Viewing entries tagged
medulla oblongata

Et(krt4:EGFP)sqet24

Et(krt4:EGFP)sqet24

About

 Enhancer trap line from Vladimir Korzh lab that has EGFP expression in the epithalamus, thalamic eminence, hypothalamic lobes, , cerebellum, medulla oblongata.


External Links:

 ZFIN

Lab or Origin: Korzh Lab


Expressed in: 

epithalamus, thalamic eminence, hypothalamus, cerebellum, medulla oblongata


Key Publications

Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004)
Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo.
Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

pku2Et/ ETvmat2:GFP

pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.


External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group


Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.


Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.