This transgenic line was made in the Wilson Lab by Kate Turner, using the pet1 promoter cloned by Lillesaar et al., 2009. The promoter drives KalTA4 and was cloned into a tol2 backbone using the Gateway cloning method (Kwan et al., 2007).

Tg(Pet1:KalTA4) drives expression in serotinergic raphe neurons. In the images below, this driver line was crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2(Auer et al., 2014) and larvae were labelled with anti-RFP and anti-SV2 antibodies to visualise serotinergic raphe neurons and their projections. For a detailed description of the serotinergic system in zebrafish read the excellent Lillesaar et al., 2009 paper. For an overview of the serotinergic system look at our neurotransmitters section.

External Links:


Lab or Origin: Wilson Lab

Expressed in: 

superior raphe, inferior raphe,  serotinergic neurons

Key Publications

Lillesaar, C., Stigloher, C., Tannhäuser, B., Wullimann, M.F., and Bally-Cuif, L. (2009)
Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish, Danio rerio, using transgenics to visualize raphe-specific pet1 expression.
The Journal of comparative neurology. 512(2):158-182.