Viewing entries tagged
Killifish_other cellular marker

4C4

4C4

4C4

ABOUT THIS ANTIBODY

Anti-4C4 labels microglia

4C4 antibody has been found to bind to microglia in the zebrafish retina (Raymond et al., 2006). In the killifish 4C4 labelled Müller glia in the ganglion cell layer, particularly the endfeet. Further, staining was found in the photoreceptors.

Mouse Monoclonal anti-4C4 (Merck, Cat#92092321, dilution 1:150)

image

by Eva-Maria Breitenbach

Section of 4 mpf male killifish sodium citrate antigen retrieval anti-4C4 in magenta and DAPI in blue

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

labels these retinal cell types

Microglia

key publications

Raymond PA, Barthel LK, Bernardos RL and Perkowski JJ. 2006. Molecular characterization of retinal stem cells and their niches in adult zebrafish. BMC Developmental Biology. 6:1-17.

Carbonic Anhydrase 1 (CA1)

Carbonic Anhydrase 1 (CA1)

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Rabbit Polyclonal anti-CA1 (Abcam, Cat#ab108367, dilution 1:200)

image

by Ryan MacDonald

labels these retinal cell types

Microglia

Caspase-3

Caspase-3

Caspase-3

ABOUT THIS ANTIBODY

Anti-Caspase-3 labels apoptosis

Caspase-3 antibody, caspases, are crucial mediators in apoptosis and caspase-3 is activated during death protease. Its antibody labels apoptosis in mice (Gashegu et al., 2007). In the killifish, we found two cells stained in the ganglion cell layer (white arrow) and some staining in the outer plexiform layer (yellow arrow).

Rabbit Polyclonal anti-Caspase-3 (BD Biosciences, Cat#559565, dilution 1:200)

image

by Eva-Maria Breitenbach

Section of 4 mpf male killifish sodium citrate antigen retrieval anti-Caspase-3 in green and DAPI in blue

labels these retinal cell types

Cells undergoing apoptosis

key publications

Gashegu J, Ladha R, Vanmuylder N, Philippson C, Bremer F, Rooze M and Louryan S. 2007. HSP110, caspase‐3 and‐9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all‐trans retinoic acid or irradiation during early mouse eye development. Journal of Anatomy. 210:532-541.

Glial fibrillary acidic protein (Gfap)

Glial fibrillary acidic protein (Gfap)

Glial fibrillary acidic protein (Gfap)

ABOUT THIS ANTIBODY

Anti-Gfap labels gliosis

Glial fibrillary acidic protein (Gfap) is an intermediate filament protein found in Müller glia and is upregulated under retinal stress. It has been found to label retinal gliosis in zebrafish (Dhakal et al., 2021). Gfap antibody showed a strong staining along the Müller glia in the killifish.

Rabbit polyclonal anti-Gfap (Proteintech, Cat#16825-1-AP, dilution 1:100)

image

by Eva-Maria Breitenbach

Section of 2 mpf female killifish sodium citrate antigen retrieval anti-Gfap (green) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

labels these retinal cell types

Gliosis

key publications

Dhakal S, Rotem-Bamberger S, Sejd JR, Sebbagh M, Ronin N, Frey RA, Beitsch M, Batty M, Taler K, Blackerby JF and Inbal A. 2021. Selective requirements for vascular endothelial cells and circulating factors in the regulation of retinal neurogenesis. Frontiers in Cell and Developmental Biology. 9:1-23.

Glial fibrillary acidic protein (Gfap) [ZRF-1]

Glial fibrillary acidic protein (Gfap) [ZRF-1]

ABOUT THIS ANTIBODY

Anti-Gfap [ZPR-1] labels gliosis

Glial fibrillary acidic protein (Gfap) is an intermediate filament protein found in Müller glia and is upregulated under retinal stress. It has been found to label retinal gliosis in zebrafish (Dhakal et al., 2021). Gfap antibody showed a strong staining along the Müller glia in the killifish.

Mouse monoclonal anti-Gfap [ZPR-1] (ZIRC, Cat#AB_10013806, dilution 1:50)

image

by Ryan MacDonald

labels these retinal cell types

Gliosis

key publications

Dhakal S, Rotem-Bamberger S, Sejd JR, Sebbagh M, Ronin N, Frey RA, Beitsch M, Batty M, Taler K, Blackerby JF and Inbal A. 2021. Selective requirements for vascular endothelial cells and circulating factors in the regulation of retinal neurogenesis. Frontiers in Cell and Developmental Biology. 9:1-23.

