Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.

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TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

TG44:EGFP and tubulin lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP lateral view at 4dpf

midbrain and hindbrain

TG44:EGFP and tubulin lateral view at 4dpf

hindbrain

TG44:EGFP lateral view at 4dpf

hindbrain

TG44:EGFP and tubulin dorsal view at 4dpf

TG44:EGFP dorsal view at 4dpf

hindbrain

TG44:EGFP dorsal view at 4dpf

section through hindbrain showing midline cells

TG44:EGFP and tubulin dorsal view at 4dpf

section through hindbrain showing lateral cells surrounding axon tract.

External Links:

 ZFIN

Lab or Origin: Skarmeta Lab

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Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum

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Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

Tg(atoh7 :gapRFP)cu2Tg

About

 atonal bHLH transcription factor 7 is turned on in retinal ganglion cells as they transition from proliferating neuroblast to differentiated neuron. The atoh7 promoter in this transgenic line drives the expression of a membrane tagged version of RFP so the axons of the RGCs are also labelled so the optic nerve and tract projecting from the retina to the optic tectum can be distinguished.

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External Links:

 ZFIN

Lab or Origin: Bill Harris Lab

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Expressed in: 

Retinal ganglion cells, cilliary marginal zone, retina, optic nerve. optic tract, optic tectum.  

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Key Publications

 Zolessi, F.R., Poggi, L., Wilkinson, C.J., Chien, C.B., and Harris, W.A. (2006)
Polarization and orientation of retinal ganglion cells in vivo.
Neural Development. 1:2.

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

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Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 

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More images

Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green), SV2(cyan) and tubulin(red) antibodies.

by Monica Folgueira

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP and anti-tubulin(cyan).

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Lateral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with anti-GFP.

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green).

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Ventral view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and anti-tubulin antibodies.

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae labelled with anti-GFP(green) and tubulin(cyan) antibodies.

by Kate Turner

Dorsal view of a 4dpf Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae

by KateTurner

Olfactory glomeruli in Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

Frontal view showing the GFP +processes of olfactory bulb interneurons innervating the glomeruli.

Monica Folgueira

24hpf dorsal view of dlx:GFP with tubulin

Tubulin shows the longitudinal axon scaffold formed by this early stage. Several clusters of dlx:GFP + cells are present in the telencephalon, diencephalon, midbrain and hindbrain.

Monica Folgueira

Olfactory bulb Tg(1.4dlx5a-dlx6a:GFP)ot1 labelled with GFP, tubulin and sv2

single section through a 4dpf olfactory bulb.

Monica Folgueira

3dpf frontal view of dlx:GFP larvae

Monica Folgueira

3dpf dorsal view dlx:GFP

Showing many GFP+ neurons in the optic tectum and cerebellum.

Monica Folgueira

2dpf dlx:GFP ventral view

Shows cells throughout the subpallium and preoptic area and hypothalamic lobes.

Monica Folgueira

3dpf lateral view of dlx:GFP in forebrain

Monica Folgueira

3dpf late view of dlx:GFP hindbrain

showing dense cluster of GFP+ cells in cerebellum and scattered cells in the spinal cord.

Monica Folgueira

3dpf dorsal view of dlx:GFP hindbrain

Monica Folgueira

3dpf frontal section of dlx:GFP subpallium

Monica Folgueira

3dpf thick section of dlx:GFP through midbrain/diencephalon

Shows tectal and pretectal expression.

Monica Folgueira

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab

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Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

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Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Et(fos:Gal4-VP16)s1168t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. Driving Kaede expression in the posterior tuberculum,hypothalamus and cerebellum. There is a very interesting tract labelled that projects between the midbrain tegmentum and posterior tuberculum. The tract skirts the tectal neuropil. The insertion of this transgene is currently unmapped.

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Lateral views of 4dpf s1168t larvae

kaede (green), SV2(magenta), tubulin(cyan)

Ventral views of 4dpf s1168t larvae

kaede(green); SV2(magenta:tubulin(cyan)

Dorsal views of s1168t larvae

kaede (green), SV2(magenta), tubulin(cyan)

External Links:

 ZFIN

Lab or Origin: Baier Lab

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Expressed in: 

tectum, posterior tuberculum, tegmentum, hypothalamus, cerebellum. 

