Caspase-3

Caspase-3

ABOUT THIS ANTIBODY

Anti-Caspase-3 labels apoptosis

Caspase-3 antibody, caspases, are crucial mediators in apoptosis and caspase-3 is activated during death protease. Its antibody labels apoptosis in mice (Gashegu et al., 2007). In the killifish, we found two cells stained in the ganglion cell layer (white arrow) and some staining in the outer plexiform layer (yellow arrow).

Rabbit Polyclonal anti-Caspase-3 (BD Biosciences, Cat#559565, dilution 1:200)

image

by Eva-Maria Breitenbach

Section of 4 mpf male killifish sodium citrate antigen retrieval anti-Caspase-3 in green and DAPI in blue

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labels these retinal cell types

Cells undergoing apoptosis

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key publications

Gashegu J, Ladha R, Vanmuylder N, Philippson C, Bremer F, Rooze M and Louryan S. 2007. HSP110, caspase‐3 and‐9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all‐trans retinoic acid or irradiation during early mouse eye development. Journal of Anatomy. 210:532-541.

Tumor protein P53

Tumor protein P53

ABOUT THIS ANTIBODY

Anti-P53 labels apoptosis

Tumor protein P53 is a sequence specific transcription factor which is being activated when cells are under stress. P53 antibody stains cell death in mice (Dong et al., 2019). In killifish it labelled one cell in the outer nuclear layer (white arrow).

Rabbit Polyclonal anti-P53 (Proteintech, Cat#21891-1-AP, dilution 1:100)

image

by Eva-Maria Breitenbach

Section of 4 mpf female killifish sodium citrate antigen retrieval anti-P53 (green) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

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labels these retinal cell types

Apoptosis

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key publications

Dong S, Ji J, Hu L and Wang H. 2019. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sciences. 227:20-29.