Proliferating cell nuclear antigen (PCNA)
ABOUT THIS ANTIBODY
Anti-PCNA labels proliferating cells
Proliferating cell nuclear antigen (PCNA) antibody which binds to the PCNA p36 protein highly expressed in proliferating cells and found to stain proliferating cells in the zebrafish (Mazzitelli-Fuentes et al., 2022). In the killifish it stained many cells in the ganglion cell and outer nuclear layer. Additionally, it labels cell walls in the inner nuclear layer.
Mouse monoclonal anti-PCNA (Santa Cruz, Cat#sc-56, dilution 1:250)
image
by Eva-Maria Breitenbach
Section of 2 mpf female killifish sodium citrate antigen retrieval anti-PCNA (magenta) and DAPI (blue)
PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer
labels these retinal cell types
Proliferating cells
key publications
Mazzitelli-Fuentes LS, Román FR, Elías JRC, Deleglise EB and Mongiat LA. 2022. Spatial Learning Promotes Adult Neurogenesis in Specific Regions of the Zebrafish Pallium. Frontiers in Cell and Developmental Biology. 10:1-11.