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Key Publications

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Heap, L.A., Goh, C.C., Kassahn, K.S., and Scott, E.K. (2013) Cerebellar output in zebrafish: an analysis of spatial patterns and topography in eurydendroid cell projections. Frontiers in neural circuits. 7:53.

Heap, L.A., Vanwalleghem, G.C., Thompson, A.W., Favre-Bulle, I., Rubinsztein-Dunlop, H., Scott, E.K. (2018) Hypothalamic Projections to the Optic Tectum in Larval Zebrafish. Frontiers in Neuroanatomy. 11:135.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum. Developmental Biology. 343(1-2):1-17. 

Et(fos:Gal4-VP16)s1026t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It labels prethalamus, thalamus and posterior tubercular regions. It also has retinal, habenular, pineal, optic tectum and subpallial expression.

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Et(fos:Gal4-VP16)s1026t 4dpf dorsal

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker TOTO-3(grey).

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

Single z-slice through the eye.

Et(fos:Gal4-VP16)s1026t 4dpf ventral with nuclear marker(grey).

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf ventral

single z slice through the eye

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

single z-slice at level of subpallium

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at level of preglomerular complex and hypothlamus.

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice through the posterior tuberculum

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the prethalmus and thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the thalamus

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the ventral habenulae

Et(fos:Gal4-VP16)s1026t 4dpf dorsal with nuclear marker(grey).

Single z-slice at the level of the dorsal habenulae and pineal organ.

External Links:

 ZFIN

Lab or Origin:

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Expressed in: 

 subpallium, habenulae, pineal, emminentia thalami, prethalamus, thalamus, posterior tuberculum, retina, optic tectum, hypothalamus, preglomerular complex.

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Key Publications

 Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13.

Tg(gata2:eGFP)bi105

About

The Tg(gata2:eGFP)bi105 enhancer trap transgenic line was generated as part of an enhancer trap screen by the Becker Lab using the Tol2-transposase system. It has GFP expression throughout the pallium, in a small subpallial nucleus, parapineal organ, optic tectum, pretectum, posterior tuberculum and lateral hypothalamus. In the hindbrain, several cell bodies are labelled in the superior raphe and areas of the reticular formation and caudally in areas of the medulla oblongata

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External Links:

 ZFIN

Lab of Origin: Tom Becker Lab

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Transgene expressed in: 

Key Publications

Folgueira, M., Bayley, P., Navratilova, P., Becker, T.S., Wilson, S.W., and Clarke, J.D. (2012)
Morphogenesis underlying the development of the everted teleost telencephalon.
Neural Development. 7(1):32

Ragvin, A., Moro, E., Fredman, D., Navratilova, P., Drivenes, O., Engström, P.G., Alonso, M.E., Mustienes, E.D., Gomez Skarmeta, J.L., Tavares, M.J., Casares, F., Manzanares, M., van Heyningen, V., Molven, A., Njølstad, P.R., Argenton, F., Lenhard, B., and Becker, T.S. (2010)
Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3.
Proceedings of the National Academy of Sciences of the United States of America. 107(2):775-780

Turner, K.J., Hawkins, T.A., Yáñez, J., Anadón, R., Wilson, S.W., Folgueira, M. (2016)
Afferent Connectivity of the Zebrafish Habenulae.
Frontiers in neural circuits. 10:30

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.

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VKET11:EGFP and tubulin dorsal view 4dpf

VKET11:EGFP dorsal view 4dpf

VKET11:EGFP and tubulin ventral view 4dpf

VKET11:EGFP ventral view 4dpf

External Links:

 ZFIN

Lab or Origin: Korzh Lab

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Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary

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Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Tg(pou4f1-hsp70l:GFP)

About

Tg(pou4f1-hsp70l:GFP) formally Tg(brn3a-hsp70:GFP) expresses green fluorescent protein (GFP) under the control of enhancer elements of brn3a, a POU-domain transcription-factor-encoding gene expressed in the habenula, retina, optic tectum, torus semicircularis and cranial sensory ganglia including the neuromasts of the lateral line and inner ear.

