Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.

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TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP and tubulin lateral view at 4dpf

hindbrain

TG44:EGFP lateral view at 4dpf

hindbrain

TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP dorsal view at 4dpf

hindbrain

TG44:EGFP dorsal view at 4dpf

section through hindbrain showing midline cells

TG44:EGFP and tubulin dorsal view at 4dpf

section through hindbrain showing lateral cells surrounding axon tract.

External Links:

 ZFIN

Lab or Origin: Skarmeta Lab

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Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum

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Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Tg(atoh7 :gapRFP)cu2Tg

About

 atonal bHLH transcription factor 7 is turned on in retinal ganglion cells as they transition from proliferating neuroblast to differentiated neuron. The atoh7 promoter in this transgenic line drives the expression of a membrane tagged version of RFP so the axons of the RGCs are also labelled so the optic nerve and tract projecting from the retina to the optic tectum can be distinguished.

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External Links:

 ZFIN

Lab or Origin: Bill Harris Lab

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Expressed in: 

Retinal ganglion cells, cilliary marginal zone, retina, optic nerve. optic tract, optic tectum.  

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Key Publications

 Zolessi, F.R., Poggi, L., Wilkinson, C.J., Chien, C.B., and Harris, W.A. (2006)
Polarization and orientation of retinal ganglion cells in vivo.
Neural Development. 1:2.

Tg(-8.0cldnb:lynGFP)zf106

About

construct:
Tg(-8.0cldnb:LY-EGFP)

This transgenic line was created by the Gilmore lab “ Eight kilobases of sequence directly upstream of the Claudin B start codon were amplified from BAC zK241F11 by using the Expand Long Template PCR System (Roche). The resultant fragment was cloned into a vector containing lynEGFPpA (Koster and Fraser, 2001) flanked by sites for I-SceI, and the resultant construct was injected into one-cell zebrafish embryos by following the meganuclease transgenesis protocol (Thermes et al., 2002).”(Haas & Gilmore, 2006).

The Gilmore lab wanted to label the lateral line and neuromasts of the lateral line system, one allele of this transgenic also had EGFP expression in the nasal retina and telencephalon and has been used by other labs to study eye and telencephalic morphogenesis.

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Tg(-8.0cldnb:lynGFP)zf106 5dpf lateral

Tg(-8.0cldnb:lynGFP)zf106 5dpf lateral with acetylated tubulin(cyan).

Tg(-8.0cldnb:lynGFP)zf106 5dpf lateral with acetylated tubulin(red).

Tg(-8.0cldnb:lynGFP)zf106 5dpf dorsal with acetylated tubulin(red).

hindbrain

Tg(-8.0cldnb:lynGFP)zf106 2dpf frontal transverse section through the telencephalon

Tg(-8.0cldnb:lynGFP)zf106 2dpf frontal transverse section through the telencephalon

Tg(-8.0cldnb:lynGFP)zf106 2dpf frontal transverse section through the telencephalon

Tg(-8.0cldnb:lynGFP)zf106 2dpf dorsal

Tg(-8.0cldnb:lynGFP)zf106 24hpf dorsal horizontal section through the telencephalon

Tg(-8.0cldnb:lynGFP)zf106 24hpf dorsal horizontal section through the telencephalon and retina

Tg(-8.0cldnb:lynGFP)zf106 24hpf dorsal.

Tg(-8.0cldnb:lynGFP)zf106 24hpf lateral with zo1(red) and acetylated tubulin(cyan).

Tg(-8.0cldnb:lynGFP)zf106 24hpf lateral with and acetylated tubulin(cyan).

Tg(-8.0cldnb:lynGFP)zf106 24hpf lateral

Tg(-8.0cldnb:lynGFP)zf106 adult dorsal with zo1(red)

telencephalon

Tg(-8.0cldnb:lynGFP)zf106 dorsal 4dpf

This image shows the GFP+ aferents innervating the left dorsal habenula.

Tg(-8.0cldnb:lynGFP)zf106 lateral 4dpf Left side

Abbreviations; lfb, lateral forebrain bundle ,OB, olfactory bulb; Pal, pallium; OT, optic tectum; PTh, prethalamus; tHc, tract of the habenula commissure; V, subpallium.

Tg(-8.0cldnb:lynGFP)zf106 lateral 4dpf right side

Abbreviations; lfb, lateral forebrain bundle ,OB, olfactory bulb; Pal, pallium; OT, optic tectum; PTh, prethalamus; tHc, tract of the habenula commissure; V, subpallium.