Proliferating cell nuclear antigen (PCNA)

Proliferating cell nuclear antigen (PCNA)

Proliferating cell nuclear antigen (PCNA)

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Anti-PCNA labels proliferating cells

Proliferating cell nuclear antigen (PCNA) antibody which binds to the PCNA p36 protein highly expressed in proliferating cells and found to stain proliferating cells in the zebrafish (Mazzitelli-Fuentes et al., 2022). In the killifish it stained many cells in the ganglion cell and outer nuclear layer. Additionally, it labels cell walls in the inner nuclear layer.

Mouse monoclonal anti-PCNA (Santa Cruz, Cat#sc-56, dilution 1:250)

image

by Eva-Maria Breitenbach

Section of 2 mpf female killifish sodium citrate antigen retrieval anti-PCNA (magenta) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

labels these retinal cell types

Proliferating cells

key publications

Mazzitelli-Fuentes LS, Román FR, Elías JRC, Deleglise EB and Mongiat LA. 2022. Spatial Learning Promotes Adult Neurogenesis in Specific Regions of the Zebrafish Pallium. Frontiers in Cell and Developmental Biology. 10:1-11.

Ribeye-A

Ribeye-A

Ribeye-A

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Anti-Ribeye-A labels synapses

Ribeye-A protein is a scaffolding component in the ribbon where vesicles are tethered, its antibody has been found to label synapses (Shankhwar et al., 2022). Many spots were found across the outer plexiform layer, inner nuclear layer, inner plexiform layer and ganglion cell layer in the killifish.

Rabbit Polyclonal anti-Ribeye-A (Nicolson lab, Cat#Custom, dilution 1:100)


images

labels these retinal cell types

Synapses

key publications

Shankhwar S, Schwarz K, Katiyar R, Jung M, Maxeiner S, Südhof TC and Schmitz F. 2022. RIBEYE B-Domain Is Essential for RIBEYE A-Domain Stability and Assembly of Synaptic Ribbons. Frontiers in Molecular Neuroscience. 15:1-26.

Sex determining region Y-box (Sox2)

Sex determining region Y-box (Sox2)

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Anti-Sox2 labels progenitors

Sex determining region Y-box 2 (Sox2) antibody binds to the transcription factor Sox2 which forms OCT4 on DNA and has been found to label progenitors (Ahmed et al., 2015). In the killifish it labelled many cells in the inner nuclear and ganglion cell layer.

Rabbit polyclonal anti-Sox2 (Millipore, Cat#AB5603, dilution 1:200)

image

by Ryan MacDonald

labels these retinal cell types

Porgenitors

key publications

Ahmed TA, Ringuette R, Wallace VA and Griffith M. 2015. Autologous fibrin glue as an encapsulating scaffold for delivery of retinal progenitor cells. Frontiers in Bioengineering and Biotechnology. 2:1-11.

Synaptic vesicle Glycoprotein 2 (SV2)

Synaptic vesicle Glycoprotein 2 (SV2)

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Mouse monoclonal anti-SV2 (Hybridoma Bank, Cat#AB_2315387, dilution 1:100)

image

by Ryan MacDonald

labels these retinal cell types

Synapses

Tumor protein P53

Tumor protein P53

Tumor protein P53

ABOUT THIS ANTIBODY

Anti-P53 labels apoptosis

Tumor protein P53 is a sequence specific transcription factor which is being activated when cells are under stress. P53 antibody stains cell death in mice (Dong et al., 2019). In killifish it labelled one cell in the outer nuclear layer (white arrow).

Rabbit Polyclonal anti-P53 (Proteintech, Cat#21891-1-AP, dilution 1:100)

image

by Eva-Maria Breitenbach

Section of 4 mpf female killifish sodium citrate antigen retrieval anti-P53 (green) and DAPI (blue)

PS = photoreceptors, ONL = outer nuclear layer, OPL = outer plexiform layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer

labels these retinal cell types

Apoptosis

key publications

Dong S, Ji J, Hu L and Wang H. 2019. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sciences. 227:20-29.