Viewing entries tagged
raphe

pku2Et/ ETvmat2:GFP

pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.


External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group


Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.


Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.

Tg(Pet1:KalTA4)

Tg(Pet1:KalTA4)

About

 This transgenic line was made in the Wilson Lab by Kate Turner, using the pet1 promoter cloned by Lillesaar et al., 2009. The promoter drives KalTA4 and was cloned into a tol2 backbone using the Gateway cloning method (Kwan et al., 2007).

Tg(Pet1:KalTA4) drives expression in serotinergic raphe neurons. In the images below, this driver line was crossed to Tg(14XUAS:mRFP,Xla.Cryg:GFP)tpl2(Auer et al., 2014) and larvae were labelled with anti-RFP and anti-SV2 antibodies to visualise serotinergic raphe neurons and their projections. For a detailed description of the serotinergic system in zebrafish read the excellent Lillesaar et al., 2009 paper. For an overview of the serotinergic system look at our neurotransmitters section.


External Links:

 ZFIN

Lab or Origin: Wilson Lab


Expressed in: 

superior raphe, inferior raphe,  serotinergic neurons


Key Publications

Lillesaar, C., Stigloher, C., Tannhäuser, B., Wullimann, M.F., and Bally-Cuif, L. (2009)
Axonal projections originating from raphe serotonergic neurons in the developing and adult zebrafish, Danio rerio, using transgenics to visualize raphe-specific pet1 expression.
The Journal of comparative neurology. 512(2):158-182.