Tg(-8.0cldnb:lynGFP)zf106 dorsal 48hpf with acetylated tubulin(cyan)

tHC, tract of the habenula commissure.

Tg(-8.0cldnb:lynGFP)zf106 dorsal 48hpf

tHC, tract of the habenula commissure.

Tg(-8.0cldnb:lynGFP)zf106 lateral 48hpf with acetylated tubulin(cyan) and SV2(magenta)

Tg(-8.0cldnb:lynGFP)zf106 lateral 48hpf with SV2(magenta)

External Links:

 ZFIN

Lab or Origin: Gilmore Lab

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Expressed in: 

neuromasts, lateral line, olfactory epithelium, olfactory bulb, , pallium, subpallium, tract of the habenula commissure, nasal retina.

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Key Publications

 Haas, P., and Gilmour, D. (2006)
Chemokine signaling mediates self-organizing tissue migration in the zebrafish lateral line.
Developmental Cell. 10(5):673-680.

Folgueira, M., Bayley, P., Navratilova, P., Becker, T.S., Wilson, S.W., and Clarke, J.D. (2012)
Morphogenesis underlying the development of the everted teleost telencephalon.
Neural Development. 7(1):32.

Valdivia, L.E., Young, R.M., Hawkins, T.A., Stickney, H.L., Cavodeassi, F., Schwarz, Q., Pullin, L.M., Villegas, R., Moro, E., Argenton, F., Allende, M.L., and Wilson, S.W. (2011)
Lef1-dependent Wnt/β-catenin signalling drives the proliferative engine that maintains tissue homeostasis during lateral line development.
Development (Cambridge, England). 138(18):3931-3941.

Valentin, G., Haas, P., and Gilmour, D. (2007)
The chemokine SDF1a coordinates tissue migration through the spatially restricted activation of Cxcr7 and Cxcr4b.
Current biology : CB. 17(12):1026-1031.

Picker, A., Cavodeassi, F., Machate, A., Bernauer, S., Hans, S., Abe, G., Kawakami, K., Wilson, S.W., and Brand, M. (2009)
Dynamic coupling of pattern formation and morphogenesis in the developing vertebrate retina.
PLoS Biology. 7(10):e1000214.

Et(CLG-YFP)smb750

About

 synonyms: clgy750, smb750Et.

This enhancer trap line was generated as part of a large enhancer screen performed by the Becker Lab. This enhancer trap transgenic line shows expression of YFP in the  olfactory bulb, telencephalon, pretectum, torus longitudinalis, cerebellum.

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Et(CLG-YFP)smb750Et 4dpf lateral.

Et(CLG-YFP)smb750Et 4dpf lateral with acetylated tubulin(cyan).

Et(CLG-YFP)smb750Et 4dpf dorsal

Et(CLG-YFP)smb750Et 4dpf dorsal with acetylated tubulin(cyan).

Et(CLG-YFP)smb750Et 4dpf dorsal.

Et(CLG-YFP)smb750Et 4dpf dorsal with acetylated tubulin(cyan).

External Links:

 ZFIN

Lab or Origin: Becker Lab

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Expressed in: 

 olfactory bulb, telencephalon, pretectum, torus longitudinalis, cerebellum.

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Key Publications

 Kikuta, H., Laplante, M., Navratilova, P., Komisarczuk, A.Z., Engstrom, P.G., Fredman, D., Akalin, A., Caccamo, M., Sealy, I., Howe, K., Ghislain, J., Pezeron, G., Mourrain, P., Ellingsen, S., Oates, A.C., Thisse, C., Thisse, B., Foucher, I., Adolf, B., Geling, A., Lenhard, B., and Becker, T.S. (2007)
Genomic regulatory blocks encompass multiple neighboring genes and maintain conserved synteny in vertebrates.
Genome research. 17(5):545-555.

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

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Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 

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More images

Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green), SV2(cyan) and tubulin(red) antibodies.

by Monica Folgueira

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP and anti-tubulin(cyan).

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP.

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green).

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and anti-tubulin antibodies.

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and tubulin(cyan) antibodies.

by Kate Turner

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

by KateTurner

Olfactory glomeruli in Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

Frontal view showing the GFP +processes of olfactory bulb interneurons innervating the glomeruli.

Monica Folgueira

24hpf dorsal view of dlx:GFP with tubulin

Tubulin shows the longitudinal axon scaffold formed by this early stage. Several clusters of dlx:GFP + cells are present in the telencephalon, diencephalon, midbrain and hindbrain.

Monica Folgueira

Olfactory bulb Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

single section through a 4dpf olfactory bulb.