GFP is expressed in retinal ganglion cells and amacrine cells. Projections from RGCs can be seen traversing the optic nerve and innervating the superficial neuropil layer of the optic tectum. In addition, there were neurons expressing GFP in the deeper layers of the tectum. Given that the tectum sends motor outputs from the deeper layers it is likely that these neurons were involved in sending motor outputs to the hindbrain.

The tectobulbar tract descends from the deeper layers of the optic tectum ipsilaterally to connect with the reticulospinal neurons in the hindbrain (Sato et al., 2007).

This transgenic expresses GFP in the dorsal medial habenula subnuclei. Projections from dHbM neurons to the IPN can be seen traversing the fasiculus retroflexus and terminating in the Interpeduncular nuclei. Habenula axons exhibit a spiralling terminal morphology when innervating the IPN (Aizawa et al., 2005).

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External Links:

 ZFIN

Lab of Origin: Hitoshi Okamoto Lab

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brn3a_lat_4dpf.png

Lateral view of a 4dpf Tg(pou4f1:hsp70l:GFP) embryo labelled with anti-GFP and anti-tubulin.

brn3a_4dpf_lat_noeye.png

Lateral view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. Eye has been removed to show the optic nerve ascending to the optic tectum.

brn3a_4dpf_ventral.png

view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP. Showing the dicussation of the optic nerve at the optic chiasm.

brn3a_4dpf_vent.png

view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. Showing the dicussation of the optic nerve at the optic chiasm.

brn3a_60hpf_dorsal.png

Dorsal view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. At this stage of development, GFP + dorsal habenula neurons have increased in number. Retinal ganglion cell axons innervate the superficial layer of the optic tectum. GFP positive cells are also present in the deeper tectal layers.

brn3a_60hpf_dorsal_IPN.png

Dorsal view of a 60hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP. This z-projection is cropped to show the habenula afferent terminals spiralling innervation pattern in the IPN.

brn3a_60hpf_lat.png

Lateral view of a 60hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. 

brn3a_36hpf_lateral.png

Lateral view of a 36hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. 

brn3a_48hpf_dorsal.png

Lateral view of a 48hpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. 

brn3a_4dpf_cranial ganglia.png

dorsal view of a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-tubulin. Showing hair cells of the inner ear and neuromasts of the lateral line.

Brn3a_4dpf_habenula.png

Dorsal view of the habenula in a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP. This transgene is expressed in the medial subnucleus of the dorsal habenula.

brn3a_4dpf_habenula_dapi.png

Dorsal view of the habenula in a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and DAPI. This transgene is expressed in the medial subnucleus of the dorsal habenula.

brn3a_4dpf_habenula_SV2.png

Dorsal view of the habenula in a 4dpf Tg(pou4f1:hsp70l:GFP) larvae labelled with anti-GFP and anti-SV2. This transgene is expressed in the medial subnucleus of the dorsal habenula.

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Transgene expressed in: 

dorsal habenula, retina, optic tectum, tecto-bulbar tract, fasiculus retroflexus, neuromasts of lateral line, cranial nerve ganglia, hair cells of inner ear.

Key Publications

Sato, T., Hamaoka, T., Aizawa, H., Hosoya, T., and Okamoto, H. (2007)
Genetic single-cell mosaic analysis implicates ephrinB2 reverse signaling in projections from the posterior tectum to the hindbrain in zebrafish.
The Journal of Neuroscience 27(20):5271-5279.

Aizawa, H., Bianco, I.H., Hamaoka, T., Miyashita, T., Uemura, O., Concha, M.L., Russell, C., Wilson, S.W., and Okamoto, H. (2005)
Laterotopic Representation of Left-Right Information onto the Dorso-Ventral Axis of a Zebrafish Midbrain Target Nucleus.
Current biology. 15(3):238-243.

deCarvalho, T.N., Subedi, A., Rock, J., Harfe, B.D., Thisse, C., Thisse, B., Halpern, M.E., Hong, E. (2014) Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish.
Genesis (New York, N.Y. : 2000). 52(6):636-55.

