Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.

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TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP and tubulin lateral view at 4dpf

hindbrain

TG44:EGFP lateral view at 4dpf

hindbrain

TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP dorsal view at 4dpf

hindbrain

TG44:EGFP dorsal view at 4dpf

section through hindbrain showing midline cells

TG44:EGFP and tubulin dorsal view at 4dpf

section through hindbrain showing lateral cells surrounding axon tract.

External Links:

 ZFIN

Lab or Origin: Skarmeta Lab

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Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum

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Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

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Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 

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More images

Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green), SV2(cyan) and tubulin(red) antibodies.

by Monica Folgueira

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP and anti-tubulin(cyan).

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP.

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green).

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and anti-tubulin antibodies.

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and tubulin(cyan) antibodies.

by Kate Turner

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

by KateTurner

Olfactory glomeruli in Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

Frontal view showing the GFP +processes of olfactory bulb interneurons innervating the glomeruli.

Monica Folgueira

24hpf dorsal view of dlx:GFP with tubulin

Tubulin shows the longitudinal axon scaffold formed by this early stage. Several clusters of dlx:GFP + cells are present in the telencephalon, diencephalon, midbrain and hindbrain.

Monica Folgueira

Olfactory bulb Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

single section through a 4dpf olfactory bulb.

Monica Folgueira

3dpf frontal view of dlx:GFP larvae

Monica Folgueira

3dpf dorsal view dlx:GFP

Showing many GFP+ neurons in the optic tectum and cerebellum.

Monica Folgueira

2dpf dlx:GFP ventral view

Shows cells throughout the subpallium and preoptic area and hypothalamic lobes.

Monica Folgueira

3dpf lateral view of dlx:GFP in forebrain

Monica Folgueira

3dpf late view of dlx:GFP hindbrain

showing dense cluster of GFP+ cells in cerebellum and scattered cells in the spinal cord.

Monica Folgueira

3dpf dorsal view of dlx:GFP hindbrain

Monica Folgueira

3dpf frontal section of dlx:GFP subpallium

Monica Folgueira

3dpf thick section of dlx:GFP through midbrain/diencephalon

Shows tectal and pretectal expression.

Monica Folgueira

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab

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Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

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Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Et(fos:Gal4-VP16)s1168t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. Driving Kaede expression in the posterior tuberculum,hypothalamus and cerebellum. There is a very interesting tract labelled that projects between the midbrain tegmentum and posterior tuberculum. The tract skirts the tectal neuropil. The insertion of this transgene is currently unmapped.

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Lateral views of 4dpf s1168t larvae

kaede (green), SV2(magenta), tubulin(cyan)

Ventral views of 4dpf s1168t larvae

kaede(green); SV2(magenta:tubulin(cyan)

Dorsal views of s1168t larvae

kaede (green), SV2(magenta), tubulin(cyan)

External Links:

 ZFIN

Lab or Origin: Baier Lab

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Expressed in: 

tectum, posterior tuberculum, tegmentum, hypothalamus, cerebellum. 

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Key Publications

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Heap, L.A., Goh, C.C., Kassahn, K.S., and Scott, E.K. (2013) Cerebellar output in zebrafish: an analysis of spatial patterns and topography in eurydendroid cell projections. Frontiers in neural circuits. 7:53.

Heap, L.A., Vanwalleghem, G.C., Thompson, A.W., Favre-Bulle, I., Rubinsztein-Dunlop, H., Scott, E.K. (2018) Hypothalamic Projections to the Optic Tectum in Larval Zebrafish. Frontiers in Neuroanatomy. 11:135.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum. Developmental Biology. 343(1-2):1-17. 

Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.

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Et(fos:Gal4-VP16)s1026t 4dpf dorsal

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker TOTO-3(grey).

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

Single z-slice through the eye.

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf ventral

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

single z-slice at level of subpallium

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at level of preglomerular complex and hypothlamus.

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice through the posterior tuberculum

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the prethalmus and thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the ventral habenulae

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the dorsal habenulae and pineal organ.

External Links:

 ZFIN

Lab or Origin:

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Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.

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Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Et(fos:Gal4-VP16)s1137t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It shows dense expression of kaede in the telencephalon and throughout all the habenula subnuclei. The insertion of this transgene is currently unmapped.

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Et(fos:Gal4-VP16)s1137t lateral 4dpf with SV2 and tubulin

Showing broad telencephalic expression. Also expresses GFP in the habenula, posterior tuberculum and hypothalamus.

Et(fos:Gal4-VP16)s1137t lateral 4dpf with SV2

Showing broad telencephalic expression. Also expresses GFP in the habenula, posterior tuberculum and hypothalamus.

Et(fos:Gal4-VP16)s1137t lateral 4dpf

ventral habenula expression inEt(fos:Gal4-VP16)s1137t

dHbm expression in Et(fos:Gal4-VP16)s1137t

dHbl expression in Et(fos:Gal4-VP16)s1137t

External Links:

 ZFIN

Lab or Origin: Baier Laboratory

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Expressed in: 

 telencephalon, posterior tuberculum, habenula, hypothalamus

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Key Publications

Mason, L., Scott, E.K., Staub, W., Finger-Baier, K., and Baier, H. (2009) Expression patterns from GAL4 enhancer trap screen. ZFIN Direct Data Submission. . (http://zfin.org).

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13. 