Monica Folgueira

3dpf frontal view of dlx:GFP larvae

Monica Folgueira

3dpf dorsal view dlx:GFP

Showing many GFP+ neurons in the optic tectum and cerebellum.

Monica Folgueira

2dpf dlx:GFP ventral view

Shows cells throughout the subpallium and preoptic area and hypothalamic lobes.

Monica Folgueira

3dpf lateral view of dlx:GFP in forebrain

Monica Folgueira

3dpf late view of dlx:GFP hindbrain

showing dense cluster of GFP+ cells in cerebellum and scattered cells in the spinal cord.

Monica Folgueira

3dpf dorsal view of dlx:GFP hindbrain

Monica Folgueira

3dpf frontal section of dlx:GFP subpallium

Monica Folgueira

3dpf thick section of dlx:GFP through midbrain/diencephalon

Shows tectal and pretectal expression.

Monica Folgueira

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab

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Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

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Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Et(fos:Gal4-VP16)s1168t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. Driving Kaede expression in the posterior tuberculum,hypothalamus and cerebellum. There is a very interesting tract labelled that projects between the midbrain tegmentum and posterior tuberculum. The tract skirts the tectal neuropil. The insertion of this transgene is currently unmapped.

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Lateral views of 4dpf s1168t larvae

kaede (green), SV2(magenta), tubulin(cyan)

Ventral views of 4dpf s1168t larvae

kaede(green); SV2(magenta:tubulin(cyan)

Dorsal views of s1168t larvae

kaede (green), SV2(magenta), tubulin(cyan)

External Links:

 ZFIN

Lab or Origin: Baier Lab

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Expressed in: 

tectum, posterior tuberculum, tegmentum, hypothalamus, cerebellum. 

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Key Publications

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Heap, L.A., Goh, C.C., Kassahn, K.S., and Scott, E.K. (2013) Cerebellar output in zebrafish: an analysis of spatial patterns and topography in eurydendroid cell projections. Frontiers in neural circuits. 7:53.

Heap, L.A., Vanwalleghem, G.C., Thompson, A.W., Favre-Bulle, I., Rubinsztein-Dunlop, H., Scott, E.K. (2018) Hypothalamic Projections to the Optic Tectum in Larval Zebrafish. Frontiers in Neuroanatomy. 11:135.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum. Developmental Biology. 343(1-2):1-17. 

Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.

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Et(fos:Gal4-VP16)s1026t 4dpf dorsal

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker TOTO-3(grey).

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

Single z-slice through the eye.

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf ventral

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

single z-slice at level of subpallium

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at level of preglomerular complex and hypothlamus.

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice through the posterior tuberculum

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the prethalmus and thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the ventral habenulae

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the dorsal habenulae and pineal organ.

External Links:

 ZFIN

Lab or Origin:

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Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.

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Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Et(fos:Gal4-VP16)s1137t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It shows dense expression of kaede in the telencephalon and throughout all the habenula subnuclei. The insertion of this transgene is currently unmapped.

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Et(fos:Gal4-VP16)s1137t lateral 4dpf with SV2 and tubulin

Showing broad telencephalic expression. Also expresses GFP in the habenula, posterior tuberculum and hypothalamus.

Et(fos:Gal4-VP16)s1137t lateral 4dpf with SV2

Showing broad telencephalic expression. Also expresses GFP in the habenula, posterior tuberculum and hypothalamus.

Et(fos:Gal4-VP16)s1137t lateral 4dpf

ventral habenula expression inEt(fos:Gal4-VP16)s1137t

dHbm expression in Et(fos:Gal4-VP16)s1137t

dHbl expression in Et(fos:Gal4-VP16)s1137t

External Links:

 ZFIN

Lab or Origin: Baier Laboratory

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Expressed in: 

 telencephalon, posterior tuberculum, habenula, hypothalamus

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Key Publications

Mason, L., Scott, E.K., Staub, W., Finger-Baier, K., and Baier, H. (2009) Expression patterns from GAL4 enhancer trap screen. ZFIN Direct Data Submission. . (http://zfin.org).

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13. 

