acetylated tubulin

acetylated tubulin

anti-acetylated tubulin

ABOUT THIS ANTIBODY

anti-acetylated tubulin labels axonal tracts. Please see our separate white matter section for more information about individual tracts and commissures. 

This is a beautiful antibody and like anti-SV2 makes a nice general counterstain when looking at neuroanatomy in transgenic larvae. We also use this antibody extensively to look for any defects in brain development in mutant larvae. 

Mouse monoclonal anti-acetylated tubulin (IgG2b) (alpha-tubulin, Sigma, Cat# T7451, dilution 1:250)

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woodshole_tubulin_labelled.gif
woodshole_tubulin2_labelled.gif

External Links:

 ZFIN:https://zfin.org/ZDB-ATB-081003-6


LABELS THESE BRAIN STRUCTURES:

Axonal tracts. 


KEY PUBLICATIONS

Wilson, S.W., Ross, L.S., Parrett, T., and Easter, S.S. Jr. (1990)
The development of a simple scaffold of axon tracts in the brain of the embryonic zebrafish, Brachydanio rerio. 
Development (Cambridge, England). 108:121-145

Chitnis, A.B. and Kuwada, J.Y. (1990) 
Axonogenesis in the brain of zebrafish embryos. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 10:1892-1905

Karlstrom, R.O., Trowe, T., Klostermann, S., Baier, H., Brand, M., Crawford, A.D., Grunewald, B., Haffter, P., Hoffman, H., Meyer, S.U., Muller, B.K., Richter, S., van Eeden, F.J., Nüsslein-Volhard, C., and Bonhoeffer, F. (1996) 
Zebrafish mutations affecting retinotectal axon pathfinding. 
Development (Cambridge, England). 123:427-438

Calretinin

Calretinin

Rabbit anti-calretinin

ABOUT THIS ANTIBODY

anti-calretinin labels neurons expressing the calcium binding protein calretinin.

Calcium-binding preoteins contribute to calcium homeostasis by buffering intercellular calcium. This antibody labels distinct groups of neurons in the central and peripheral nervous system.
 

Rabbit Polyclonal anti-calretinin (Swant, Cat#CR7697, dilution 1:1000)

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EXTERNAL LINKS:

 ZFIN:https://zfin.org/ZDB-ATB-160726-1
Swant: https://www.swant.com/pdfs/Rabbit_anti_calretinin_7697.pdf


LABELS THESE BRAIN STRUCTURES:

olfactory epithelium, olfactory glomeruli, dorsal telencephalon, ventral telencephalon, entopeduncular nucleus, preoptic area, preglomerular complex, mammillary body, hypothalamus (inferior lobe), torus lateralis, posterior tuberculum, thalamus(anterior thalamic nucleus), pretectum (parvocellular superficial pretectal nucleus), nucleus of the medial longitudinal fascicle. 


KEY PUBLICATIONS

Castro A1, Becerra M, Manso MJ, Anadón R.
Calretinin immunoreactivity in the brain of the zebrafish, Danio rerio: distribution and comparison with some neuropeptides and neurotransmitter-synthesizing enzymes. I. Olfactory organ and forebrain.
J Comp Neurol. 2006 Jan 20;494(3):435-59.

Beretta, C.A., Dross, N., Guglielmi, L., Bankhead, P., Soulika, M., Gutierrez-Triana, J.A., Paolini, A., Poggi, L., Falk, J., Ryu, S., Kapsimali, M., Engel, U., Carl, M. (2017)
Early Commissural Diencephalic Neurons Control Habenular Axon Extension and Targeting.
Current biology : CB. 27(2):270–278.

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits 10:30. https://doi.org/10.3389/fncir.2016.00030

Antibody template

Antibody template

antibody name

ABOUT THIS ANTIBODY

"antibody" labels synaptic neuropil.

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Mouse monoclonal anti-SV2 (IgG1) (DSHB, Cat#AB 2315387, dilution 1:500)

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External Links:

 ZFIN:


LABELS THESE BRAIN STRUCTURES:

 


KEY PUBLICATIONS

 

synaptic vesicle 2 (SV2)

synaptic vesicle 2 (SV2)

Synaptic Vesicle 2 (SV2)

ABOUT THIS ANTIBODY

Synaptic Vesicle 2 labels synaptic neuropil.

We frequently use this antibody in conjunction with  anti-acetylated tubulin (IgG2b, Sigma), which labels beautifully the axonal connections, to aid anatomical orientation in transgenic specimens. In addition to these antibodies being informative from a neuroanatomical perspective, they are also invaluable as a tool for anatomical localisation. These antibodies can be used as a framework to easily compare the expression patterns of different transgenic lines and locate GFP-positive structure in the context of the brain. Both antibodies are mouse monoclonals but can be detected in the same specimen using subtype-specific secondary antibodies.

Mouse monoclonal anti-SV2 (IgG1) (DSHB, Cat#AB 2315387, dilution 1:500)

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External Links:

 ZFIN:https://zfin.org/ZDB-ATB-081201-1


LABELS THESE BRAIN STRUCTURES:

Synaptic neuropil


KEY PUBLICATIONS

Buckley K, Kelly RB (1985)
Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells. 
J Cell Biol 100:1284–1294

Hendricks M, Jesuthasan S (2007)
Asymmetric innervation of the habenula in zebrafish. 
J Comp Neurol 502:611–619

Turner KJ1, Bracewell TG, Hawkins TA.
Anatomical dissection of zebrafish brain development.
Methods Mol Biol. 2014;1082:197-214. doi: 10.1007/978-1-62703-655-9_14.