Viewing entries tagged
telencephalon

Tg(oxt:EGFP)

Tg(oxt:EGFP)

About

 This transgenic construct drives GFP expression in oxytocin expressing neurons in the neuro-secretary pre-optic area(NPO) part of the neuroendocrine system.


External Links:

 ZFIN

Lab or Origin: Gil Levkowitz Lab


Expressed in: 

preoptic area, neurosecretory preoptic area (NPO).  


Key Publications

Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G. (2011)
The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary.
Developmental Cell. 21(4):642-654.


Machluf, Y., Gutnick, A., and Levkowitz, G. (2011)
Development of the zebrafish hypothalamus.
Annals of the New York Academy of Sciences. 1220(1):93-105.

Et(krt4:EGFP)sqet11

Et(krt4:EGFP)sqet11

About

This enhancer trap construct carries the EGFP gene controlled by a partial epithelial promoter from the keratin8 gene.


External Links:

 ZFIN

Lab or Origin: Korzh Lab


Expressed in: 

 olfactory bulb, subpllium, parapineal, torus longitudinlis, optic tectum, posterior tuberal region, hypothalamus, pituitary


Key Publications

 Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. (2004) Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental dynamics : an official publication of the American Association of Anatomists. 231(2):449-459.

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

Tg44(FRT-Xla.Actc1:DsRed-GAB-FRT,LOXP-Hsa.IRX3-LOXP-gata2a:EGFP-5HS4)upo73Tg

About

 Construct contains the highly conserved noncoding sequence C99 from the human IRXB cluster, containing the genes IRX3, IRX5, and IRX6.  

TG44 is an enhancer trap line from the Skarmeta Lab. Generated as part of a study looking at the transcriptional regulatory properties of highly-conserved noncoding elements on chromosome 16.


External Links:

 ZFIN

Lab or Origin: Skarmeta Lab


Expressed in: 

olfactory bulb, pineal, torus longitudinalis, optic tectum, hypothalamus, hindbrain, cerebellum


Key Publications

 Royo JL, Hidalgo C, Roncero Y, Seda MA, Akalin A, Lenhard B, et al. (2011) Dissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regions. PLoS ONE 6(9): e24824. https://doi.org/10.1371/journal.pone.0024824

sox3h10:EGFP

sox3h10:EGFP

About

 


External Links:

 ZFIN

Lab or Origin: Tom Becker Lab


Expressed in: 

 ciliary marginal zone of retina,pallium, pineal, habenula, hindbrain


Key Publications

 Navratilova, P., Fredman, D., Hawkins, T.A., Turner, K., Lenhard, B., and Becker, T.S. (2009) Systematic human/zebrafish comparative identification of cis-regulatory activity around vertebrate developmental transcription factor genes. Developmental Biology. 327(2):526-540.

Tg(-2.7shh:GFP)

Tg(-2.7shh:GFP)

About

The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish  two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.


All images of shh:GFP were produced by Monica Folgueira.

External Links:

 ZFIN

Lab or Origin: Nüsslein-Volhard, Christiane


Expressed in: 

basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.

 


Key Publications

 Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.

Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176

Et(fos:Gal4-VP16)s1137t

Et(fos:Gal4-VP16)s1137t

About

 This transgenic originates from Herwig Baier’s laboratory and is one of many enhancer trap Gal4 lines created by them. It shows dense expression of kaede in the telencephalon and throughout all the habenula subnuclei. The insertion of this transgene is currently unmapped.


External Links:

 ZFIN

Lab or Origin: Baier Laboratory


Expressed in: 

 telencephalon, posterior tuberculum, habenula, hypothalamus


Key Publications

Mason, L., Scott, E.K., Staub, W., Finger-Baier, K., and Baier, H. (2009) Expression patterns from GAL4 enhancer trap screen. ZFIN Direct Data Submission. . (http://zfin.org).

Scott, E.K., and Baier, H. (2009) The cellular architecture of the larval zebrafish tectum, as revealed by gal4 enhancer trap lines. Frontiers in neural circuits. 3:13. 

Tg(1.4dlx5a-dlx6a:GFP)ot1

Tg(1.4dlx5a-dlx6a:GFP)ot1

About

Tg(1.4dlx5a-dlx6a:GFP)ot1 larvae express GFP in subpallial neurons γ-aminobutyric acid (GABA)-expressing neurons. Several other brain regions also show GFP expression in this transgenic line such as the optic tectum and cerebellum.
 

 Dlx homeobox genes play essential roles in the differentiation, migration and survival of subpallial precursor cells that will later give rise to diverse subtypes of γ-aminobutyric acid (GABA)-expressing neurons. They also participate in the regulation of the Gad genes encoding the enzymes necessary for GABA synthesis (Yu et al., 2011).

 


Mouse over the different areas of GFP expression in the interactive images below to see the name of the brain area. 


More images

External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab


Expressed in: 

olfactory bulb, subpallium, pallium, preoptic area, prethalamus, posterior tuberculum, hypothalamus, optic tectum, cerebellum.

 


Key Publications

Zerucha, T., Stuhmer, T., Hatch, G., Park, B.K., Long, Q., Yu, G., Gambarotta, A., Schultz, J.R., Rubenstein, J.L., and Ekker, M. (2000) 
A highly conserved enhancer in the Dlx5/Dlx6 intergenic region is the site of cross-regulatory interactions between dlx genes in the embryonic forebrain. 
The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(2):709-721.

Yu, M., Xi, Y., Pollack, J., Debiais-Thibaud, M., Macdonald, R.B., and Ekker, M. (2011) 
Activity of dlx5a/dlx6a regulatory elements during zebrafish GABAergic neuron development. 
Int. J. Dev. Neurosci.. 29(7):681-91.

Tg(slc6a3:EGFP)ot80

Tg(slc6a3:EGFP)ot80

About

The Tg(slc6a3:EGFP)ot80 transgenic line expresses GFP under the control of
cis-regulatory elements of the dopamine transporter (DAT) gene slc6a3.

 


External Links:

 ZFIN

Lab of Origin: Mark Ekker Lab



Transgene expressed in: 

olfactory bulb, subpallium, preoptic region, pretectum, posterior tuberculum, hypothalamus, optic tectum, cerebellum

Key Publications

 

Xi Y1, Yu M, Godoy R, Hatch G, Poitras L, Ekker M.
Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon.
Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19.