Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.

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TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP and tubulin lateral view at 4dpf

hindbrain

TG44:EGFP lateral view at 4dpf

hindbrain

TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP dorsal view at 4dpf

hindbrain

TG44:EGFP dorsal view at 4dpf

section through hindbrain showing midline cells

TG44:EGFP and tubulin dorsal view at 4dpf

section through hindbrain showing lateral cells surrounding axon tract.

External Links:

 ZFIN

Lab or Origin: Skarmeta Lab

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Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum

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Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.

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Et(fos:Gal4-VP16)s1026t 4dpf dorsal

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker TOTO-3(grey).

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

Single z-slice through the eye.

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf ventral

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

single z-slice at level of subpallium

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at level of preglomerular complex and hypothlamus.

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice through the posterior tuberculum

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the prethalmus and thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the ventral habenulae

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the dorsal habenulae and pineal organ.

External Links:

 ZFIN

Lab or Origin:

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Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.

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Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Tg(glyt2:GFP)/Tg(slc6a5:GFP)

About

 Synonyms: Tg(slc6a5:GFP), Tg(slc6a5:GFP)cf3, cf3Tg

Previously called glycine transporter 2 now named solute carrier family 6 member 5(slc6a5), this trangenic line labels glycinergic neruons.

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Tg(slc6a5:GFP) dorsal 4dpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 4dpf

Tg(slc6a5:GFP) dorsal 4dpf with tubulin(cyan)

Pineal organ

Tg(slc6a5:GFP) dorsal 4dpf

Pineal organ

Tg(slc6a5:GFP) dorsal 4dpf hindbrain with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 4dpf hindbrain

Tg(slc6a5:GFP) lateral 4dpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 4dpf

Tg(slc6a5:GFP) ventral 4dpf with tubulin(cyan)

Single z-plane

Tg(slc6a5:GFP) ventral 4dpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 48hpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 48hpf

Tg(slc6a5:GFP) lateral 48hpf with tubulin(cyan)

Tg(slc6a5:GFP) lateral 48hpf

Tg(slc6a5:GFP) dorsal 48hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 48hpf

Tg(slc6a5:GFP) lateral 48hpf with tubulin(cyan)

spinal cord

Tg(slc6a5:GFP) lateral 48hpf

spinal cord

Tg(slc6a5:GFP) Lateral 24hpf

Tg(slc6a5:GFP) dorsal 30hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 30hpf

Tg(slc6a5:GFP) lateral 30hpf with tubulin(cyan)

hindbrain/anterior spinal cord

Tg(slc6a5:GFP) lateral 30hpf with tubulin(cyan)

spinal cord

Tg(slc6a5:GFP) lateral 30hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 30hpf with tubulin(cyan)

Tg(slc6a5:GFP) dorsal 30hpf

External Links:

 ZFIN

Lab or Origin: Joe Fetcho Lab

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Expressed in: 

 glycinergic neurons, spinal cord, pineal, hindbrain.

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Key Publications

McLean, D.L., Fan, J., Higashijima, S., Hale, M.E., and Fetcho, J.R. (2007)
A topographic map of recruitment in spinal cord.
Nature. 446(7131):71-75.

Barreiro-Iglesias, A., Mysiak, K.S., Adrio, F., Rodicio, M.C., Becker, C.G., Becker, T., and Anadón, R. (2013)
Distribution of glycinergic neurons in the brain of glycine transporter-2 Tg(glyt2:gfp) transgenic adult zebrafish: Relation with brain-spinal descending systems.
The Journal of comparative neurology. 521(2):389-425.
 

pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.

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4dpf Ventral view of ETvmat2:GFP.

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Ventral view of ETvmat2:GFP with acetylated tubulin(magenta)..

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Lateral view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf Lateral view of ETvmat2:GFP

4dpf dorsal view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf dorsal view of ETvmat2:GFP.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf Ventral view of ETvmat2:GFP

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf dorsal view of ETvmat2:GFP forbrain with acetylated tubulin(cyan) and SV2(red).

5dpf dorsal view of ETvmat2:GFP forebrain.

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455431398154TimeStamp=14:57:11.398 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=6faacf28-fbff-43f2-905a-d9bbdeb24be2

dpf dorsal view of ETvmat2:GFP hindbrain with acetylated tubulin(cyan) and SV2(red).

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455437197463TimeStamp=14:57:17.197 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=201a84ae-28f4-4eba-9aea-281b8840f135

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the olfactory bulb and telencephalon.

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

the telencephalon has been removed to show monoaminergic cells in the torus loingitudinalis and pretectum.

5dpf dorsal view of ETvmat2:GFP hindbrain.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

5dpf Ventral view of ETvmat2:GFP forebrain with acetylated tubulin(cyan).

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Ventral view of ETvmat2:GFP forebrain.

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Lateral view of ETvmat2:GFP diencephalon with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

5dpf Lateral view of ETvmat2:GFP diencephalon

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group

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Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.

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Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.

Tg(3Hsa.SOX3-gata2a:EGFP)bi81Tg

About

 This construct contains the highly conserved noncoding element  "hs5" from the  regulatory region of the human SOX3 gene.

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sox3h5:EGFP 48hpf lateral view

sox3h5:EGFP and tubulin 48hpf lateral view

sox3h5:EGFP dorsal view 48hpf

Dorsal diencephaln showing the midleing pineal organ and asymmetric parapineal on the left.

sox3h5:EGFPand tubulin dorsal view 48hpf

Dorsal diencephaln showing the midleing pineal organ and asymmetric parapineal on the left.

External Links:

 ZFIN

Lab or Origin: Tom Becker Lab

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Expressed in: 

pineal, parapineal, pretectum, thalamus

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Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.

sox3h10:EGFP

About

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sox3h10:EGFP 5dpf lateral

pallial neuron in sox3h10 lateral view at 5dpf

Cilliary marginal zone expression in sox3h10:EGFP

lateral view of retina at 5dpf

Cilliary marginal zone expression in sox3h10:EGFP

lateral view of retina at 5dpf

sox3h10:EGFP and tubulin dorsal view at 5dpf

showing expreession in pineal and habenulae.

sox3h10:EGFP and tubulin dorsal view of hindbrain at 5dpf

sox3h10:EGFP dorsal view of hindbrain at 5dpf

sox3h10:EGFP dorsal diencephalon at 5dpf

sox3h10:EGFP and tubulin Lateral view of hindbrain

5dpf

sox3h10:EGFP and tubulin Lateral view

5dpf

sox3h10:EGFP and tubulin Lateral view 2dpf

sox3h10:EGFP Lateral view at 2dpf

sox3h10:EGFP and tubulin dorsal view 2dpf

sox3h10:EGFP dorsal view 2dpf

External Links:

 ZFIN

Lab or Origin: Tom Becker Lab

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Expressed in: 

 ciliary marginal zone of retina,pallium, pineal, habenula, hindbrain

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Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.