Viewing entries tagged
olfactory bulb

Tg(slc17a6b: DsRed)nns9Tg

Tg(slc17a6b: DsRed)nns9Tg

About

 Slc17a6b is a vesicular glutamate transporter that that mediates the uptake of the excitatory neurotransmitter glutamate into vesicles in the presynaptic terminals of excitatory neurons. This BAC transgenic line from the Yoshihara lab drives the expression of DSRed in glutamatergic neurons. Expression can be seen in many neurons throughout the brain. Strong expression in the dorsal and ventral habenular subnuclei, olfactory bulbs, the pallium and optic tectum


External Links:

 ZFIN

Lab or Origin: Yoshihara Lab


Expressed in: 

 glutamatergic neurons, olfactory epithelium, olfactory bulb, pallium, subpallium, dorsal habenula, ventral habenula, preoptic area, prethalamus, pretectum (AF9), optic tectum, trigeminal sensory ganglion.


Key Publications

 
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009)
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish.
The Journal of neuroscience. 29(15):4756-4767.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum.
Developmental Biology. 343(1-2):1-17.

gSA2AZGFF 398A

gSA2AZGFF 398A

About

 a Gal4 enhancer-trap line form the lab of Koichi Kawakami that labels olfactory sensory neurons, hypothalamus and pituitary.


External Links:

 ZFIN

Lab or Origin: Kawakami Lab


Expressed in: 

olfactory sensory neurons, olfactory bulb glomeruli, hypothalamus and pituitary.  


Key Publications

 Asakawa, K., and Kawakami, K. (2009)
The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.
Methods (San Diego, Calif.). 49(3):275-281.

gSAIZGFFD812A

gSAIZGFFD812A

About

 GFP enhancer-trap line from the Kawakami lab that labels the olfactory bulb, pallium, habenula, optic tectum and cerebellum and hindbrain.


External Links:

 ZFIN

Lab or Origin: Kawakami Lab


Expressed in: 

 olfactory bulb, pallium, habenula, optic tectum and cerebellum and hindbrain.


Key Publications

 Asakawa, K., and Kawakami, K. (2009)
The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.
Methods (San Diego, Calif.). 49(3):275-281.

SAGFF(LF)223A

SAGFF(LF)223A

About

 A GFP enhancer trap line from the Kawakami lab that labels the olfactory bulb, pallium, pineal, habenula, pretectum, optic tectum, torus longitudinalis, hypothalamus, pituitary.


External Links:

 ZFIN

Lab or Origin: Kawakami Lab


Expressed in: 

  olfactory bulb, pallium, pineal, habenula, pretectum, optic tectum, torus longitudinalis, hypothalamus, pituitary.


Key Publications

 Asakawa, K., and Kawakami, K. (2009)
The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.
Methods (San Diego, Calif.). 49(3):275-281.

Et(krt4:EGFP)sqet11

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.


External Links:

 ZFIN

Lab or Origin: Korzh Lab


Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary


Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.


External Links:

 ZFIN

Lab or Origin: Skarmeta Lab


Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum


Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

About

 


The construct used to create this enhancer trap line contains the highly conserved noncoding element  "hs7" from the regulatory region of the human SOX3 gene.


External Links:

 ZFIN

Lab or Origin: Tom Becker Lab


Expressed in: 

 olfactory bulb, subpallium, habenula, AF9 pretectum, optic tectum, cerebellum.


Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.


Tg(1.4dlx5a-dlx6a:GFP)ot1

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

 


Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 


More images

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab


Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

 


Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Tg(slc6a3:EGFP)ot80

Tg(slc6a3:EGFP)ot80

About

The Tg(slc6a3:EGFP)ot80 transgenic line expresses GFP under the control of
cis-regulatory elements of the dopamine transporter (DAT) gene slc6a3.

 


External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab



Transgene expressed in: 

olfactory bulb, subpallium, preoptic region, pretectum, posterior tuberculum, hypothalamus, optic tectum, cerebellum

Key Publications

 

Xi Y1, Yu M, Godoy R, Hatch G, Poitras L, Ekker M.
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19.