Tg(slc6a3:EGFP)ot80

About

The Tg(slc6a3:EGFP)ot80 transgenic line expresses GFP under the control of
cis-regulatory elements of the dopamine transporter (DAT) gene slc6a3.

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External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab

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Tg(slc6a3:EGFP) 3dpf Dorsal View SV2 TUB

Tg(slc6a3:EGFP) 3dpf Dorsal View SV2

Tg(slc6a3:EGFP) 3dpf Dorsal View

Tg(slc6a3:EGFP) 3dpf Lateral View SV2 TUB

Tg(slc6a3:EGFP) 3dpf Lateral View SV2

Tg(slc6a3:EGFP) 3dpf Lateral View

Tg(slc6a3:EGFP) 3dpf Ventral View SV2 TUB

Tg(slc6a3:EGFP) 3dpf Ventral View SV2

Tg(slc6a3:EGFP) 3dpf Ventral View

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Transgene expressed in: 

olfactory bulb, subpallium, preoptic region, pretectum, posterior tuberculum, hypothalamus, optic tectum, cerebellum

Key Publications

Xi Y1, Yu M, Godoy R, Hatch G, Poitras L, Ekker M.
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19.

Tg(slc17a6b: DsRed)nns9Tg

About

 Slc17a6b is a vesicular glutamate transporter that that mediates the uptake of the excitatory neurotransmitter glutamate into vesicles in the presynaptic terminals of excitatory neurons. This BAC transgenic line from the Yoshihara lab drives the expression of DSRed in glutamatergic neurons. Expression can be seen in many neurons throughout the brain. Strong expression in the dorsal and ventral habenular subnuclei, olfactory bulbs, the pallium and optic tectum

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Tg(slc17a6b:DsRed), tubulin and SV2 24hpf frontal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin and SV2 hpf dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin and SV2 48hpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 48hpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green

Tg(slc17a6b:DsRed) 48hpf Lateral

Tg(slc17a6b:DsRed), tubulin, SV2 48hpf Dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed) 48hpf Dorsal

Tg(slc17a6b:DsRed), tubulin 4dpf Dorsal

Tg(slc17a6b:DsRed) in cyan; Tubulin in green;

Tg(slc17a6b:DsRed) 4dpf Dorsal

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed) 4dpf Lateral

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed) and tubulin 4dpf Lateral

Tg(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed) 4dpf Lateral

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Ventral

g(slc17a6b:DsRed) in cyan; Tubulin in green; SV2 in magenta.

Tg(slc17a6b:DsRed), tubulin 4dpf Ventral

g(slc17a6b:DsRed) in cyan; Tubulin in green.

Tg(slc17a6b:DsRed), tubulin and SV2 4dpf Ventral

External Links:

 ZFIN

Lab or Origin: Yoshihara Lab

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Expressed in: 

 glutamatergic neurons, olfactory epithelium, olfactory bulb, pallium, subpallium, dorsal habenula, ventral habenula, preoptic area, prethalamus, pretectum (AF9), optic tectum, trigeminal sensory ganglion.

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Key Publications

 
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009)
From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish.
The Journal of neuroscience. 29(15):4756-4767.

Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M. (2010) Proneural gene-linked neurogenesis in zebrafish cerebellum.
Developmental Biology. 343(1-2):1-17.

Tg5(Hsa.SOX3-gata2a:EGFP)bi85Tg

About

The construct used to create this enhancer trap line contains the highly conserved noncoding element  "hs7" from the regulatory region of the human SOX3 gene.

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sox3h7:EGFP with SV2 lateral view 4dpf

sox3h7:EGFP lateral view 4dpf

External Links:

 ZFIN

Lab or Origin: Tom Becker Lab

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Expressed in: 

 olfactory bulb, subpallium, habenula, AF9 pretectum, optic tectum, cerebellum.

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Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.

io005Tg/Tg(Xleomes:GFP)io005

About

 Synonyms: Tg(Xleomes:GFP)io005, tg(Xeom:GFP)

The tg(Xeom:GFP) transgenic lines were made in the Mione lab and used to study the dorsal to ventral migration of the paraseptal neurons in the subpallium.

