFP and tubulin dorsal view at 5dpf

showing expreession in pineal and habenulae.

sox3h10:EGFP and tubulin dorsal view of hindbrain at 5dpf

sox3h10:EGFP dorsal view of hindbrain at 5dpf

sox3h10:EGFP dorsal diencephalon at 5dpf

sox3h10:EGFP and tubulin Lateral view of hindbrain

5dpf

sox3h10:EGFP and tubulin Lateral view

5dpf

sox3h10:EGFP and tubulin Lateral view 2dpf

sox3h10:EGFP Lateral view at 2dpf

sox3h10:EGFP and tubulin dorsal view 2dpf

sox3h10:EGFP dorsal view 2dpf

External Links:

 ZFIN

Lab or Origin: Tom Becker Lab

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Expressed in: 

 ciliary marginal zone of retina,pallium, pineal, habenula, hindbrain

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Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.