Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.

---

TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP and tubulin lateral view at 4dpf

hindbrain

TG44:EGFP lateral view at 4dpf

hindbrain

TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP dorsal view at 4dpf

hindbrain

TG44:EGFP dorsal view at 4dpf

section through hindbrain showing midline cells

TG44:EGFP and tubulin dorsal view at 4dpf

section through hindbrain showing lateral cells surrounding axon tract.

External Links:

 ZFIN

Lab or Origin: Skarmeta Lab

---

Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum

---

Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824