“GFP-expressing cells appear around 28 hpf in the telencephalon of tg(Xeom:GFP) transgenic embryos. Paired groups of GFP-expressing cells appear in the lateral region of the telencephalon more or less at the level of the olfactory placode, at the telencephalic/diencephalic border and in the midbrain tegmentum.
We describe the migration of the telencephalic group: these cells originate from the corresponding ventricular zone, they first move towards the lateral side and then proceed rapidly towards the ventral telencephalon. Most of the GFP+ cells will congregate at the level of the septal area just rostral to the anterior commissure The 3 cell groups are interconnected through the anterior commissure and the middle forebrain bundle.” (Mione et al., 2008).

“Telencephalic Xeom:GFP- expressing cells probably correspond to the eomes/tbr1- expressing cells located in the ventral telencephalon of zebrafish [Mione et al., 2001] and of many other vertebrates [Brox et al., 2004; Puelles et al., 2000]. The observation that these cells originate from a dorsal telencephalic position and undergo an extensive migration towards the ventral telencephalon and diencephalon, accompanying the growth of the MOT and MFB, suggests that they may play pioneer roles on the formation of these major axon tracts. “(Mione et al., 2008).

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xeom:GFP 60hpf ventral

xeom:GFP 60hpf ventral and tubulin(magenta)

xeom:GFP 60hpf dorsal

xeom:GFP 60hpf dorsal and tubulin(magenta)

xeom:GFP 3dpf dorsal with tubulin(magenta)

xeom:GFP 3dpf dorsal

xeom:GFP 5dpf dorsal with tubulin(magenta)

xeom:GFP 3dpf lateral and tubulin (magenta)

xeom:GFP 60hpf lateral

xeom:GFP 60hpf lateral with tubulin(magenta)

xeom:GFP 3dpf lateral

xeom:GFP 3dpf dorsal

xeom:GFP 3dpf dorsal with tubulin(magenta)

xeom:GFP 5dpf lateral with tubulin (magenta)

xeom:GFP 5dpf lateral

xeom:GFP 3dpf lateral

xeom:GFP 3dpf lateral with tubulin (magenta)

xeom:GFP 3dpf lateral

xeom:GFP 2dpf lateral with tubulin (magenta)

xeom:GFP 2dpf lateral

xeom:GFP 2dpf frontal with tubulin (magenta)

xeom:GFP 2dpf frontal

xeom:GFP 40dpf dorsal

xeom:GFP 4dpf dorsal with tubulin(magenta).

xeom:GFP 4dpf lateral

xeom:GFP 4dpf lateral with tubulin(magenta)

xeom:GFP 4dpf ventral

xeom:GFP 4dpf ventral with tubulin(magenta).

xeom:GFP 36hpf lateral

xeom:GFP 36hpf lateral with tubulin(magenta).

xeom:GFP 36hpf lateral

xeom:GFP 36hpf lateral with tubulin(magenta).

xeom:GFP 36hpf frontal

xeom:GFP 36hpf frontal with tubulin(magenta)

External Links:

 ZFIN

Lab or Origin: Mione Lab

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Expressed in: 

 septal region, subpallium, entopeduncular nucleus, thalamic emminence, midbrain tegmentum, optic tectum, cerebellum.

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Key Publications

Mione, M., Baldessari, D., Deflorian, G., Nappo, G., and Santoriello, C. (2008)
How neuronal migration contributes to the morphogenesis of the CNS: insights from the zebrafish. Developmental neuroscience. 30(1-3):65-81.

Mione M, Shanmugalingam S, Kimelman D, Griffin K (2001):
Overlapping expression of zebrafish T-brain-1 and eomesodermin during forebrain development.
Mech Dev 100: 93–97.

Puelles L, Rubenstein JL (2003)
Forebrain gene expression domains and the evolving prosomeric model.
Trends Neurosci 26:469–476.

Puelles L, Kuwana E, Puelles E, Bulfone A, Shi- mamura K, Keleher J, Smiga S, Rubenstein JL (2000)
Pallial and subpallial derivatives in the embryonic chick and mouse telencephalon, traced by the expression of the genes Dlx-2, Emx-1, Nkx-2.1, Pax-6, and Tbr-1.
J Comp Neurol 424:409–438.