Viewing entries tagged
olfactory bulb

pku2Et/ ETvmat2:GFP

pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.


External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group


Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.


Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.

Tg(-8.0cldnb:lynGFP)zf106

Tg(-8.0cldnb:lynGFP)zf106

About

construct:
Tg(-8.0cldnb:LY-EGFP)

This transgenic line was created by the Gilmore lab “ Eight kilobases of sequence directly upstream of the Claudin B start codon were amplified from BAC zK241F11 by using the Expand Long Template PCR System (Roche). The resultant fragment was cloned into a vector containing lynEGFPpA (Koster and Fraser, 2001) flanked by sites for I-SceI, and the resultant construct was injected into one-cell zebrafish embryos by following the meganuclease transgenesis protocol (Thermes et al., 2002).”(Haas & Gilmore, 2006).

The Gilmore lab wanted to label the lateral line and neuromasts of the lateral line system, one allele of this transgenic also had EGFP expression in the nasal retina and telencephalon and has been used by other labs to study eye and telencephalic morphogenesis.


External Links:

 ZFIN

Lab or Origin: Gilmore Lab


Expressed in: 

neuromasts, lateral line, olfactory epithelium, olfactory bulb, , pallium, subpallium, tract of the habenula commissure, nasal retina.

 


Key Publications

 Haas, P., and Gilmour, D. (2006)
Chemokine signaling mediates self-organizing tissue migration in the zebrafish lateral line.
Developmental Cell. 10(5):673-680.

Folgueira, M., Bayley, P., Navratilova, P., Becker, T.S., Wilson, S.W., and Clarke, J.D. (2012)
Morphogenesis underlying the development of the everted teleost telencephalon.
Neural Development. 7(1):32.

Valdivia, L.E., Young, R.M., Hawkins, T.A., Stickney, H.L., Cavodeassi, F., Schwarz, Q., Pullin, L.M., Villegas, R., Moro, E., Argenton, F., Allende, M.L., and Wilson, S.W. (2011)
Lef1-dependent Wnt/β-catenin signalling drives the proliferative engine that maintains tissue homeostasis during lateral line development.
Development (Cambridge, England). 138(18):3931-3941.

Valentin, G., Haas, P., and Gilmour, D. (2007)
The chemokine SDF1a coordinates tissue migration through the spatially restricted activation of Cxcr7 and Cxcr4b.
Current biology : CB. 17(12):1026-1031.

Picker, A., Cavodeassi, F., Machate, A., Bernauer, S., Hans, S., Abe, G., Kawakami, K., Wilson, S.W., and Brand, M. (2009)
Dynamic coupling of pattern formation and morphogenesis in the developing vertebrate retina.
PLoS Biology. 7(10):e1000214.



Et(CLG-YFP)smb750

Et(CLG-YFP)smb750

About

 synonyms: clgy750, smb750Et.

This enhancer trap line was generated as part of a large enhancer screen performed by the Becker Lab. This enhancer trap transgenic line shows expression of YFP in the  olfactory bulb, telencephalon, pretectum, torus longitudinalis, cerebellum.


External Links:

 ZFIN

Lab or Origin: Becker Lab


Expressed in: 

 olfactory bulb, telencephalon, pretectum, torus longitudinalis, cerebellum.


Key Publications

 Kikuta, H., Laplante, M., Navratilova, P., Komisarczuk, A.Z., Engstrom, P.G., Fredman, D., Akalin, A., Caccamo, M., Sealy, I., Howe, K., Ghislain, J., Pezeron, G., Mourrain, P., Ellingsen, S., Oates, A.C., Thisse, C., Thisse, B., Foucher, I., Adolf, B., Geling, A., Lenhard, B., and Becker, T.S. (2007)
Genomic regulatory blocks encompass multiple neighboring genes and maintain conserved synteny in vertebrates.
Genome research. 17(5):545-555.

Tg(lhx5:GFP)b1205

Tg(lhx5:GFP)b1205

About

 This transgenic line recapitulates the expression of lhx5 transcription factor. Generated by the Westerfield lab using BAC transgenesis. A kaede version of this line is also available. This transgenic has been used to study pathfinding in early telencephalic development (Guo et al., 2012; Zhang et al., 2012, Turner et al., 2019) and also to look at areas afferent to the habenula at later stages of development (Turner et al., 2016).


External Links:

 ZFIN

Lab or Origin: Westerfield Lab


Expressed in: 

 olfactory bulb, pallium, ventral entopeduncular nucleus, preoptic area, prethalamus, stria medularis, tract of the habenula commissure, habenula commisure, habenula neuropil.


Key Publications

Gao, J., Zhang, C., Yang, B., Sun, L., Zhang, C., Westerfield, M., and Peng, G. (2012)
Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish.
PLoS One. 7(5):e36516.

Zhang, C., Gao, J., Zhang, H., Sun, L., and Peng, G. (2012)
Robo2-Slit and Dcc-Netrin1 Coordinate Neuron Axonal Pathfinding within the Embryonic Axon Tracts.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(36):12589-12602.

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30.

Turner, K.J., Hoyle, J., Valdivia, L.E., Cerveny, K.L., Hart, W., Mangoli, M., Geisler, R., Rees, M., Houart, C., Poole, R.J., Wilson, S.W., Gestri, G. (2019)
Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits.
PLoS One. 14:e0211073.

Tg(slc17a6b: DsRed)nns9Tg

Tg(slc17a6b: DsRed)nns9Tg

About

 Slc17a6b is a vesicular glutamate transporter that that mediates the uptake of the excitatory neurotransmitter glutamate into vesicles in the presynaptic terminals of excitatory neurons. This BAC transgenic line from the Yoshihara lab drives the expression of DSRed in glutamatergic neurons. Expression can be seen in many neurons throughout the brain. Strong expression in the dorsal and ventral habenular subnuclei, olfactory bulbs, the pallium and optic tectum


External Links:

 ZFIN

Lab or Origin: Yoshihara Lab


Expressed in: 

 glutamatergic neurons, olfactory epithelium, olfactory bulb, pallium, subpallium, dorsal habenula, ventral habenula, preoptic area, prethalamus, pretectum (AF9), optic tectum, trigeminal sensory ganglion.


Key Publications

 
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009)
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish.
The Journal of neuroscience. 29(15):4756-4767.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum.
Developmental Biology. 343(1-2):1-17.

Et(krt4:EGFP)sqet11

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.


External Links:

 ZFIN

Lab or Origin: Korzh Lab


Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary


Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.


External Links:

 ZFIN

Lab or Origin: Skarmeta Lab


Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum


Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

About

 


The construct used to create this enhancer trap line contains the highly conserved noncoding element  "hs7" from the regulatory region of the human SOX3 gene.


External Links:

 ZFIN

Lab or Origin: Tom Becker Lab


Expressed in: 

 olfactory bulb, subpallium, habenula, AF9 pretectum, optic tectum, cerebellum.


Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.


Tg(1.4dlx5a-dlx6a:GFP)ot1

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

 


Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 


More images

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab


Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

 


Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Tg(slc6a3:EGFP)ot80

Tg(slc6a3:EGFP)ot80

About

The Tg(slc6a3:EGFP)ot80 transgenic line expresses GFP under the control of
cis-regulatory elements of the dopamine transporter (DAT) gene slc6a3.

 


External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab



Transgene expressed in: 

olfactory bulb, subpallium, preoptic region, pretectum, posterior tuberculum, hypothalamus, optic tectum, cerebellum

Key Publications

 

Xi Y1, Yu M, Godoy R, Hatch G, Poitras L, Ekker M.
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19.