Tg(gata2:eGFP)bi105

About

The Tg(gata2:eGFP)bi105 enhancer trap transgenic line was generated as part of an enhancer trap screen by the Becker Lab using the Tol2-transposase system. It has GFP expression throughout the pallium, in a small subpallial nucleus, parapineal organ, optic tectum, pretectum, posterior tuberculum and lateral hypothalamus. In the hindbrain, several cell bodies are labelled in the superior raphe and areas of the reticular formation and caudally in areas of the medulla oblongata

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External Links:

 ZFIN

Lab of Origin: Tom Becker Lab

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Transgene expressed in: 

Key Publications

Folgueira, M., Bayley, P., Navratilova, P., Becker, T.S., Wilson, S.W., and Clarke, J.D. (2012)
Morphogenesis underlying the development of the everted teleost telencephalon.
Neural Development. 7(1):32

Ragvin, A., Moro, E., Fredman, D., Navratilova, P., Drivenes, O., Engström, P.G., Alonso, M.E., Mustienes, E.D., Gomez Skarmeta, J.L., Tavares, M.J., Casares, F., Manzanares, M., van Heyningen, V., Molven, A., Njølstad, P.R., Argenton, F., Lenhard, B., and Becker, T.S. (2010)
Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3.
Proceedings of the National Academy of Sciences of the United States of America. 107(2):775-780

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.

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VKET11:EGFP and tubulin dorsal view 4dpf

VKET11:EGFP dorsal view 4dpf

VKET11:EGFP and tubulin ventral view 4dpf

VKET11:EGFP ventral view 4dpf

External Links:

 ZFIN

Lab or Origin: Korzh Lab

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Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary

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Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Et(krt4:EGFP)sqet24

About

 Enhancer trap line from Vladimir Korzh lab that has EGFP expression in the epithalamus, thalamic eminence, hypothalamic lobes, cerebellum, medulla oblongata.

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Et(krt4:EGFP)sqet24 5dpf ventral

Et(krt4:EGFP)sqet24 5dpf ventral with tubulin (magenta)

Et(krt4:EGFP)sqet24 5dpf lateral

Et(krt4:EGFP)sqet24 5dpf lateral with tubulin (magenta)

Et(krt4:EGFP)sqet24 5dpf lateral

Et(krt4:EGFP)sqet24 5dpf lateral with tubulin (magenta)

External Links:

 ZFIN

Lab or Origin: Korzh Lab

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Expressed in: 

epithalamus, thalamic eminence, hypothalamus, cerebellum, medulla oblongata

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Key Publications

Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004)
Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo.
Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

pku2Et/ ETvmat2:GFP

About

 Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP

The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).

This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.

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4dpf Ventral view of ETvmat2:GFP.

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Ventral view of ETvmat2:GFP with acetylated tubulin(magenta)..

Showing monoaminergic neurons in the posterior tuberculum, hypothalamus, ventrolateral serotinergic group and raphe.

4dpf Lateral view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf Lateral view of ETvmat2:GFP

4dpf dorsal view of ETvmat2:GFP with acetylated tubulin(magenta).

4dpf dorsal view of ETvmat2:GFP.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf Ventral view of ETvmat2:GFP

Showing monoaminergic neurons in the posterior tuberculum and hypothalamus.

5dpf dorsal view of ETvmat2:GFP forbrain with acetylated tubulin(cyan) and SV2(red).

5dpf dorsal view of ETvmat2:GFP forebrain.

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455431398154TimeStamp=14:57:11.398 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=6faacf28-fbff-43f2-905a-d9bbdeb24be2

dpf dorsal view of ETvmat2:GFP hindbrain with acetylated tubulin(cyan) and SV2(red).

[Improvision Data]ImageName=TimeStampMicroSeconds=3319455437197463TimeStamp=14:57:17.197 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicrons=1ZCalibrationMicrons=1TotalChannels=1TotalTimepoints=1TotalZPlanes=1Software=Volocity 4.2.0SampleUUID=201a84ae-28f4-4eba-9aea-281b8840f135

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the olfactory bulb and telencephalon.

5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

the telencephalon has been removed to show monoaminergic cells in the torus loingitudinalis and pretectum.

5dpf dorsal view of ETvmat2:GFP hindbrain.

5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).

5dpf Ventral view of ETvmat2:GFP forebrain with acetylated tubulin(cyan).

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Ventral view of ETvmat2:GFP forebrain.

Showing monoaminergic neurons in the olfactory bulb and ventral telencephalon and preoptic area.

5dpf Lateral view of ETvmat2:GFP diencephalon with acetylated tubulin(cyan) and SV2(red).

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

5dpf Lateral view of ETvmat2:GFP diencephalon

Showing monoaminergic neurons in the pretectum and GFP+ processes in the optic tectumand throughout the prethalamus, thalamus and thalamic emminence.

External Links:

 ZFIN

Lab or Origin: PKU Zebrafish Functional Genomics Group

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Expressed in: 

 monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.

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Key Publications

Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92. 

Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.

Tg(slc6a3:EGFP)ot80

About

The Tg(slc6a3:EGFP)ot80 transgenic line expresses GFP under the control of
cis-regulatory elements of the dopamine transporter (DAT) gene slc6a3.

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External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab

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Tg(slc6a3:EGFP) 3dpf Dorsal View SV2 TUB

Tg(slc6a3:EGFP) 3dpf Dorsal View SV2

Tg(slc6a3:EGFP) 3dpf Dorsal View

Tg(slc6a3:EGFP) 3dpf Lateral View SV2 TUB

Tg(slc6a3:EGFP) 3dpf Lateral View SV2

Tg(slc6a3:EGFP) 3dpf Lateral View

Tg(slc6a3:EGFP) 3dpf Ventral View SV2 TUB

Tg(slc6a3:EGFP) 3dpf Ventral View SV2

Tg(slc6a3:EGFP) 3dpf Ventral View

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Transgene expressed in: 

olfactory bulb, subpallium, preoptic region, pretectum, posterior tuberculum, hypothalamus, optic tectum, cerebellum

Key Publications

Xi Y1, Yu M, Godoy R, Hatch G, Poitras L, Ekker M.
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19.