pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.

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4dpf Ventral view of ETvmat2:GFP.

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Ventral view of ETvmat2:GFP with acetylated tubulin(magenta)..

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Lateral view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf Lateral view of ETvmat2:GFP

4dpf dorsal view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf dorsal view of ETvmat2:GFP.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf Ventral view of ETvmat2:GFP

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf dorsal view of ETvmat2:GFP forbrain with acetylated tubulin(cyan) and SV2(red).

5dpf dorsal view of ETvmat2:GFP forebrain.

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455431398154TimeStamp=14:57:11.398 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=6faacf28-fbff-43f2-905a-d9bbdeb24be2

dpf dorsal view of ETvmat2:GFP hindbrain with acetylated tubulin(cyan) and SV2(red).

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455437197463TimeStamp=14:57:17.197 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=201a84ae-28f4-4eba-9aea-281b8840f135

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the olfactory bulb and telencephalon.

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

the telencephalon has been removed to show monoaminergic cells in the torus loingitudinalis and pretectum.

5dpf dorsal view of ETvmat2:GFP hindbrain.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

5dpf Ventral view of ETvmat2:GFP forebrain with acetylated tubulin(cyan).

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Ventral view of ETvmat2:GFP forebrain.

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Lateral view of ETvmat2:GFP diencephalon with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

5dpf Lateral view of ETvmat2:GFP diencephalon

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group

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Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.

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Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.