Tg(gata2:eGFP)bi105

About

The Tg(gata2:eGFP)bi105 enhancer trap transgenic line was generated as part of an enhancer trap screen by the Becker Lab using the Tol2-transposase system. It has GFP expression throughout the pallium, in a small subpallial nucleus, parapineal organ, optic tectum, pretectum, posterior tuberculum and lateral hypothalamus. In the hindbrain, several cell bodies are labelled in the superior raphe and areas of the reticular formation and caudally in areas of the medulla oblongata

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External Links:

 ZFIN

Lab of Origin: Tom Becker Lab

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Transgene expressed in: 

Key Publications

Folgueira, M., Bayley, P., Navratilova, P., Becker, T.S., Wilson, S.W., and Clarke, J.D. (2012)
Morphogenesis underlying the development of the everted teleost telencephalon.
Neural Development. 7(1):32

Ragvin, A., Moro, E., Fredman, D., Navratilova, P., Drivenes, O., Engström, P.G., Alonso, M.E., Mustienes, E.D., Gomez Skarmeta, J.L., Tavares, M.J., Casares, F., Manzanares, M., van Heyningen, V., Molven, A., Njølstad, P.R., Argenton, F., Lenhard, B., and Becker, T.S. (2010)
Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3.
Proceedings of the National Academy of Sciences of the United States of America. 107(2):775-780

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30

Tg(glyt2:GFP)/Tg(slc6a5:GFP)

About

 Synonyms: Tg(slc6a5:GFP), Tg(slc6a5:GFP)cf3, cf3Tg

Previously called glycine transporter 2 now named solute carrier family 6 member 5(slc6a5), this trangenic line labels glycinergic neruons.

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Tg(slc6a5:GFP) dorsal 4dpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 4dpf

Tg(slc6a5:GFP) dorsal 4dpf with tubulin(cyan)

Pineal organ

Tg(slc6a5:GFP) dorsal 4dpf

Pineal organ

Tg(slc6a5:GFP) dorsal 4dpf hindbrain with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 4dpf hindbrain

Tg(slc6a5:GFP) lateral 4dpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 4dpf

Tg(slc6a5:GFP) ventral 4dpf with tubulin(cyan)

Single z-plane

Tg(slc6a5:GFP) ventral 4dpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 48hpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 48hpf

Tg(slc6a5:GFP) lateral 48hpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 48hpf

Tg(slc6a5:GFP) dorsal 48hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 48hpf

Tg(slc6a5:GFP) lateral 48hpf with tubulin(cyan)

spinal cord

Tg(slc6a5:GFP) lateral 48hpf

spinal cord

Tg(slc6a5:GFP) Lateral 24hpf

Tg(slc6a5:GFP) dorsal 30hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 30hpf

Tg(slc6a5:GFP) lateral 30hpf with tubulin(cyan)

hindbrain/anterior spinal cord

Tg(slc6a5:GFP) lateral 30hpf with tubulin(cyan)

spinal cord

Tg(slc6a5:GFP) lateral 30hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 30hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 30hpf

External Links:

 ZFIN

Lab or Origin: Joe Fetcho Lab

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Expressed in: 

 glycinergic neurons, spinal cord, pineal, hindbrain.

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Key Publications

McLean, D.L., Fan, J., Higashijima, S., Hale, M.E., and Fetcho, J.R. (2007)
A topographic map of recruitment in spinal cord.
Nature. 446(7131):71-75.

Barreiro-Iglesias, A., Mysiak, K.S., Adrio, F., Rodicio, M.C., Becker, C.G., Becker, T., and Anadón, R. (2013)
Distribution of glycinergic neurons in the brain of glycine transporter-2 Tg(glyt2:gfp) transgenic adult zebrafish: Relation with brain-spinal descending systems.
The Journal of comparative neurology. 521(2):389-425.
 

Tg(isl1:GFP)rw0

About

 This transgenic line expresses GFP in cranial motor neurons. It was made using a construct that fuses Islet-1 promotor/enhancer sequences to GFP.

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More images of this transgenic

isl1:GFP lateral 1dpf with ZO1(grey)

isl1:GFP lateral 1dpf

isl1:GFP lateral 1dpf GFP and tubulin(cyan)

isl1:GFP lateral 1dpf

isl1:GFP lateral 1dpf with tubulin (cyan)

isl1 lateral 1dpf spinal cord

isl1:GFP ventral 36hpf

isl1 dorsal 1dpf

isl1:GFP 36hpf lateral with tubulin(cyan)

isl1:GFP 36hpf lateral

isl1:GFP 60hpf dorsal with tubulin (cyan)

isl1:GFP 60hpf dorsal

isl1:GFP 60hpf lateral with tubulin (cyan)

isl1:GFP 60hpf lateral

isl1:GFP 60hpf ventral with tubulin (cyan

isl1:GFP 60hpf ventral

isl1:GFP 3dpf dorsal with tubulin(cyan)

isl1:GFP 3dpf dorsal

isl1 lateral 3dpf

isl1:GFP 3dpf lateral forebrain

isl1:GFP 3dpf lateral forebrain with tubulin(cyan)

isl1:GFP dorsal 4dpf with tubulin(cyan)

isl1:GFP dorsal 4dpf

isl1:GFP lateral 4dpf with tubulin(cyan)

isl1:GFP 4dpf lateral

isl1:GFP lateral 5dpf

isl1:GFP 7dpf lateral forebrain with ZO1(grey)

isl1:GFP 7dpf lateral forebrain

External Links:

 ZFIN

Lab or Origin: Okomoto Lab

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Expressed in: 

cranial motor neurons, hindbrain, subpallium, preoptic area. 

