This transgenic line was made in the Wilson Lab by Kate Turner, using the pet1 promoter cloned by Lillesaar et al., 2009. The promoter drives KalTA4 and was cloned into a tol2 backbone using the Gateway cloning method (Kwan et al., 2007).
Tg(Pet1:KalTA4) drives expression in serotinergic raphe neurons. In the images below, this driver line was crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2(Auer et al., 2014) and larvae were labelled with anti-RFP and anti-SV2 antibodies to visualise serotinergic raphe neurons and their projections. For a detailed description of the serotinergic system in zebrafish read the excellent Lillesaar et al., 2009 paper. For an overview of the serotinergic system look at our neurotransmitters section.
Lab or Origin: Wilson Lab
superior raphe, inferior raphe, serotinergic neurons
Lillesaar, C., Stigloher, C., Tannhäuser, B., Wullimann, M.F., and Bally-Cuif, L. (2009)
Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish, Danio rerio, using transgenics to visualize raphe-specific pet1 expression.
The Journal of comparative neurology. 512(2):158-182.