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Amacrine cells
Rabbit Polyclonal anti-Calbindin (Swant, Cat#1CB38, dilution 1:200)
by Ryan MacDonald
Amacrine and bipolar cells
Anti-HuC/D labels amacrine and ganglion cells in the retina
HuC/D antibody which has been found to label HuC/D neuronal proteins which are expressed in neurons leaving the mitotic cycle. It labels amacrine cells in the inner nuclear layer and ganglion cells in the ganglion cell layer in the zebrafish (Gorsuch et al., 2017). In the killifish it labelled cells in the inner nuclear layer and cells in the ganglion cell layer.
Mouse monoclonal anti-HuC/D (Invitrogen, Cat#A21271, dilution 1:200)
Image by Ryan MacDonald
Image by Eva-Maria Breitenbach
PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer
Section of 2 mpf male killifish sodium citrate antigen retrieval anti-HuC/D (magenta) and DAPI (blue)
PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer
Amacrine cells in the inner nuclear layer and ganglion cells in the ganglion cell layer
Gorsuch RA, Lahne M, Yarka CE, Petravick ME, Li J and Hyde DR. 2017. Sox2 regulates Müller glia reprogramming and proliferation in the regenerating zebrafish retina via Lin28 and Ascl1a. Experimental Eye Research. 161:174-192.
Anti-PV labels amacrine cells
Parvalbumin (PV) antibody binding to parvalbumin which is a regulator of neuronal intracellular calcium dynamics and found to stain amacrine cells in zebrafish (Ahmed, Kojima and Masai, 2022). In the killifish it stained cells in the inner nuclear and ganglion cell layer; and linear speckled staining in the inner nuclear, plexiform and ganglion cell layer.
Mouse Monoclonal anti-PV (Sigma, Cat#P3088, dilution 1:200)
Image by Ryan MacDonald
Image by Ryan MacDonald
Amacrine cells
Ahmed M, Kojima Y and Masai I. 2022. Strip1 regulates retinal ganglion cell survival by suppressing Jun-mediated apoptosis to promote retinal neural circuit formation. eLife. 11:1-34.
Rabbit Polyclonal anti-Pax6 (Proteintech, Cat#12323-1-AP, dilution 1:100)
by Ryan MacDonald
Amacrine cells and ganglion cells