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Key Publications

Higashijima, S., Hotta, Y., and Okamoto, H. (2000)
Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer.
The Journal of neuroscience. 20(1):206-218

Suli, A., Mortimer, N., Shepherd, I., and Chien, C.B. (2006)
Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons.
The Journal of neuroscience. 26(51):13328-13337.

Schoppik, D., Bianco, I.H., Prober, D.A., Douglass, A.D., Robson, D.N., Li, J.M.B., Greenwood, J.S.F., Soucy, E., Engert, F., Schier, A.F. (2017)
Gaze-stabilizing central vestibular neurons project asymmetrically to extraocular motoneuron pools. The Journal of neuroscience. 37(47):11353-11365.

Rebman, J.K., Kirchoff, K.E., Walsh, G.S. (2016)
Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons.
PLoS One. 11:e0164433.

Barsh, G.R., Isabella, A.J., Moens, C.B. (2017)
Vagus Motor Neuron Topographic Map Determined by Parallel Mechanisms of hox5 Expression and Time of Axon Initiation.
Current biology : CB. 27(24):3812-3825.e3.

Cox, J.A., Lamora, A., Johnson, S.L., and Voigt, M.M. (2011)
Diverse mechanisms for assembly of branchiomeric nerves.
Developmental Biology. 357(2):305-17.

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.

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VKET11:EGFP and tubulin dorsal view 4dpf

VKET11:EGFP dorsal view 4dpf

VKET11:EGFP and tubulin ventral view 4dpf

VKET11:EGFP ventral view 4dpf

External Links:

 ZFIN

Lab or Origin: Korzh Lab

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Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary

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Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Et(krt4:EGFP)sqet33

About

 Enhancer trap line from Vladimir Korzh lab that has EGFP expression in the roof plate.

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ET33:EGFP and zo1 lateral view at 2dpf

ET33:EGFP and zo1 lateral view at 2dpf

ET33:EGFP frontal view

transverse section at 24hpf showing roof plate expression

ET33:EGFP frontal view

24hpf

ET33:EGFP lateral view 24hpf

ET33:EGFP lateral view 12 somite stage

External Links:

 ZFIN

Lab or Origin: Korzh Lab

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Expressed in: 

 roof plate

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Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Kondrychyn, I., Teh, C., Sin, M., and Korzh, V. (2013) Stretching morphogenesis of the roof plate and formation of the central canal. PLoS One. 8(2):e56219.

Et(krt4:EGFP)sqet24

About

 Enhancer trap line from Vladimir Korzh lab that has EGFP expression in the epithalamus, thalamic eminence, hypothalamic lobes, cerebellum, medulla oblongata.

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Et(krt4:EGFP)sqet24 5dpf ventral

Et(krt4:EGFP)sqet24 5dpf ventral with tubulin (magenta)

Et(krt4:EGFP)sqet24 5dpf lateral

Et(krt4:EGFP)sqet24 5dpf lateral with tubulin (magenta)

Et(krt4:EGFP)sqet24 5dpf lateral

Et(krt4:EGFP)sqet24 5dpf lateral with tubulin (magenta)

External Links:

 ZFIN

Lab or Origin: Korzh Lab

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Expressed in: 

epithalamus, thalamic eminence, hypothalamus, cerebellum, medulla oblongata

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Key Publications

Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004)
Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo.
Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Tg(lhx5:GFP)b1205

About

 This transgenic line recapitulates the expression of lhx5 transcription factor. Generated by the Westerfield lab using BAC transgenesis. A kaede version of this line is also available. This transgenic has been used to study pathfinding in early telencephalic development (Guo et al., 2012; Zhang et al., 2012, Turner et al., 2019) and also to look at areas afferent to the habenula at later stages of development (Turner et al., 2016).

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Tg(lhx5:GFP) 4dpf lateral view

GFP+ populations in the forebrain innervate all habeunula subnuclei.

