HuC/D

HuC/D

ABOUT THIS ANTIBODY

Anti-HuC/D labels amacrine and ganglion cells in the retina

HuC/D antibody which has been found to label HuC/D neuronal proteins which are expressed in neurons leaving the mitotic cycle. It labels amacrine cells in the inner nuclear layer and ganglion cells in the ganglion cell layer in the zebrafish (Gorsuch et al., 2017). In the killifish it labelled cells in the inner nuclear layer and cells in the ganglion cell layer.

Mouse monoclonal anti-HuC/D (Invitrogen, Cat#A21271, dilution 1:200)

imageS

Section of killifish retina anti-HuC/D in magenta, anti-pkc in green and DAPI in blue

Image by Ryan MacDonald

Section of 2 mpf male killifish sodium citrate antigen retrieval anti-HuC/D in magenta and DAPI in blue

Image by Eva-Maria Breitenbach

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

Section of 2 mpf male killifish sodium citrate antigen retrieval anti-HuC/D (magenta) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

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labels these retinal cell types

Amacrine cells in the inner nuclear layer and ganglion cells in the ganglion cell layer

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key publications

Gorsuch RA, Lahne M, Yarka CE, Petravick ME, Li J and Hyde DR. 2017. Sox2 regulates Müller glia reprogramming and proliferation in the regenerating zebrafish retina via Lin28 and Ascl1a. Experimental Eye Research. 161:174-192.