Viewing entries tagged
hindbrain

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.

External Links:

 ZFIN

Lab or Origin: Skarmeta Lab

Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum

Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Tg(glyt2:GFP)/Tg(slc6a5:GFP)

Tg(glyt2:GFP)/Tg(slc6a5:GFP)

About

 Synonyms: Tg(slc6a5:GFP), Tg(slc6a5:GFP)cf3, cf3Tg

Previously called glycine transporter 2 now named solute carrier family 6 member 5(slc6a5), this trangenic line labels glycinergic neruons.

External Links:

 ZFIN

Lab or Origin: Joe Fetcho Lab

Expressed in: 

 glycinergic neurons, spinal cord, pineal, hindbrain.

Key Publications

McLean, D.L., Fan, J., Higashijima, S., Hale, M.E., and Fetcho, J.R. (2007)
A topographic map of recruitment in spinal cord.
Nature. 446(7131):71-75.

Barreiro-Iglesias, A., Mysiak, K.S., Adrio, F., Rodicio, M.C., Becker, C.G., Becker, T., and Anadón, R. (2013)
Distribution of glycinergic neurons in the brain of glycine transporter-2 Tg(glyt2:gfp) transgenic adult zebrafish: Relation with brain-spinal descending systems.
The Journal of comparative neurology. 521(2):389-425.
 

Tg(isl1:GFP)rw0

Tg(isl1:GFP)rw0

About

 This transgenic line expresses GFP in cranial motor neurons. It was made using a construct that fuses Islet-1 promotor/enhancer sequences to GFP.

isl1 cranial nerves expanded-01.png
isl1 cranial nerves expanded-02.png

More images of this transgenic

External Links:

 ZFIN

Lab or Origin: Okomoto Lab

Expressed in: 

cranial motor neurons, hindbrain, subpallium, preoptic area. 

Key Publications

Higashijima, S., Hotta, Y., and Okamoto, H. (2000)
Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer.
The Journal of neuroscience. 20(1):206-218

Suli, A., Mortimer, N., Shepherd, I., and Chien, C.B. (2006)
Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons.
The Journal of neuroscience. 26(51):13328-13337.

Schoppik, D., Bianco, I.H., Prober, D.A., Douglass, A.D., Robson, D.N., Li, J.M.B., Greenwood, J.S.F., Soucy, E., Engert, F., Schier, A.F. (2017)
Gaze-stabilizing central vestibular neurons project asymmetrically to extraocular motoneuron pools. The Journal of neuroscience. 37(47):11353-11365.

Rebman, J.K., Kirchoff, K.E., Walsh, G.S. (2016)
Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons.
PLoS One. 11:e0164433.

Barsh, G.R., Isabella, A.J., Moens, C.B. (2017)
Vagus Motor Neuron Topographic Map Determined by Parallel Mechanisms of hox5 Expression and Time of Axon Initiation.
Current biology : CB. 27(24):3812-3825.e3.

Cox, J.A., Lamora, A., Johnson, S.L., and Voigt, M.M. (2011)
Diverse mechanisms for assembly of branchiomeric nerves.
Developmental Biology. 357(2):305-17.

sox3h10:EGFP

sox3h10:EGFP

About

 

External Links:

 ZFIN

Lab or Origin: Tom Becker Lab

Expressed in: 

 ciliary marginal zone of retina,pallium, pineal, habenula, hindbrain

Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.