Abbreviations: Dp; posterior area of the pallium; Hb, habenula; OB, olfactory bulb; PO, preoptic area; PTh, prethalamus; tHC, tract of the habenula commissure; StMed, stria medularis; vENT, ventral entopeduncular nucleus.

Figure taken from Turner et al., 2016.

Tg(lhx5:GFP) lateral view 48hpf.

GFP+ populations in the forebrain innervate all habeunula subnuclei.

Abbreviations: Dp; posterior area of the pallium; Hb, habenula; OB, olfactory bulb; PO, preoptic area; PTh, prethalamus; tHC, tract of the habenula commissure; StMed, stria medularis; vENT, ventral entopeduncular nucleus.

Figure taken from Turner et al., 2016.

Dorsal view of the habenula in a 4dpf Tg(lhx5:GFP) larva.

All habenula subnuclei are innervated by GFP+ processes in the Tg(lhx5:GFP) line.

Abbreviations: dHbl, lateral subnucleus of the dorsal habeula; dHbm, medial subnucleus of the dorsal habenula; vHb, ventral subnucleus of the habenula.

lateral view of an adult Tg(lhx5:GFP) zebrafish

the ventral entopeduncular nucleus(vENT) is strongly labelled in this transgenic line.

Abbreviations: ac, anterior commissure; Pal, pallium; OB, olfactory bulb; vENT, ventral entopeduncular nucleus.

Image from Turner et al., 2016.

transverse section through the telencephalon of a Tg(lhx5:GFP) zebrafish.

the ventral entopeduncular nucleus(vENT) is strongly labelled in this transgenic line.

Abbreviations: ac, anterior commissure; dENT, dorsal entopeduncular nucleus; Dp, posterior area of the pallium; vENT, ventral entopeduncular nucleus.

Image from Turner et al., 2016.

transverse section through the habenula of an adult Tg(lhx5:GFP) zebrafish

All habenula subnuclei are innervated by GFP+ processes in the Tg(lhx5:GFP) line.

Image from Turner et al., 2016

Tg(lhx5:GFP)(cyan) 4dpf dorsal view with SV2(magenta)

Tg(lhx5:GFP)(cyan) 4dpf ventral view with SV2(magenta)

Tg(lhx5:GFP)(cyan) 4dpf lateral view

Tg(lhx5:GFP)(cyan) 4dpf lateral view with SV2(magenta)

External Links:

 ZFIN

Lab or Origin: Westerfield Lab

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Expressed in: 

 olfactory bulb, pallium, ventral entopeduncular nucleus, preoptic area, prethalamus, stria medularis, tract of the habenula commissure, habenula commisure, habenula neuropil.

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Key Publications

Gao, J., Zhang, C., Yang, B., Sun, L., Zhang, C., Westerfield, M., and Peng, G. (2012)
Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish.
PLoS One. 7(5):e36516.

Zhang, C., Gao, J., Zhang, H., Sun, L., and Peng, G. (2012)
Robo2-Slit and Dcc-Netrin1 Coordinate Neuron Axonal Pathfinding within the Embryonic Axon Tracts.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(36):12589-12602.

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30.

Turner, K.J., Hoyle, J., Valdivia, L.E., Cerveny, K.L., Hart, W., Mangoli, M., Geisler, R., Rees, M., Houart, C., Poole, R.J., Wilson, S.W., Gestri, G. (2019)
Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits.
PLoS One. 14:e0211073.

Tg(oxt:EGFP)

About

 This transgenic construct drives GFP expression in oxytocin expressing neurons in the neuro-secretary pre-optic area(NPO) part of the neuroendocrine system.

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Ventral view of 4dpf Tg(oxt:GFP) with tubulin

Ventral view of 4dpf Tg(oxt:GFP)

Ventral view of 4dpf Tg(oxt:GFP)with SV2

ventral view of the NPO at 4dpf

lateral view of the 4dpf Tg(oxt:EGFP)with tubulin and sv2

lateral view of the 4dpf Tg(oxt:EGFP)

External Links:

 ZFIN

Lab or Origin: Gil Levkowitz Lab

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Expressed in: 

preoptic area, neurosecretory preoptic area (NPO).  

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Key Publications

Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G. (2011)
The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary.
Developmental Cell. 21(4):642-654.

Machluf, Y., Gutnick, A., and Levkowitz, G. (2011)
Development of the zebrafish hypothalamus.
Annals of the New York Academy of Sciences. 1220(1):93-105.

Tg(Pet1:KalTA4)

About

 This transgenic line was made in the Wilson Lab by Kate Turner, using the pet1 promoter cloned by Lillesaar et al., 2009. The promoter drives KalTA4 and was cloned into a tol2 backbone using the Gateway cloning method (Kwan et al., 2007).

Tg(Pet1:KalTA4) drives expression in serotinergic raphe neurons. In the images below, this driver line was crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2(Auer et al., 2014) and larvae were labelled with anti-RFP and anti-SV2 antibodies to visualise serotinergic raphe neurons and their projections. For a detailed description of the serotinergic system in zebrafish read the excellent Lillesaar et al., 2009 paper. For an overview of the serotinergic system look at our neurotransmitters section.

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Tg(Pet1:KalTA4) 4dpf lateral view.

crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2 and labelled with anti-RFP(magenta) and anti-SV2(green).

Tg(Pet1:KalTA4) 4dpf lateral view.

crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2 and labelled with anti-RFP(magenta) .

Tg(Pet1:KalTA4) 4dpf dorsal view.

crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2 and labelled with anti-RFP(magenta) and anti-SV2(green).

Tg(Pet1:KalTA4) 4dpf dorsal view.

crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2 and labelled with anti-RFP(magenta) .

Tg(Pet1:KalTA4) 4dpf ventral view.

crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2 and labelled with anti-RFP(magenta) and anti-SV2(green).

Tg(Pet1:KalTA4) 4dpf ventral view.

crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2 and labelled with anti-RFP(magenta) .

External Links:

 ZFIN

Lab or Origin: Wilson Lab

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Expressed in: 

superior raphe, inferior raphe,  serotinergic neurons

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Key Publications

Lillesaar, C., Stigloher, C., Tannhäuser, B., Wullimann, M.F., and Bally-Cuif, L. (2009)
Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish, Danio rerio, using transgenics to visualize raphe-specific pet1 expression.
The Journal of comparative neurology. 512(2):158-182. 

pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.

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4dpf Ventral view of ETvmat2:GFP.

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Ventral view of ETvmat2:GFP with acetylated tubulin(magenta)..

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Lateral view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf Lateral view of ETvmat2:GFP

4dpf dorsal view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf dorsal view of ETvmat2:GFP.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf Ventral view of ETvmat2:GFP

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf dorsal view of ETvmat2:GFP forbrain with acetylated tubulin(cyan) and SV2(red).

5dpf dorsal view of ETvmat2:GFP forebrain.

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455431398154TimeStamp=14:57:11.398 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=6faacf28-fbff-43f2-905a-d9bbdeb24be2

dpf dorsal view of ETvmat2:GFP hindbrain with acetylated tubulin(cyan) and SV2(red).

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455437197463TimeStamp=14:57:17.197 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=201a84ae-28f4-4eba-9aea-281b8840f135

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the olfactory bulb and telencephalon.

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

the telencephalon has been removed to show monoaminergic cells in the torus loingitudinalis and pretectum.

5dpf dorsal view of ETvmat2:GFP hindbrain.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

5dpf Ventral view of ETvmat2:GFP forebrain with acetylated tubulin(cyan).

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Ventral view of ETvmat2:GFP forebrain.

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Lateral view of ETvmat2:GFP diencephalon with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

5dpf Lateral view of ETvmat2:GFP diencephalon

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group

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Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.

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Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.

Tg(pou4f1-hsp70l:GFP)

About

Tg(pou4f1-hsp70l:GFP) formally Tg(brn3a-hsp70:GFP) expresses green fluorescent protein (GFP) under the control of enhancer elements of brn3a, a POU-domain transcription-factor-encoding gene expressed in the habenula, retina, optic tectum, torus semicircularis and cranial sensory ganglia including the neuromasts of the lateral line and inner ear.

GFP is expressed in retinal ganglion cells and amacrine cells. Projections from RGCs can be seen traversing the optic nerve and innervating the superficial neuropil layer of the optic tectum. In addition, there were neurons expressing GFP in the deeper layers of the tectum. Given that the tectum sends motor outputs from the deeper layers it is likely that these neurons were involved in sending motor outputs to the hindbrain.

The tectobulbar tract descends from the deeper layers of the optic tectum ipsilaterally to connect with the reticulospinal neurons in the hindbrain (Sato et al., 2007).

This transgenic expresses GFP in the dorsal medial habenula subnuclei. Projections from dHbM neurons to the IPN can be seen traversing the fasiculus retroflexus and terminating in the Interpeduncular nuclei. Habenula axons exhibit a spiralling terminal morphology when innervating the IPN (Aizawa et al., 2005).

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External Links:

 ZFIN

Lab of Origin: Hitoshi Okamoto Lab

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brn3a_lat_4dpf.png

Lateral view of a 4dpf Tg(pou4f1:hsp70l:GFP) embryo labelled with anti-GFP and anti-tubulin.

brn3a_4dpf_lat_noeye.png

Lateral view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. Eye has been removed to show the optic nerve ascending to the optic tectum.

brn3a_4dpf_ventral.png

view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP. Showing the dicussation of the optic nerve at the optic chiasm.

brn3a_4dpf_vent.png

view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. Showing the dicussation of the optic nerve at the optic chiasm.

brn3a_60hpf_dorsal.png

Dorsal view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. At this stage of development, GFP + dorsal habenula neurons have increased in number. Retinal ganglion cell axons innervate the superficial layer of the optic tectum. GFP positive cells are also present in the deeper tectal layers.

brn3a_60hpf_dorsal_IPN.png

Dorsal view of a 60hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP. This z-projection is cropped to show the habenula afferent terminals spiralling innervation pattern in the IPN.

brn3a_60hpf_lat.png

Lateral view of a 60hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. 

brn3a_36hpf_lateral.png

Lateral view of a 36hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. 

brn3a_48hpf_dorsal.png

Lateral view of a 48hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. 

brn3a_4dpf_cranial ganglia.png

dorsal view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. Showing hair cells of the inner ear and neuromasts of the lateral line.

Brn3a_4dpf_habenula.png

Dorsal view of the habenula in a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP. This transgene is expressed in the medial subnucleus of the dorsal habenula.

brn3a_4dpf_habenula_dapi.png

Dorsal view of the habenula in a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and DAPI. This transgene is expressed in the medial subnucleus of the dorsal habenula.

brn3a_4dpf_habenula_SV2.png

Dorsal view of the habenula in a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-SV2. This transgene is expressed in the medial subnucleus of the dorsal habenula.

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Transgene expressed in: 

dorsal habenula, retina, optic tectum, tecto-bulbar tract, fasiculus retroflexus, neuromasts of lateral line, cranial nerve ganglia, hair cells of inner ear.

Key Publications

Sato, T., Hamaoka, T., Aizawa, H., Hosoya, T., and Okamoto, H. (2007)
Genetic single-cell mosaic analysis implicates ephrinB2 reverse signaling in projections from the posterior tectum to the hindbrain in zebrafish.
The Journal of Neuroscience 27(20):5271-5279.

Aizawa, H., Bianco, I.H., Hamaoka, T., Miyashita, T., Uemura, O., Concha, M.L., Russell, C., Wilson, S.W., and Okamoto, H. (2005)
Laterotopic Representation of Left-Right Information onto the Dorso-Ventral Axis of a Zebrafish Midbrain Target Nucleus.
Current biology. 15(3):238-243.

deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E. (2014) Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish.
Genesis (New York, N.Y. : 2000). 52(6):636-55.

Tg(-2.7shh:GFP)

About

The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish  two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.

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All images of shh:GFP were produced by Monica Folgueira.

4dpf lateral shh:GFP with acetylated tubulin(cyan) and sytox(grey)

pHb, habenula; lfb, lateral forebrain bundle; OB, olfactory bulb; OT, optic tectum; ot, optic tract; Pal, pallium; PT, posterior tuberculum; PTh, prethalamus; Th, thalamus; ZLI, zona-limitans intrathalamica.

shh:GFP, tubulin and sytox 4dpf lateral forebrain

shh:GFP and tubulin. 4 dpf lateral forebrain

shh:GFP and tubulin. 4dpf dorsal view

single z-slice through telencephalon

shh:GFP, tublin and sytox at 4 dpf showing GFP + processes innervating the telencephalon.

shh:GFP dorsal view of 4dpf larvae

Frontal transverse z-slice through the telencephalon of shh:GFP

shh:GFP, tublin and sytox at 4.5dpf showing GFP + processes innervating the telencephalon.

shh:GFP and tubulin, 4dpf lateral view

shh:GFP lateral view 4dpf

shh:GFP, tubulin 4dpf ventral view

Shows expression in floor plate, posterior tuberculum and hypothalamus

shh:GFP and tubulin 24hpf lateral view

shh:GFP lateral forebrain at 24hpf

Shows expression in floor plate and zona limitans intrathalamica.

shh:GFP and tubulin lateral view of 24hpf

External Links:

 ZFIN

Lab or Origin: Nüsslein-Volhard, Christiane

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Expressed in: 

basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.

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Key Publications

 Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.

Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176